| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92949-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35293-1mg | |||
| Carrier-Free | NBP2-35293-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-85473-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12068-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81085-0.025mg | |||
![Flow Cytometry: Integrin beta 2/CD18 Antibody (2E6) [NBP2-22433] - Analysis mouse splenocytes (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-2E6-Flow-Cytometry-NBP2-22433-img0001.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (2E6) [NBP2-22433] - Analysis mouse splenocytes (green) compared to an isotype control (blue).")
![Western Blot: Calpain 2 Antibody [NBP2-92949] - Analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using Calpain 2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Knockout-Validated-NBP2-92949-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Calpain 2 Antibody [NBP2-92949] - Analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using Calpain 2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in")
![SDS-Page: Rat EGF Protein [NBP2-35293]](http://images.novusbio.com/fullsize/Rat-EGF-Protein-SDS-Page-NBP2-35293-img0001.jpg "SDS-Page: Rat EGF Protein [NBP2-35293]")
![Western Blot: TrkA Antibody (OTI5B6) - Azide and BSA Free [NBP2-74623] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TrkA.](http://images.novusbio.com/fullsize/TrkA-Antibody-OTI5B6-Azide-and-BSA-Free-Western-Blot-NBP2-74623-img0001.jpg "Western Blot: TrkA Antibody (OTI5B6) - Azide and BSA Free [NBP2-74623] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TrkA.")
![Immunohistochemistry: TrkA Antibody (OTI5B6) - Azide and BSA Free [NBP2-74623] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TrkA-Antibody-OTI5B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74623-img0002.jpg)
![Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.](http://images.novusbio.com/fullsize/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0006.jpg "Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.")
![Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - The Bouins solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to IHC with anti mouse E-Selectin using NB110-85473.](http://images.novusbio.com/fullsize/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0005.jpg)
![Western Blot: Leukotriene B4 R1 Antibody (JE58-77) [NBP3-32529] - Western blot analysis of Leukotriene B4 R1 on different lysates with Rabbit anti-Leukotriene B4 R1 antibody (NBP3-32529) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (no heat) Lysates/proteins at 20 ug/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32529) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32529_rabbit-leukotriene-b4-r1-mab-je58-77-278202415295592.jpg "Western Blot: Leukotriene B4 R1 Antibody (JE58-77) [NBP3-32529] - Western blot analysis of Leukotriene B4 R1 on different lysates with Rabbit anti-Leukotriene B4 R1 antibody (NBP3-32529) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (no heat) Lysates/proteins at 20 ug/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32529) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Leukotriene B4 R1 Antibody (JE58-77) [NBP3-32529] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Leukotriene B4 R1 antibody (NBP3-32529) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32529) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32529_rabbit-leukotriene-b4-r1-mab-je58-77-29820241433366.jpg)
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-1572024145956..jpg "Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE1 antibody at 1/500 dilution.Lane 1: MCF-7 cell lysateLane 2: SW480 cell lysateLane 3: A431 cell lysateLane 4: HUVEC cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot: LYVE-1 Antibody (JF0979) [NBP2-66940] - Western blot analysis of LYVE-1 on different lysates with Rabbit anti-LYVE-1 antibody at 1/1,000 dilution.Lane 1: A549-si NT cell lysateLane 2: A549-si LYVE-1 cell lysateLysates/proteins at 10 µg/Lane.Predicted band size: 35 kDaObserved band size: 35 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-66940 was shown to specifically react with LYVE-1 in A549-si NT cells. Weakened band was observed when A549-si LYVE-1 sample was tested. A549-si NT and Hela-si LYVE-1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-29, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66940_rabbit-monoclonal-lyve-1-antibody-jf0979-western-blot-15720241537..jpg)
![Flow Cytometry: LYVE-1 Antibody (JF0979) [NBP2-66940] - Analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/LYVE-1-Antibody-JF0979-Flow-Cytometry-NBP2-66940-img0001.jpg)
![Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) [NBP3-09130] - Separation of murine bone marrow cells stained using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 ug/ml, DAR APC) from murine bone marrow cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/antibody/nbp3-09130_rat-monoclonal-integrin-alpha-5-cd49e-antibody-5h10-27-mfr5-flow-cytometry-12122024143343..jpg "Flow Cytometry: Rat Monoclonal Integrin alpha 5/CD49e Antibody (5H10-27 (MFR5)) [NBP3-09130] - Separation of murine bone marrow cells stained using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 ug/ml, DAR APC) from murine bone marrow cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).")
![Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-288202412493428.jpg "Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-29820241420920.jpg)
![Western Blot: DDR2 Antibody (JE31-45) [NBP3-32260] - Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (NBP3-32260) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: SH-SY5Y cell lysate (40 ug/Lane) Lane 3: L929 cell lysate (40 ug/Lane) Lane 4: NIH/3T3 cell lysate (40 ug/Lane) Lane 5: U-2 OS cell lysate (15 ug/Lane) Lane 6: A375 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (10 ug/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32260) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32260_rabbit-ddr2-mab-je31-45-27820241631680.jpg "Western Blot: DDR2 Antibody (JE31-45) [NBP3-32260] - Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (NBP3-32260) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: SH-SY5Y cell lysate (40 ug/Lane) Lane 3: L929 cell lysate (40 ug/Lane) Lane 4: NIH/3T3 cell lysate (40 ug/Lane) Lane 5: U-2 OS cell lysate (15 ug/Lane) Lane 6: A375 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (10 ug/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32260) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DDR2 Antibody (JE31-45) [NBP3-32260] - Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-DDR2 antibody (NBP3-32260) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32260) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32260_rabbit-ddr2-mab-je31-45-298202414161855.jpg)
![Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-FR4D11-Chimeric-Western-Blot-NBP3-12068-img0001.jpg "Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) - Chimeric - Azide and BSA Free [NBP3-12068] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12068 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12068, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12068_rabbit-monoclonal-neprilysin-cd10-antibody-fr4d11-chimeric-74202522141215.png)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81085-img0001.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")