| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81119-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81113-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75899SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81045-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35895-100ug | |||
| Carrier-Free | NBP2-35895-500ug | |||
| Carrier-Free | NBP2-35895-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20109-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0003.jpg "Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.")
![Immunohistochemistry: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of human kidney](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-NBP2-31130-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C4d Antibody [NBP2-31130] - Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using Complement C4d antibody. Requires HIER using Tris-EDTA buffer pH 8.5 prior to staining.](http://images.novusbio.com/fullsize/Complement-C4d-Antibody-Immunohistochemistry-Paraffin-NBP2-31130-img0002.jpg)
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202492029.jpg%3Fv=1666701381 "Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).")
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of HepG2 cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-10420249141939.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202491625.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024920223.jpg%3Fv=1666701381)
![ELISA: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024916215.jpg%3Fv=1666701381)
![Western Blot: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (NBP3-12029) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/4-1BB-TNFRSF9-CD137-Antibody-4B4-1-1-Chimeric-Western-Blot-NBP3-12029-img0001.jpg "Western Blot: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Human thymus(A), lymph node(B) and tonsil(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4B4-1-1 (NBP3-12029) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 4-1BB/TNFRSF9/CD137 Antibody (4B4-1-1) - Chimeric [NBP3-12029] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 4B4-1-1 (NBP3-12029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/4-1BB-TNFRSF9-CD137-Antibody-4B4-1-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12029-img0002.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0001.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0002.jpg)
![ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-ELISA-NBP2-81119-img0001.jpg "ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.")
![Surface Plasmon Resonance: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - Surface plasmon resonance (SPR) sensorgram TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119]. 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein to immbolised the rabbit IgG chimeric version of 3B3 [NBP2-81119]. TIM-1 Fc-fusion protein was injected and allowed to associate with NBP2-81119 between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. TIM-1 Fc-fusion protein showed rapid binding to NBP2-81119 and following ligand removal remained strongly bound. This suggests that NBP2-81119 has a very high affinity for TIM-1 Fc-fusion protein.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-Surface-Plasmon-Resonance-NBP2-81119-img0002.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Western-Blot-NBP2-81113-img0002.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Flow-cytometry using the Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (NBP2-81113, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Flow-Cytometry-NBP2-81113-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric - Azide and BSA Free [NBP2-81113] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with NBP2-81113 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81113, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81113_rabbit-monoclonal-fas-tnfrsf6-cd95-antibody-r-125224-chimeric-74202522141233.png)
![Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-75899] - Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b (NBP2-75899) at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. NBP2-75899 successfully detected CD52 in mouse spleen samples.](http://images.novusbio.com/fullsize/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Western-Blot-NBP2-75899-img0001.jpg "Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-75899] - Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b (NBP2-75899) at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. NBP2-75899 successfully detected CD52 in mouse spleen samples.")
![Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Western-Blot-NBP2-81045-img0003.jpg "Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.")
![Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (NBP2-81045, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Flow-Cytometry-NBP2-81045-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-81045 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81045, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81045_rabbit-monoclonal-beta-2-microglobulin-antibody-bbm-1-chimeric-74202522113026.png)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231856948.jpg "Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: nNOS Antibody (M401) [NBP3-23224] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231820296.jpg)
![Functional: Human IL1 beta Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL1-beta-Protein-Functional-NBP2-35895-img0001.jpg "Functional: Human IL1 beta Protein [NBP2-35895]")
![SDS-Page: Human IL-1 beta/IL-1F2 Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35895-img0002.jpg)
![Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924288.png "Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-43B-8 (NBP3-20109) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20109, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924248.png)
![Flow Cytometry: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-43B-8 (NBP3-20109, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924262.png)
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)