| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92805-0.02ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28160 | |||
| FITC | NBP1-28159 | |||
| PE | NBP1-28161 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61697-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35293-1mg | |||
| Carrier-Free | NBP2-35293-500ug | |||
![Flow Cytometry: Integrin beta 2/CD18 Antibody (2E6) [NBP2-22433] - Analysis mouse splenocytes (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-2E6-Flow-Cytometry-NBP2-22433-img0001.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (2E6) [NBP2-22433] - Analysis mouse splenocytes (green) compared to an isotype control (blue).")
![Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-7I7B10-Western-Blot-NBP3-16669-img0002.jpg "Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-7I7B10-Western-Blot-NBP3-16669-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts from normal (control) and ITCH/AIP4 knockout (KO) 293T cells, using ITCH/AIP4 antibody (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/ITCH-AIP4-Antibody-7I7B10-Knockout-Validated-NBP3-16669-img0001.jpg)
![Western Blot: CHMP4B Antibody - BSA Free [NBP2-92805] - Western blot analysis of lysates from Mouse spleen using CHMP4B Rabbit pAb at 1:400 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit.Exposuretime:90s.](http://images.novusbio.com/fullsize/nbp2-92805_rabbit-polyclonal-chmp4b-antibody-42202514275243.jpg%3Ft=1701396936 "Western Blot: CHMP4B Antibody - BSA Free [NBP2-92805] - Western blot analysis of lysates from Mouse spleen using CHMP4B Rabbit pAb at 1:400 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit.Exposuretime:90s.")
![Immunocytochemistry/ Immunofluorescence: CHMP4B Antibody - BSA Free [NBP2-92805] - Immunofluorescence analysis of NIH/3T3 cells using CHMP4B Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92805_rabbit-polyclonal-chmp4b-antibody-4220251430471.jpg%3Ft=1701396936)
![Western Blot: ATPase Na+/K+ beta 3 Antibody (0B7M7) [NBP3-15829] - Analysis of extracts of various cell lines, using ATPase Na+/K+ beta 3 Rabbit mAb (NBP3-15829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-0B7M7-Western-Blot-NBP3-15829-img0002.jpg "Western Blot: ATPase Na+/K+ beta 3 Antibody (0B7M7) [NBP3-15829] - Analysis of extracts of various cell lines, using ATPase Na+/K+ beta 3 Rabbit mAb (NBP3-15829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: ATPase Na+/K+ beta 3 Antibody (0B7M7) [NBP3-15829] - Human liver cancer using ATPase Na+/K+ beta 3 Rabbit mAb (NBP3-15829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-0B7M7-Immunohistochemistry-Paraffin-NBP3-15829-img0001.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-UNLB followed by Mouse Anti-Rat IgG2b-AF488.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0009.jpg "Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-UNLB followed by Mouse Anti-Rat IgG2b-AF488.")
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the Biotin conjugate of NBP1-28158. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0006.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the FITC conjugate of NBP1-28158. Staining of less or equal to ug/10^6 BALB/c spleen cells with Rat Anti-Mouse MHC II-FITC, following which small lymphocytes were gated and analyzed on a flow cytometer.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0007.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the PE conjugate of NBP1-28158. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0008.jpg)
![Western Blot: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis using CD191 mAb against human CD191 (AA: extra mix) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-Western-Blot-NBP2-61697-img0003.jpg "Western Blot: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis using CD191 mAb against human CD191 (AA: extra mix) recombinant protein. (Expected MW is 37 kDa)")
![Flow Cytometry: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis of Ramos cells using CD191 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-Flow-Cytometry-NBP2-61697-img0002.jpg)
![Western Blot: CCR1 Antibody (8E10G6) [NBP2-61697] - Analysis using CD191 mAb against HEK293 (1) and CD191 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-Western-Blot-NBP2-61697-img0004.jpg)
![ELISA: CCR1 Antibody (8E10G6) [NBP2-61697] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CCR1-Antibody-8E10G6-ELISA-NBP2-61697-img0001.jpg)
![SDS-Page: Rat EGF Protein [NBP2-35293]](http://images.novusbio.com/fullsize/Rat-EGF-Protein-SDS-Page-NBP2-35293-img0001.jpg "SDS-Page: Rat EGF Protein [NBP2-35293]")
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (61D3) [NBP1-28436] - Hooded seal bronchoalveolar lavage cells were stained with Mouse Anti-Human CD14-UNLB followed by a secondary antibody and DRAQ5. Image from Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J. Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 2013;8(7):e70186.](http://images.novusbio.com/fullsize/CD14-Antibody-61D3-Immunocytochemistry-Immunofluorescence-NBP1-28436-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD14 Antibody (61D3) [NBP1-28436] - Hooded seal bronchoalveolar lavage cells were stained with Mouse Anti-Human CD14-UNLB followed by a secondary antibody and DRAQ5. Image from Larsen AK, Nymo IH, Boysen P, Tryland M, Godfroid J. Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro. PLoS One. 2013;8(7):e70186.")
