| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-225SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81113-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35895-100ug | |||
| Carrier-Free | NBP2-35895-500ug | |||
| Carrier-Free | NBP2-35895-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB38331-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48393 | |||
| Azide and BSA Free | NBP3-11562 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11606 | |||
![Western Blot: CARD12 Antibody [NB500-225] - Immunoblot analysis of cN-II and Ipaf (CARD12) protein expression in different human cell lines. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121525) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CARD12-Antibody-Western-Blot-NB500-225-img0002.jpg "Western Blot: CARD12 Antibody [NB500-225] - Immunoblot analysis of cN-II and Ipaf (CARD12) protein expression in different human cell lines. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121525) licensed under a CC-BY license.")
![Western Blot: CARD12 Antibody [NB500-225] - Western Blot analysis of CLAN in lung lysate with anti-CLAN at a 1:500 dilution. Detects CLAN-A and CLAN-B isoforms.](http://images.novusbio.com/fullsize/CARD12-Antibody-Western-Blot-NB500-225-img0001.jpg)
![Western Blot: CARD12 Antibody - BSA Free [NB500-225] - In vitro interaction between cN-II & Ipaf B.After loading Ni-talon beads with E. coli “BL21(DE3)” crude extract without (■) or with cN-II (●), beads were washed & E. coli “BL21 Rosetta(DE3)” extracts containing Ipaf B were loaded. Elution buffer containing 250 mM imidazole was used from fraction 21 to detach His-tagged cN-II & elute proteins. A) proteins elution profile; B) immunoblotting with CARD12 & cN-II antibodies on elution fractions 3, 22, 24, 25 & 26 from chromatography. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25811392), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-225_rabbit-polyclonal-card12-antibody-31020241539456.jpg)
![Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-278202415212446.jpg "Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32154) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-298202414161888.jpg)
![Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunocytochemistry analysis of U-937 (positive) and HT-29 (negative) labeling CD43/Sialophorin with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-2920241114826.jpg)
![Western Blot: RHD Antibody [NBP2-84247] - Host: Rabbit. Target Name: RHD. Sample Type: Human Fetal Liver. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/RHD-Antibody-Western-Blot-NBP2-84247-img0004.jpg "Western Blot: RHD Antibody [NBP2-84247] - Host: Rabbit. Target Name: RHD. Sample Type: Human Fetal Liver. Antibody Dilution: 1.0ug/ml")
![Western Blot: RHD Antibody [NBP2-84247] - WB Suggested Anti-RHD Antibody. Titration: 1.0 ug/ml. Positive Control: RPMI-8226 Whole Cell.RHD is strongly supported by BioGPS gene expression data to be expressed in RPMI 8226](http://images.novusbio.com/fullsize/RHD-Antibody-Western-Blot-NBP2-84247-img0001.jpg)
![Western Blot: RHD Antibody [NBP2-84247] - Host: Rabbit. Target Name: RHD. Sample Type: 293T. Antibody Dilution: 1.0ug/mlRHD is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells](http://images.novusbio.com/fullsize/RHD-Antibody-Western-Blot-NBP2-84247-img0002.jpg)
![Western Blot: RHD Antibody [NBP2-84247] - Host: Rabbit. Target Name: RHD. Sample Type: Human Fetal Brain. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/RHD-Antibody-Western-Blot-NBP2-84247-img0003.jpg)
![Western Blot: RHD Antibody [NBP2-84247] - Host: Rabbit. Target Name: RHD. Sample Type: Human Adult Placenta. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/RHD-Antibody-Western-Blot-NBP2-84247-img0005.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202492029.jpg%3Fv=1666701381 "Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).")
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of HepG2 cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-10420249141939.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202491625.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024920223.jpg%3Fv=1666701381)
![ELISA: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024916215.jpg%3Fv=1666701381)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Western-Blot-NBP2-81113-img0002.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Flow-cytometry using the Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (NBP2-81113, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Flow-Cytometry-NBP2-81113-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric - Azide and BSA Free [NBP2-81113] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with NBP2-81113 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81113, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81113_rabbit-monoclonal-fas-tnfrsf6-cd95-antibody-r-125224-chimeric-74202522141233.png)
![Functional: Human IL1 beta Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL1-beta-Protein-Functional-NBP2-35895-img0001.jpg "Functional: Human IL1 beta Protein [NBP2-35895]")
![SDS-Page: Human IL-1 beta/IL-1F2 Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35895-img0002.jpg)
![Western Blot: NALP3 Peptide [NBP1-77080PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.](http://images.novusbio.com/fullsize/NALP3-Peptide-Western-Blot-NBP1-77080PEP-img0001.jpg "Western Blot: NALP3 Peptide [NBP1-77080PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.")
![Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (Bra7G) [NBP2-44650] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (Bra7G) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot .](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Bra7G-Immunocytochemistry-Immunofluorescence-NBP2-44650-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (Bra7G) [NBP2-44650] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (Bra7G) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot .")
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (Bra7G) [NBP2-44650] - Human Spleen stained with CD43 Monoclonal Antibody (Bra7G).](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Bra7G-Immunohistochemistry-Paraffin-NBP2-44650-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (SPM490) [NBP2-45305] - ICC/IF staining of U937 cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-SPM490-Immunofluorescence-NBP2-45305-img0002.jpg "Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (SPM490) [NBP2-45305] - ICC/IF staining of U937 cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Paraffin: SSEA-1 Antibody (SPM490) [NBP2-45305] - Human Hodgkin's Lymphoma stained with CD15 Monoclonal Antibody (SPM490)](http://images.novusbio.com/fullsize/SSEA-1-Antibody-SPM490-Immunohistochemistry-Paraffin-NBP2-45305-img0001.jpg)
![Western Blot: Complement Component C5a Antibody (D4) [NBP3-41581] - Sample: Rat Serum Primary Ab: 3ug/ml NBP3-41581; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41581_mouse-complement-component-c5a-mab-d4-61220242124321.jpg "Western Blot: Complement Component C5a Antibody (D4) [NBP3-41581] - Sample: Rat Serum Primary Ab: 3ug/ml NBP3-41581; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Complement Component C5a Antibody (D4) [NBP3-41581] - Sample: Lane1: Human Serum; Lane2: Human Plasma Primary Ab: 5ug/ml NBP3-41581; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41581_mouse-complement-component-c5a-mab-d4-612202421373023.jpg)
![Immunohistochemistry-Paraffin: Complement Component C5a Antibody (D4) [NBP3-41581] - DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 30ug/ml NBP3-41581; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41581_mouse-complement-component-c5a-mab-d4-61220242058817.jpg)