| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37912-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88965-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26700SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88023-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83094-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-88949SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89034-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76702-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61806-0.025ml | |||
![Immunohistochemistry-Paraffin: WASF2 Antibody [NBP2-37912] - Analysis in human esophagus and skeletal muscle tissues. Corresponding WASF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WASF2-Antibody-Immunohistochemistry-Paraffin-NBP2-37912-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: WASF2 Antibody [NBP2-37912] - Analysis in human esophagus and skeletal muscle tissues. Corresponding WASF2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: WASF2 Antibody [NBP2-37912] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antiibody staining is shown in green.](http://images.novusbio.com/fullsize/WASF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37912-img0004.jpg)
![Immunohistochemistry-Paraffin: WASF2 Antibody [NBP2-37912] - Staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/WASF2-Antibody-Immunohistochemistry-Paraffin-NBP2-37912-img0011.jpg)
![Immunohistochemistry-Paraffin: WASF2 Antibody [NBP2-37912] - Staining of human placenta shows strong cytoplasmic positivity in endothelial cells.](http://images.novusbio.com/fullsize/WASF2-Antibody-Immunohistochemistry-Paraffin-NBP2-37912-img0006.jpg)
![Immunohistochemistry-Paraffin: WASF2 Antibody [NBP2-37912] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/WASF2-Antibody-Immunohistochemistry-Paraffin-NBP2-37912-img0007.jpg)
![Immunohistochemistry-Paraffin: WASF2 Antibody [NBP2-37912] - Staining of human Fallopian tube shows moderate cytoplasmic positivity in glandular cells](http://images.novusbio.com/fullsize/WASF2-Antibody-Immunohistochemistry-Paraffin-NBP2-37912-img0008.jpg)
![Immunohistochemistry-Paraffin: WASF2 Antibody [NBP2-37912] - Staining of human skeletal muscle shows low positivity in myocytes as expected.](http://images.novusbio.com/fullsize/WASF2-Antibody-Immunohistochemistry-Paraffin-NBP2-37912-img0009.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0009.jpg "Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human testis.")
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon, kidney, testis and urinary bladder using Anti-MKKS antibody NBP1-92122 (A) shows similar protein distribution across tissues to independent antibody NBP1-92123 (B).](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0006.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human colon.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0007.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0008.jpg)
![Immunohistochemistry-Paraffin: MKKS Antibody [NBP1-92122] - Staining of human kidney.](http://images.novusbio.com/fullsize/MKKS-Antibody-Immunohistochemistry-Paraffin-NBP1-92122-img0010.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-92122_rabbit-polyclonal-mkks-antibody-29520251625315.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88965-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining in human prostate and pancreas tissues using anti-CD276 antibody. Corresponding CD276 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0013.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88965-img0015.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88965-img0005.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP1-88023] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88023-img0009.jpg "Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP1-88023] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.")
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0013.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0010.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0011.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in lymphoid cells and in glandular cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HIV-1 Rev binding protein Antibody [NBP1-91991] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.](http://images.novusbio.com/fullsize/HIV-1-Rev-binding-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91991-img0009.jpg "Immunocytochemistry/Immunofluorescence: HIV-1 Rev binding protein Antibody [NBP1-91991] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.")
![Immunohistochemistry-Paraffin: HIV-1 Rev binding protein Antibody [NBP1-91991] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/HIV-1 Rev binding protein Antibody-Immunohistochemistry-Paraffin-NBP1-91991-img0012.jpg)
![Western Blot: HIV-1 Rev binding protein Antibody [NBP1-91991] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/HIV-1-Rev-binding-protein-Antibody-Western-Blot-NBP1-91991-img0010.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-91991_rabbit-polyclonal-hiv-1-rev-binding-protein-antibody-295202516111819.jpg)
![Western Blot: IRAK3 Antibody [NBP1-83094] - Analysis in control (vector only transfected HEK293T lysate) and IRAK3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/IRAK3-Antibody-Western-Blot-NBP1-83094-img0005.jpg "Western Blot: IRAK3 Antibody [NBP1-83094] - Analysis in control (vector only transfected HEK293T lysate) and IRAK3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: IRAK3 Antibody [NBP1-83094] - Immunofluorescent staining of human cell line SiHa shows localization to vesicles.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83094-img0003.jpg)
![Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-83094] - Staining of human skeletal muscle shows low positivity in myocytes as expected.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunohistochemistry-Paraffin-NBP1-83094-img0008.jpg)
![Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-83094] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunohistochemistry-Paraffin-NBP1-83094-img0004.jpg)
![Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-83094] - Staining of human gastrointestinal shows strong cytoplasmic positivity in peripheral leukocytes.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunohistochemistry-Paraffin-NBP1-83094-img0006.jpg)
![Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-83094] - Staining of human tonsil shows strong cytoplasmic positivity in germinal and non germinal center cells.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunohistochemistry-Paraffin-NBP1-83094-img0007.jpg)
![Western Blot: Dicer Antibody [NBP2-88949] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Dicer in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP2-88949-img0002.jpg "Western Blot: Dicer Antibody [NBP2-88949] - Total protein from human HeLa and A431 cells, mouse 3T3 cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Dicer in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-88949] - Analysis of a FFPE tissue section of the mouse prostate using 1:200 dilution of Dicer antibody (NBP2-88949). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated moderate staining of cell cytoplasm.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunohistochemistry-Paraffin-NBP2-88949-img0001.jpg)
![Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NBP1-89034] - Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Western-Blot-NBP1-89034-img0009.jpg "Western Blot: Fas Receptor/TNFRSF6/CD95 Antibody [NBP1-89034] - Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Analysis in human cell line HDLM-2.")
