| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33116 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47903-0.1mg | |||
| IHC-Prediluted | NBP2-48227 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31365SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08281 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87041-25ul | |||
![Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2682024158261.jpg "Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds")
![Western Blot: CD19 Antibody (PSH03-94) [NBP3-32130] - Western blot analysis of CD19 on mouse spleen tissue lysates with Rabbit anti-CD19 antibody (NBP3-32130) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32130) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-278202415212455.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD19 with Rabbit anti-CD19 antibody (NBP3-32130) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32130, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-29820241420976.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-CD19 antibody (NBP3-32130) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32130) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2982024149261.jpg)
![Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Western-Blot-NBP2-89121-img0001.jpg "Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Immunohistochemistry-Paraffin-NBP2-89121-img0002.jpg)
![Flow Cytometry: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Flow-Cytometry-NBP2-89121-img0003.jpg)
![Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Western-Blot-NBP3-09029-img0001.jpg "Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (NBP3-09029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09029-img0002.jpg)
![Flow Cytometry: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free [NBP3-09029] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09029 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-09029_rabbit-monoclonal-hvem-tnfrsf14-antibody-hmhv-1b18-chimeric-74202522141222.png)
![Western Blot: CD3 epsilon Antibody [NBP2-29472] - Western Blot of Jurkat cell lysate using CD3 epsilon Antibody](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Western-Blot-NBP2-29472-img0002.jpg "Western Blot: CD3 epsilon Antibody [NBP2-29472] - Western Blot of Jurkat cell lysate using CD3 epsilon Antibody")
![Western Blot: CD3 epsilon Antibody [NBP2-29472] - Analysis of CD3e in human Jurkat lysate in the 1) absence and 2) presence of immunizing peptide, 3) on mouse thymus tissue lysate probed with 2 ug/ml of CD3e antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Western-Blot-NBP2-29472-img0001.jpg)
![Western Blot: ICAM-3/CD50 Antibody (ICAM3/1019) [NBP2-44550] - Analysis of HL-60 Cell Lysate using CD50 Monoclonal Antibody (ICAM3/1019)](http://images.novusbio.com/fullsize/ICAM-3-CD50-Antibody-ICAM3-1019-Western-Blot-NBP2-44550-img0002.jpg "Western Blot: ICAM-3/CD50 Antibody (ICAM3/1019) [NBP2-44550] - Analysis of HL-60 Cell Lysate using CD50 Monoclonal Antibody (ICAM3/1019)")
![Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (ICAM3/1019) [NBP2-44550] - Human Tonsil stained with CD50 Monoclonal Antibody (ICAM3/1019)](http://images.novusbio.com/fullsize/ICAM-3-CD50-Antibody-ICAM3-1019-Immunohistochemistry-Paraffin-NBP2-44550-img0001.jpg)
![Western Blot: CD3 epsilon Antibody [NBP2-32282] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Western-Blot-NBP2-32282-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CD3 epsilon Antibody [NBP2-32282] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-32282] - Sample: FFPE section of human lung cancer infiltrate. Antibody: Affinity purified rabbit anti- CD3E used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP2-32282-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CD3 epsilon Antibody [NBP2-32282] - Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for IP at 6 ug per reaction. CD3E was also immunoprecipitated by rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (NBP2076424) and another rabbit anti-CD3E antibody. For blotting immunoprecipitated CD3E, NBP2-32282 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-Immunoprecipitation-NBP2-32282-img0005.jpg)
![Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.](http://images.novusbio.com/fullsize/CD137-Antibody-Western-Blot-NBP2-31365-img0001.jpg "Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.")
![Immunocytochemistry/Immunofluorescence: CD6 Antibody (C6/2884R) [NBP3-07555] - Immunofluorescence staining of MOLT-4 cells using CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (green). Nuclei are stained with RedDot.](http://images.novusbio.com/fullsize/CD6-Antibody-C6-2884R-Immunocytochemistry-Immunofluorescence-NBP3-07555-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD6 Antibody (C6/2884R) [NBP3-07555] - Immunofluorescence staining of MOLT-4 cells using CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (green). Nuclei are stained with RedDot.")
![Immunohistochemistry-Paraffin: CD6 Antibody (C6/2884R) [NBP3-07555] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R).](http://images.novusbio.com/fullsize/CD6-Antibody-C6-2884R-Immunohistochemistry-Paraffin-NBP3-07555-img0003.jpg)
![Flow Cytometry: CD6 Antibody (C6/2884R) [NBP3-07555] - Flow Cytometric Analysis of MOLT-4 cells. CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/CD6-Antibody-C6-2884R-Flow-Cytometry-NBP3-07555-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0004.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody [NBP1-87041] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-Immunohistochemistry-Paraffin-NBP1-87041-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NTAL Antibody [NBP1-89661] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.](http://images.novusbio.com/fullsize/NTAL-Antibody-Immunohistochemistry-Paraffin-NBP1-89661-img0002.jpg)
![Immunohistochemistry-Paraffin: NTAL Antibody [NBP1-89661] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/NTAL-Antibody-Immunohistochemistry-Paraffin-NBP1-89661-img0006.jpg)
