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![Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TNFSF13B-Antibody-5A8-Flow-Cytometry-NBP1-97639-img0001.jpg "Flow Cytometry: BAFF/BLyS/TNFSF13B Antibody (5A8) [NBP1-97639] - HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 10^5) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.")
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.")
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Separation of human CD22 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0002.jpg)
![Western Blot: Mouse Monoclonal Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - The reactivity of S-HCL-1 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody S-HCL-1 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).CD22 was detected at ~110-125 kDa in Daudi cell line.](http://images.novusbio.com/fullsize/antibody/nbp3-09167_mouse-monoclonal-siglec-2-cd22-antibody-s-hcl-1-western-blot-11122024122650..jpg)
![Flow Cytometry: NTB-A/SLAMF6/CD352 Antibody (hsF6.4.20) [NBP3-09135] - Separation of lymphocytes stained using anti-CD352 (hsF6.4.20) purified antibody (concentration in sample 0,56 ug/ml, GAM-APC, red-filled) from lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/NTB-A-SLAMF6-CD352-Antibody-hsF6-4-20-Flow-Cytometry-NBP3-09135-img0002.jpg "Flow Cytometry: NTB-A/SLAMF6/CD352 Antibody (hsF6.4.20) [NBP3-09135] - Separation of lymphocytes stained using anti-CD352 (hsF6.4.20) purified antibody (concentration in sample 0,56 ug/ml, GAM-APC, red-filled) from lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Flow Cytometry: NTB-A/SLAMF6/CD352 Antibody (hsF6.4.20) [NBP3-09135] - Surface staining pattern of human peripheral whole blood stained using anti-human CD352 (hsF6.4.20) Purified antibody (concentration in sample 0,56 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/NTB-A-SLAMF6-CD352-Antibody-hsF6-4-20-Flow-Cytometry-NBP3-09135-img0001.jpg)
![Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-278202415295589.jpg "Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD79A Antibody (PDM0-23) [NBP3-32168] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD79A antibody (NBP3-32168) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32168) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-29820241435290.jpg)
![Immunohistochemistry-Paraffin: NTB-A/SLAMF6/CD352 Antibody (005) [NBP2-90027] - Staining of human SLAMF6 in human appendix with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NTB-A-SLAMF6-CD352-Antibody-005-Immunohistochemistry-Paraffin-NBP2-90027-img0003.jpg "Immunohistochemistry-Paraffin: NTB-A/SLAMF6/CD352 Antibody (005) [NBP2-90027] - Staining of human SLAMF6 in human appendix with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: NTB-A/SLAMF6/CD352 Antibody (005) [NBP2-90027] - Staining of human SLAMF6 in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NTB-A-SLAMF6-CD352-Antibody-005-Immunohistochemistry-Paraffin-NBP2-90027-img0001.jpg)
![Immunohistochemistry-Paraffin: NTB-A/SLAMF6/CD352 Antibody (005) [NBP2-90027] - Staining of human SLAMF6 in human spleen with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/NTB-A-SLAMF6-CD352-Antibody-005-Immunohistochemistry-Paraffin-NBP2-90027-img0002.jpg)
![Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-278202416331267.jpg "Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD3 Antibody (PD01-43) [NBP3-32140] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-29820241492648.jpg)
![Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-278202416331259.jpg "Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32136) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-298202414115429.jpg)
![Western Blot: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Western blot analysis of Fc gamma RIIA/CD32a on different lysates with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Raji cell lysate Lane 3: HL-60 cell lysate Lane 4: Human lung tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 35 kDa Observed band size: 35-42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33141) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33141_rabbit-fc-gamma-riia-cd32a-mab-je64-17-28820241423311.jpg "Western Blot: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Western blot analysis of Fc gamma RIIA/CD32a on different lysates with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Raji cell lysate Lane 3: HL-60 cell lysate Lane 4: Human lung tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 35 kDa Observed band size: 35-42 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33141) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33141) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33141_rabbit-fc-gamma-riia-cd32a-mab-je64-17-29820241426266.jpg)
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0002.jpg "Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Separation of human CD268 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).")
![Flow Cytometry: BAFFR/TNFRSF13C Antibody (11C1) [NBP3-09142] - Surface staining pattern of human peripheral whole blood stained using anti-human CD268 (11C1) purified antibody (concentration in sample 0,6 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/BAFFR-TNFRSF13C-Antibody-11C1-Flow-Cytometry-NBP3-09142-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CRACC/SLAMF7 Antibody (1B9) [H00057823-M01] - Analysis of monoclonal antibody to SLAMF7 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-1B9-Immunocytochemistry-Immunofluorescence-H00057823-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: CRACC/SLAMF7 Antibody (1B9) [H00057823-M01] - Analysis of monoclonal antibody to SLAMF7 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: CRACC/SLAMF7 Antibody (1B9) [H00057823-M01] - Detection limit for recombinant GST tagged SLAMF7 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CRACC-SLAMF7-Antibody-1B9-Sandwich-ELISA-H00057823-M01-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with NBP3-12048 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12048_rabbit-monoclonal-marco-antibody-plk1-chimeric-74202522113028.png "Immunocytochemistry /Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with NBP3-12048 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")