![Immunocytochemistry/Immunofluorescence: Fas Receptor/TNFRSF6/CD95 Antibody [NBP1-89034] - Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol. Antibody staining is show in green.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89034-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Staining in human rectum and skeletal muscle tissues using anti-Fas/TNFRSF6/CD95 antibody. Corresponding Fas/TNFRSF6/CD95 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NBP1-89034-img0013.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NBP1-89034] - Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Staining of human rectum shows high expression.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NBP1-89034-img0011.jpg)
![Immunohistochemistry-Paraffin: Fas Receptor/TNFRSF6/CD95 Antibody [NBP1-89034] - Fas/TNFRSF6/CD95 Antibody [NBP1-89034] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-Immunohistochemistry-Paraffin-NBP1-89034-img0012.jpg)
![Western Blot: TIM-4 Antibody [NBP1-76702] - Analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/TIM-4-Antibody-Western-Blot-NBP1-76702-img0008.jpg "Western Blot: TIM-4 Antibody [NBP1-76702] - Analysis of TIM-4 in RAW264.7 cell lysate with TIM-4 antibody at 2 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunofluorescence of TIM-4 in Jurkat cells with TIM-4 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76702_rabbit-polyclonal-tim-4-antibody-271120242074681.jpg)
![Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunocytochemistry of TIM-4 in Jurkat cells with TIM-4 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76702_rabbit-polyclonal-tim-4-antibody-2711202420392754.jpg)
![Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunofluorescence of TIM-4 in human spleen tissue with TIM-4 antibody at 20 ug/ml.Green: TIM-4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76702_rabbit-polyclonal-tim-4-antibody-271120241928879.gif)
![Immunohistochemistry: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunohistochemistry of TIM-4 in human spleen tissue with TIM-4 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76702_rabbit-polyclonal-tim-4-antibody-271120241926183.gif)
![Immunohistochemistry: TIM-4 Antibody - BSA Free [NBP1-76702] - Immunohistochemistry of TIM-4 in mouse liver tissue with TIM-4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76702_rabbit-polyclonal-tim-4-antibody-2711202419531672.gif)
![Western Blot: ACTR3 Antibody (3G2C9) [NBP2-61806] - Analysis using ACTR3 mAb against HEK293 (1) and ACTR3 (AA: 287-418)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ACTR3-Antibody-3G2C9-Western-Blot-NBP2-61806-img0005.jpg "Western Blot: ACTR3 Antibody (3G2C9) [NBP2-61806] - Analysis using ACTR3 mAb against HEK293 (1) and ACTR3 (AA: 287-418)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: ACTR3 Antibody (3G2C9) [NBP2-61806] - Analysis of cervical cancer tissues using ACTR3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ACTR3-Antibody-3G2C9-Immunohistochemistry-NBP2-61806-img0003.jpg)
![Western Blot: ACTR3 Antibody (3G2C9) [NBP2-61806] - Analysis using ACTR3 mAb against human ACTR3 (AA: 287-418) recombinant protein. (Expected MW is 41.1 kDa)](http://images.novusbio.com/fullsize/ACTR3-Antibody-3G2C9-Western-Blot-NBP2-61806-img0004.jpg)
![Western Blot: ACTR3 Antibody (3G2C9) [NBP2-61806] - Analysis using ACTR3 mouse mAb against NIH/3T3 (1) and A549 (2) cell lysate.](http://images.novusbio.com/fullsize/ACTR3-Antibody-3G2C9-Western-Blot-NBP2-61806-img0006.jpg)
![ELISA: ACTR3 Antibody (3G2C9) [NBP2-61806] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ACTR3-Antibody-3G2C9-ELISA-NBP2-61806-img0001.jpg)
![ELISA: ACTR3 Antibody (3G2C9) [NBP2-61806] - Analysis of Hela cells using ACTR3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ACTR3-Antibody-3G2C9-ELISA-NBP2-61806-img0002.jpg)
![Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-278202415212453.jpg "Western Blot: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Western blot analysis of RelA/NFkB p65 on different lysates with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/500 dilution. Lane 1: Hela cell lysate (control) Lane 2: Hela-RELA-KO (1) Lane 3: Hela-RELA-KO (2) Lysates/proteins at 35 ug/Lane. Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32648) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29820241435276.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-alpha for 20 minutes labeling RelA/NFkB p65 with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RelA/NFkB p65 antibody (NBP3-32648) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-29202410484811.jpg)
![Flow Cytometry: RelA/NFkB p65 Antibody (PSH0-27) [NBP3-32648] - Flow cytometric analysis of NIH/3T3 cells labeling RelA/NFkB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32648, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32648_rabbit-rela-nfkb-p65-mab-psh0-27-2920241637214.jpg)