| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-14758SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08924 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76843-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71855 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-43546-0.5mg | |||
| Biotin | NBP1-43546-0.1mg | |||
| PE/Cy5 | NBP1-43068-0.1mg | |||
| PE/Cy7 | NBP1-42940-0.1mg | |||
| PE/Cy7 | NBP1-42940-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43303-0.1mg | |||
| BSA Free | NBP1-43303-0.025mg | |||
![Flow (Cell Surface): CD5 Antibody (7.8) [NBP3-14758] - A surface stain was performed on Jurkat (panel A) and Raw264.7 (panel B) cells with human specific CD5 Antibody (7.8) NBP3-14758 (blue) and a matched isotype control MAB003 (orange). Cells were incubated in an antibody dilution of 1.0 ug/mL for 20 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/CD5-Antibody-7-8-Flow-Cell-Surface-NBP3-14758-img0001.jpg "Flow (Cell Surface): CD5 Antibody (7.8) [NBP3-14758] - A surface stain was performed on Jurkat (panel A) and Raw264.7 (panel B) cells with human specific CD5 Antibody (7.8) NBP3-14758 (blue) and a matched isotype control MAB003 (orange). Cells were incubated in an antibody dilution of 1.0 ug/mL for 20 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).")
![Western Blot: c-Maf Antibody [NBP3-14672] - Antibody: Rabbit anti-c-Maf recombinantmonoclonal antibody used at1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Myc Tagrecombinant monoclonal antibody.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP3-14672-img0002.jpg "Western Blot: c-Maf Antibody [NBP3-14672] - Antibody: Rabbit anti-c-Maf recombinantmonoclonal antibody used at1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Myc Tagrecombinant monoclonal antibody.")
![Immunohistochemistry: c-Maf Antibody [NBP3-14672] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-c-MAF recombinant monoclonalantibody used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-NBP3-14672-img0004.jpg)
![Western Blot: c-Maf Antibody [NBP3-14672] - Whole cell lysate (50 ug) from RPMI-8226, Hep-G2, 786-O, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-c-Maf recombinant monoclonal antibody (NBP3-14672lot 3) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP3-14672-img0001.jpg)
![Immunohistochemistry: c-Maf Antibody [NBP3-14672] - Sample: FFPE section of human tonsil. Antibody: Rabbitanti-c-MAF recombinant monoclonal antibodyused at 1:125. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-NBP3-14672-img0003.jpg)
![ICC/IF-c-Maf Antibody [NBP3-14672] - Section of RPMI-8226 cells. Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody [BLR045F] used at 1:125. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14672_rabbit-c-maf-mab-immunocytochemistry-immunofluorescence-2032024103528..jpg)
![Western Blot: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Western Blot Analysis of human spleen cell lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-2877R-Western-Blot-NBP3-07180-img0005.jpg "Western Blot: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Western Blot Analysis of human spleen cell lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).")
![Immunocytochemistry/Immunofluorescence: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Paraformaldehyde-fixed Raji cells stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R)followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-2877R-Immunocytochemistry-Immunofluorescence-NBP3-07180-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-2877R-Immunohistochemistry-Paraffin-NBP3-07180-img0002.jpg)
![Flow Cytometry: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R) followed by Goat anti-rabbitIgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-2877R-Flow-Cytometry-NBP3-07180-img0001.jpg)
![Western Blot: CD45RB Antibody (PTPRC/2877R) [NBP3-07180] - Western Blot Analysis of human Raji cell lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).](http://images.novusbio.com/fullsize/CD45RB-Antibody-PTPRC-2877R-Western-Blot-NBP3-07180-img0004.jpg)
![Western Blot: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Rabbit anti-CD3E recombinant monoclonal antibody [BLR174J] used at 1:1000.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BLR174J-Western-Blot-NBP3-14747-img0003.jpg "Western Blot: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Rabbit anti-CD3E recombinant monoclonal antibody [BLR174J] used at 1:1000.")
![Immunohistochemistry: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Rabbit anti-CD3E recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BLR174J-Immunohistochemistry-NBP3-14747-img0002.jpg)
![Flow Cytometry: CD3 epsilon Antibody (BLR174J) [NBP3-14747] - Rabbit anti-CD3E recombinant monoclonal antibody or isotype control (unshaded).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BLR174J-Flow-Cytometry-NBP3-14747-img0001.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0005.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (Lane 1: 0.25 ug/mL; Lane 2: 0.5 ug/mL and Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kDa.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: BAFF/BLyS/TNFSF13B Antibody [NBP1-76843] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BAFF/BLyS/TNFSF13B NBP1-76843 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BAFF-BLyS-TNFSF13B-Antibody-Western-Blot-NBP1-76843-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-BAFF/BLyS/TNFSF13B antibody at 1 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-271120242010580.jpg)
![Western Blot: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M). Loading: 15 ug of lysates per lane.Antibodies: BAFF/BLyS/TNFSF13B (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285892.jpg)
![Immunocytochemistry/ Immunofluorescence: BAFF/BLyS/TNFSF13B Antibody - BSA Free [NBP1-76843] - Validation of BAFF/BLyS/TNFSF13B in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF/BLyS/TNFSF13B with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76843_rabbit-polyclonal-baff-blys-tnfsf13b-antibody-2711202420285848.gif)
![Western Blot: BLNK Antibody (4C9) [NBP2-46577] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BLNK.](http://images.novusbio.com/fullsize/BLNK-Antibody-4C9-Western-Blot-NBP2-46577-img0007.jpg "Western Blot: BLNK Antibody (4C9) [NBP2-46577] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BLNK.")