![Flow Cytometry: CD14 Antibody (61D3) [NBP1-28436] - Analysis using the Allophycocyanin conjugate of NBP1-28436. Multiple staining of peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD14-Antibody-61D3-Flow-Cytometry-NBP1-28436-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR11 Antibody [NLS1442] - Anti-CCRL1 / CCR11 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 24-32 ug/ml.](http://images.novusbio.com/fullsize/CCR11-Antibody-Immunohistochemistry-Paraffin-NLS1442-img0003.jpg "Immunohistochemistry-Paraffin: CCR11 Antibody [NLS1442] - Anti-CCRL1 / CCR11 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 24-32 ug/ml.")
![Immunohistochemistry-Paraffin: CCR11 Antibody [NLS1442] - Analysis of anti-ACKR4 / CCRL1 / CCR11 antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/CCR11-Antibody-Immunohistochemistry-Paraffin-NLS1442-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR11 Antibody [NLS1442] - Anti-ACKR4 / CCRL1 / CCR11 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR11-Antibody-Immunohistochemistry-Paraffin-NLS1442-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR11 Antibody [NLS1442] - Anti-CCRL1 / CCR11 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 24-32 ug/ml.](http://images.novusbio.com/fullsize/CCR11-Antibody-Immunohistochemistry-Paraffin-NLS1442-img0004.jpg)
![Western Blot: SLCO1A2 Antibody [NBP2-85770] - WB Suggested Anti-SLCO1A2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysateSLCO1A2 is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells](http://images.novusbio.com/fullsize/SLCO1A2-Antibody-Western-Blot-NBP2-85770-img0002.jpg "Western Blot: SLCO1A2 Antibody [NBP2-85770] - WB Suggested Anti-SLCO1A2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysateSLCO1A2 is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells")
![Immunohistochemistry-Paraffin: SLCO1A2 Antibody [NBP2-85770] - Rabbit Anti-SLCO1A2 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver. Observed Staining: Membrane, Cytoplasm in hepatocytes, very strong, moderate tissue distribution. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 -2.0 sec.](http://images.novusbio.com/fullsize/SLCO1A2-Antibody-Immunohistochemistry-Paraffin-NBP2-85770-img0001.jpg)
![Western Blot: SLCO1A2 Antibody [NBP2-85770] - Host: Rabbit. Target Name: SLCO1A2. Sample Tissue: Human PANC1 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/SLCO1A2-Antibody-Western-Blot-NBP2-85770-img0003.jpg)
![Western Blot: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Western blot analysis of CD-M6PR on different lysates with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: PC-12 cell lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (40 ug/Lane) Lane 4: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 37/46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32555) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32555_rabbit-cd-m6pr-mab-psh01-06-278202416285299.jpg "Western Blot: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Western blot analysis of CD-M6PR on different lysates with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: PC-12 cell lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (40 ug/Lane) Lane 4: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 37/46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32555) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32555) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32555_rabbit-cd-m6pr-mab-psh01-06-298202414333687.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-278202415212472.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202410334035.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202416453237.jpg)