| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75127 | |||
| Biotin | NBP1-75128 | |||
| DyLight 350 | NBP1-75948 | |||
| DyLight 405 | NBP1-75949 | |||
| DyLight 488 | NBP1-75132 | |||
| DyLight 550 | NBP1-75950 | |||
| DyLight 594 | NBP1-75951 | |||
| DyLight 633 | NBP1-75952 | |||
| DyLight 650 | NBP1-75953 | |||
| FITC | NBP1-75129 | |||
| HRP | NBP1-75130 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00319-50ul | |||
![Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntenin 1.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Western-Blot-NBP2-74418-img0002.jpg "Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntenin 1.")
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0004.jpg)
![Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Syntenin 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Western-Blot-NBP2-74418-img0001.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0003.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Human liver tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0005.jpg)
![Western Blot: CD23/Fc epsilon RII Antibody (OTI2B6) - Azide and BSA Free [NBP2-70701] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD23.](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-OTI2B6-Azide-and-BSA-Free-Western-Blot-NBP2-70701-img0001.jpg "Western Blot: CD23/Fc epsilon RII Antibody (OTI2B6) - Azide and BSA Free [NBP2-70701] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD23.")
![Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) - Azide and BSA Free [NBP2-70701] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70701-img0004.jpg)
![Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) - Azide and BSA Free [NBP2-70701] - Analysis of living 293T cells transfected with FCER2 overexpression plasmid .](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-OTI2B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70701-img0003.jpg)
![Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) - Azide and BSA Free [NBP2-70701] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70701-img0005.jpg)
![Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) - Azide and BSA Free [NBP2-70701] - Analysis of living RPMI-8226 cells, using anti-FCER2 antibody , compared to an isotype control (green), and a PBS control (blue) (1:100).](http://images.novusbio.com/fullsize/CD23-Fc-epsilon-RII-Antibody-OTI2B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70701-img0002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-75321-img0004.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-75321] - Total cell lysate from human UMUC1. This image was submitted via customer Review. Image from the HRP version of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed).")
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of paraffin-embedded human kidney tissue using anti-IL1 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunohistochemistry-Paraffin-NBP2-67865-img0004.jpg)
![Flow Cytometry: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of Hela cells with IL1 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Flow-Cytometry-NBP2-67865-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0002.jpg)
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human liver with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-2352025162812.png "Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human liver with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human epididymis with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-235202511444411.png)
![Immunoprecipitation: DCXR Antibody [NBP3-00319] - DCXR was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate 2 uL NBP3-00319 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00319, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-235202511354130.png)
![Immunohistochemistry-Paraffin: DCXR Antibody [NBP3-00319] - Immunochemical staining of human DCXR in human kidney with NBP3-00319 (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-23520251633500.png)
![Western Blot: DCXR Antibody [NBP3-00319] - NBP3-00319 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:29 kDa](http://images.novusbio.com/fullsize/nbp3-00319_rabbit-polyclonal-dcxr-antibody-23520251664778.png)
![Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Knockout-Validated-NBP1-03250-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Western blot of extracts from Human PBMC, SYK Antibody (SYK-01)](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP1-03250-img0002.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunohistochemistry-Paraffin-NBP1-03250-img0001.jpg)
![Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-288202412461675.jpg "Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32908) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29820241492635.jpg)
![Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling T-bet/TBX21 with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-292024119237.jpg)
![Flow Cytometry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Flow cytometric analysis of NK-92 cells labeling T-bet/TBX21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32908, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32908_rabbit-t-bet-tbx21-mab-je60-20-29202416453215.jpg)
![Western Blot: NUT Antibody [NBP3-32672] - Western blot analysis of NUT on mouse testis tissue lysates with Rabbit anti-NUT antibody (NBP3-32672) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-278202416331244.jpg "Western Blot: NUT Antibody [NBP3-32672] - Western blot analysis of NUT on mouse testis tissue lysates with Rabbit anti-NUT antibody (NBP3-32672) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32672) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32672) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-29820241411542.jpg)
![Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling NUT with Rabbit anti-NUT antibody (NBP3-32672) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32672, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-298202414115413.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody [NBP3-32672] - Immunocytochemistry analysis of 293T transfacted with or without mouse NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32672) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32672) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-29202410484895.jpg)
![Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-278202415212458.jpg "Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-29820241411547.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunocytochemistry analysis of HeLa cells labeling RHAMM/CD168 with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-2920241114816.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Flow cytometric analysis of HeLa cells labeling RHAMM/CD168. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32129, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32129_rabbit-rhamm-cd168-mab-psh02-16-392024884629.jpg)