![Immunohistochemistry: BLNK Antibody (4C9) [NBP2-46577] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-4C9-Immunohistochemistry-NBP2-46577-img0006.jpg)
![Immunohistochemistry: BLNK Antibody (4C9) [NBP2-46577] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-4C9-Immunohistochemistry-NBP2-46577-img0001.jpg)
![Immunohistochemistry: BLNK Antibody (4C9) [NBP2-46577] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-4C9-Immunohistochemistry-NBP2-46577-img0002.jpg)
![Immunohistochemistry: BLNK Antibody (4C9) [NBP2-46577] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-4C9-Immunohistochemistry-NBP2-46577-img0003.jpg)
![Immunohistochemistry: BLNK Antibody (4C9) [NBP2-46577] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-4C9-Immunohistochemistry-NBP2-46577-img0004.jpg)
![Immunohistochemistry: BLNK Antibody (4C9) [NBP2-46577] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-4C9-Immunohistochemistry-NBP2-46577-img0005.jpg)
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE/Cy7 conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 micrograms of Rat IgG2a ? Isotype Control PE-Cy7 (left) or 0.06 micrograms of Anti-Mouse IgM PE-Cy7 (right). Cells in the ly](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0008.jpg "Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE/Cy7 conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 micrograms of Rat IgG2a ? Isotype Control PE-Cy7 (left) or 0.06 micrograms of Anti-Mouse IgM PE-Cy7 (right). Cells in the ly")
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Staining of C57BL/6 splenocytes with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse IgM Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0001.jpg)
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Allophycocyanin conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.125 ug of Rat IgG2a K Isotype Control APC (left) or 0.125 ug of Anti-Mouse IgM APC (right). Cells in the small scatter](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0002.jpg)
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Allophycocyanin conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.125 ug of Rat IgG2a K Isotype Control APC (left) or 0.125 ug of Anti-Mouse IgM APC (right). Cells in the small scatter](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0003.jpg)
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Biotin conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgG2a kappa Isotype Control Biotin (left) or 0.06 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE .](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0004.jpg)
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of FITC conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.125 ug of Rat IgG2a K Isotype Control FITC (left) or 0.125 ug of Anti-Mouse IgM FITC (right). Total viable cells were used for ana](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0005.jpg)
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE conjugate of NBP1-43303. The II/41 antibody has been tested by flow cytometric mouse spleen cell suspension. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0006.jpg)
![Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE/Cy5 conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 ug of Rat IgG2a Isotype Control PE-Cy5 (left) or 0.06 ug of Anti-Mouse IgM PE-Cy5 (right). Total viable cells were used for](http://images.novusbio.com/fullsize/Mouse-IgM-Antibody-II-41-Flow-Cytometry-NBP1-43303-img0007.jpg)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-1063-img0007.jpg "Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1063-img0011.jpg)
![Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Antibody (2 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunohistochemistry-Paraffin-NB100-1063-img0008.jpg)
![Flow Cytometry: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Flow-Cytometry-NB100-1063-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1063-img0010.jpg)
![Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-71680-img0002.jpg "Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71680-img0006.jpg)
![Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71680-img0005.jpg)
![Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-71680-img0001.jpg)
![Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Coronin-1a antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71680-img0004.jpg)
![CyTOF-ready: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-CyTOF-ready-NBP2-71680-img0003.jpg)
![Western Blot: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BLNK.](http://images.novusbio.com/fullsize/BLNK-Antibody-OTI4C9-Azide-and-BSA-Free-Western-Blot-NBP2-71855-img0001.jpg "Western Blot: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BLNK.")
![Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71855-img0006.jpg)
![Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71855-img0002.jpg)
![Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71855-img0003.jpg)
![Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71855-img0004.jpg)
![Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71855-img0005.jpg)
![Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/BLNK-Antibody-OTI4C9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71855-img0007.jpg)
![Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Western-Blot-NBP1-97587-img0006.jpg "Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).")
![Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Immunohistochemistry-Paraffin-NBP1-97587-img0005.jpg)
![Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x10^5) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.](http://images.novusbio.com/fullsize/APRIL-TNFSF13-Antibody-Aprily-8-Flow-Cytometry-NBP1-97587-img0004.jpg)
![Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03; purified, GAM-APC) signal on human peripheral blood; surface staining, 6 ug/ml.](http://images.novusbio.com/fullsize/Mouse-IgM-Isotype-Control-PFR-03-NBP2-62224-img0008.jpg "Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03; purified, GAM-APC) signal on human peripheral blood; surface staining, 6 ug/ml.")
![Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03) FITC signal on human peripheral blood; surface staining, 1 ug/ml.](http://images.novusbio.com/fullsize/Mouse-IgM-Isotype-Control-PFR-03-NBP2-62224-img0009.jpg)
![Mouse IgM Isotype Control (PFR-03) [NBP2-62224] - Example of nonspecific mouse IgM (PFR-03) PE signal on human peripheral blood; surface staining, 3 ug/ml.](http://images.novusbio.com/fullsize/Mouse-IgM-Isotype-Control-PFR-03-NBP2-62224-img0010.jpg)