| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09027-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81099-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12062-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81105-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50177-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81040-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52641-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08990-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75195-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81083-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81055-0.025mg | |||
![Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/IL-16-Antibody-14-1-Chimeric-Flow-Cytometry-NBP3-09027-img0001.jpg "Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric [NBP3-12062] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of TL2.1 (NBP3-12062) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12062, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12062-img0001.jpg "Immunocytochemistry/Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric [NBP3-12062] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of TL2.1 (NBP3-12062) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12062, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Western Blot using Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 [NBP2-81105] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated. NBP2-81105 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.](http://images.novusbio.com/fullsize/Lymphotoxin-alpha-TNF-beta-Antibody-3F12-2D3-Chimeric-Western-Blot-NBP2-81105-img0002.jpg "Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Western Blot using Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 [NBP2-81105] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated. NBP2-81105 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.")
![Flow Cytometry: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Flow-cytometry using the Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. U937 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 3F12.2D3 (NBP2-81105, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Lymphotoxin-alpha-TNF-beta-Antibody-3F12-2D3-Chimeric-Flow-Cytometry-NBP2-81105-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LAG-3-Antibody-C9B7W-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09044-img0002.jpg "Immunocytochemistry/Immunofluorescence: LAG-3 Antibody (C9B7W) - Chimeric [NBP3-09044] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of C9B7W (NBP3-09044) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09044, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![ELISA: LAG-3 Antibody (C9B7W) - Chimeric - Azide and BSA Free [NBP3-09044] - ELISA Plate coated with recombinant mouse LAG-3 Fc-Fusion Protein at a concentration of 2 ug/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using NBP3-09044. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/nbp3-09044_rabbit-monoclonal-lag-3-antibody-c9b7w-chimeric-74202522113011.png)
![Western Blot: IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040] - Western Blot using IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040]. HeLa cell lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H [NBP2-81040] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. NBP2-81040 successfully detected IL18 in HeLa cell lysate samples.](http://images.novusbio.com/fullsize/IL-18-IL-1F4-Antibody-125-2H-Western-Blot-NBP2-81040-img0001.jpg "Western Blot: IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040] - Western Blot using IL-18/IL-1F4 Antibody (125-2H) [NBP2-81040]. HeLa cell lysate samples (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H [NBP2-81040] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. NBP2-81040 successfully detected IL18 in HeLa cell lysate samples.")
![Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-3D6-112-recombinant-version-Flow-Cytometry-NBP3-08990-img0001.jpg "Flow Cytometry: Siglec-1/CD169 Antibody (3D6.112 (recombinant version)) [NBP3-08990] - Murine bone marrow-derived macrophages (BMDMs) were stained with anti-Siglec-1/CD169 antibody NBP3-08990 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a Siglec-1/CD169-positive population is apparent.")
![Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-Flow-Cytometry-NBP2-75195-img0002.jpg "Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody")
![ELISA: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Binding curve of anti-B7-H3 antibody MJ18 (NBP2-75195) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-75195. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-ELISA-NBP2-75195-img0001.jpg)
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-MEL-14-Flow-Cytometry-NBP2-81083-img0001.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083] - Flow-cytometry using the L-Selectin/CD62L Antibody (MEL-14) [NBP2-81083]. Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of MEL-14 (NBP2-81083, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Flow Cytometry: L-Selectin/CD62L Antibody (MEL-14) - Chimeric - Azide and BSA Free [NBP2-81083] - Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or NBP2-81083 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp2-81083_rabbit-monoclonal-l-selectin-cd62l-antibody-mel-14-74202522141216.png)
![Immunocytochemistry/Immunofluorescence: CD38 Antibody (AT13/5) [NBP2-81055] - Immunofluorescence staining of fixed Daudi cells with anti-CD38 antibody AT13/5 [NBP2-81055]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of AT13/5 [NBP2-81055] at 10 ug/mL for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81055, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD38-Antibody-AT13-5-Immunocytochemistry-Immunofluorescence-NBP2-81055-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD38 Antibody (AT13/5) [NBP2-81055] - Immunofluorescence staining of fixed Daudi cells with anti-CD38 antibody AT13/5 [NBP2-81055]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of AT13/5 [NBP2-81055] at 10 ug/mL for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81055, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CD38 Antibody (AT13/5) [NBP2-81055] - Western Blot using anti-CD38 antibody (AT13/5) [NBP2-81055]. Human spleen lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of AT13/5 [NBP2-81055] at 1 ug/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD38 is 34.3 kDa, but this protein is glycosylated at several residues. NBP2-81055 successfully detected CD38 in human spleen lysate.](http://images.novusbio.com/fullsize/CD38-Antibody-AT13-5-Western-Blot-NBP2-81055-img0001.jpg)