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![Western Blot: HVEM/TNFRSF14 Antibody (BLR062G) [NBP2-78834] - Detection of human HVEM/TNFRSF14 by WB of SK-MEL-28, A2058, UACC-257, MDA-MB-435, and Malme-3M lysate. Antibody: Rabbit anti-HVEM/TNFRSF14 recombinant monoclonal [BLR062G] (NBP2-78834). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-BLR062G-Western-Blot-NBP2-78834-img0001.jpg "Western Blot: HVEM/TNFRSF14 Antibody (BLR062G) [NBP2-78834] - Detection of human HVEM/TNFRSF14 by WB of SK-MEL-28, A2058, UACC-257, MDA-MB-435, and Malme-3M lysate. Antibody: Rabbit anti-HVEM/TNFRSF14 recombinant monoclonal [BLR062G] (NBP2-78834). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: HVEM/TNFRSF14 Antibody (BLR062G) [NBP2-78834] - Detection of human HVEM/TNFRSF14 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-HVEM/TNFRSF14 recombinant monoclonal [BLR062G] (NBP2-78834). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-BLR062G-Immunocytochemistry-NBP2-78834-img0002.jpg)
![Immunohistochemistry-Paraffin: HVEM/TNFRSF14 Antibody (BLR062G) [NBP2-78834] - Detection of human HVEM/TNFRSF14 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-HVEM/TNFRSF14 recombinant monoclonal [BLR062G] (NBP2-78834). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-BLR062G-Immunohistochemistry-NBP2-78834-img0004.jpg)
![Immunohistochemistry-Paraffin: HVEM/TNFRSF14 Antibody (BLR062G) [NBP2-78834] - Detection of human HVEM/TNFRSF14 in FFPE tonsil by IHC. Antibody: Rabbit anti-HVEM/TNFRSF14 recombinant monoclonal [BLR062G] (NBP2-78834). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-BLR062G-Immunohistochemistry-NBP2-78834-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NKAP Antibody [NBP1-82117] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NKAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82117-img0005.jpg "Immunocytochemistry/Immunofluorescence: NKAP Antibody [NBP1-82117] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NKAP Antibody [NBP1-82117] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NKAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82117-img0011.jpg)
![Immunohistochemistry-Paraffin: NKAP Antibody [NBP1-82117] - Staining of human cerebral cortex shows nuclear positivity in neurons.](http://images.novusbio.com/fullsize/NKAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82117-img0006.jpg)
![Immunohistochemistry-Paraffin: NKAP Antibody [NBP1-82117] - Staining of human tonsil shows nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/NKAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82117-img0009.jpg)
![Immunohistochemistry-Paraffin: NKAP Antibody [NBP1-82117] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NKAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82117-img0010.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Western-Blot-NBP2-76416-img0002.jpg "Western Blot: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 lysate. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 in FFPE HDLM-2 cells by ICC. Antibody: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunocytochemistry-NBP2-76416-img0001.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416) and rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417).](http://images.novusbio.com/fullsize/B7-2-CD86-Antibody-BLR029F-Immunoprecipitation-NBP2-76416-img0003.jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR029F) [NBP2-76416] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76416_rabbit-monoclonal-b7-2-cd86-antibody-blr029f-immunoprecipitation-13120251501..jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0004.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the FITC conjugate of NBP1-43416. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-Human CD40 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0003.jpg)
![Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Antibody-Western-Blot-NB300-990-img0005.jpg "Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Western-Blot-NBP3-15612-img0004.jpg "Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded human lung cancer using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0001.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded mouse spleen using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BTK-Antibody-2J9O8-Immunohistochemistry-Paraffin-NBP3-15612-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: BTK Antibody (2J9O8) [BTK] - Immunofluorescence analysis of paraffin-embedded mouse spleen using BTK Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15612_rabbit-btk-mab-2j9o8-52202517311037.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0002.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.")
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Analysis (surface staining) of human peripheral blood with anti-CD33 (WM53) purified antibody (low endotoxin), GAM-APC.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0001.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0005.jpg "Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.")
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0001.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0002.jpg)
![Immunoprecipitation: Alpha Actinin 3 Antibody [NBP2-15356] - Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 ug of alpha Actinin 3 antibody. Western blot analysis was performed using alpha Actinin 3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Immunoprecipitation-NBP2-15356-img0004.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP2-15356 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15356_rabbit-polyclonal-alpha-actinin-3-antibody-9102024205628.jpg)