| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90271-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NB500-622-100Tests | |||
| PE | NBP1-41282 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37715 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99049-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12036-0.025mg | |||
![Western Blot: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Western blot analysis of B7-H3/CD276 on different lysates with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32139) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-278202415212492.jpg "Western Blot: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Western blot analysis of B7-H3/CD276 on different lysates with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32139) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Immunocytochemistry analysis of THP-1 cells labeling B7-H3/CD276 with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-2920241048482.jpg)
![Flow Cytometry: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Flow cytometric analysis of THP-1 cells labeling B7-H3/CD276. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-392024884623.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0002.jpg "Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunochemical staining of human AIF-1/Iba1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-031-Immunohistochemistry-Paraffin-NBP2-90271-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody (031) [NBP2-90271] - Immunofluorescence staining of AIF-1/Iba1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90271 (dilution ratio 1:60) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90271_rabbit-monoclonal-aif-1-iba1-antibody-031-235202514492357.png)
![Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the FITC conjugate of NB500-403. Staining of Human peripheral blood lymphocytes with HCD99 FITC.](http://images.novusbio.com/fullsize/CD99-Antibody-MEM-131-Flow-Cytometry-NB500-403-img0004.jpg "Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the FITC conjugate of NB500-403. Staining of Human peripheral blood lymphocytes with HCD99 FITC.")
![Flow Cytometry: CD99 Antibody (MEM-131) [NB500-403] - Analysis using the Biotin conjugate of NB500-403. Staining of Human peripheral blood lymphocytes biotinylated HCD99, followed by Sav-PE.](http://images.novusbio.com/fullsize/CD99-Antibody-MEM-131-Flow-Cytometry-NB500-403-img0003.jpg)
![Western Blot: CCR8 Antibody (JE31-99) [NBP3-32117] - Western blot analysis of CCR8 on mouse thymus tissue lysates with Rabbit anti-CCR8 antibody (NBP3-32117) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 41 kDa Observed band size: 48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32117) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32117_rabbit-ccr8-mab-je31-99-278202415295512.jpg "Western Blot: CCR8 Antibody (JE31-99) [NBP3-32117] - Western blot analysis of CCR8 on mouse thymus tissue lysates with Rabbit anti-CCR8 antibody (NBP3-32117) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 41 kDa Observed band size: 48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32117) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CCR8 Antibody (JE31-99) [NBP3-32117] - Immunocytochemistry analysis of Raji cells labeling CCR8 with Rabbit anti-CCR8 antibody (NBP3-32117) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody (NBP3-32117) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32117_rabbit-ccr8-mab-je31-99-2920241192314.jpg)
![Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) PE.](http://images.novusbio.com/fullsize/CD2-Antibody-TS1-8-Flow-Cytometry-NBP2-37714-img0003.jpg "Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) PE.")
![Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) PE-CyTM7.](http://images.novusbio.com/fullsize/CD2-Antibody-TS1-8-Flow-Cytometry-NBP2-37714-img0001.jpg)
![Flow Cytometry: CD2 Antibody (TS1/8) [NBP2-37714] - Staining of CD2 in human peripheral blood with anti-CD2 (TS1/8) APC-CyTM7.](http://images.novusbio.com/fullsize/CD2-Antibody-TS1-8-Flow-Cytometry-NBP2-37714-img0002.jpg)
![Flow Cytometry: CD2 Antibody (TS1/8) - BSA Free [NBP2-37714] - Surface staining pattern of human peripheral blood stained using anti-human CD2 (TS1/8) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/nbp2-37714_mouse-monoclonal-cd2-antibody-ts1-8-17720249362615.jpg)
![Western Blot: CD35 Antibody (A4G1-R) [NBP3-32150] - Western blot analysis of CD35 on different lysates with Mouse anti-CD35 antibody (NBP3-32150) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Human kidney tissue lysate Lane 3: K-562 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 224 kDa Observed band size: 280 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32150) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32150_mouse-cd35-mab-a4g1-r-278202416285277.jpg "Western Blot: CD35 Antibody (A4G1-R) [NBP3-32150] - Western blot analysis of CD35 on different lysates with Mouse anti-CD35 antibody (NBP3-32150) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Human kidney tissue lysate Lane 3: K-562 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 224 kDa Observed band size: 280 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32150) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD35 Antibody (A4G1-R) [NBP3-32150] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD35 with Mouse anti-CD35 antibody (NBP3-32150) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32150, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32150_mouse-cd35-mab-a4g1-r-298202413585556.jpg)
![Immunohistochemistry: CD35 Antibody (A4G1-R) [NBP3-32150] - Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Mouse anti-CD35 antibody (NBP3-32150) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32150) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32150_mouse-cd35-mab-a4g1-r-298202413585599.jpg)
![Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-288202414191541.jpg "Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33110) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-29820241435257.jpg)
![Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-278202415242680.jpg "Western Blot: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Western blot analysis of IFN-gamma R1/CD119 on different lysates with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 ug/Lane) Lane 2: LoVo cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: THP-1 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (negative) (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: SW620 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse thymus tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IFN-gamma R1/CD119 Antibody (JE40-50) [NBP3-32458] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFN-gamma R1/CD119 antibody (NBP3-32458) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32458) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32458_rabbit-ifn-gamma-r1-cd119-mab-je40-50-298202414333652.jpg)
![Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-NBP2-99049-img0003.jpg "Immunohistochemistry: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: IKK alpha Antibody [NBP2-99049] - Immunochemical staining of human IKK alpha in human breast carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/IKK-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99049-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: IKK alpha Antibody [NBP2-99049] - Immunofluorescence staining of IKK alpha in HCT116 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99049 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-99049_rabbit-polyclonal-ikk-alpha-antibody-2352025166478.png)
![Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-288202412493428.jpg "Western Blot: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Western blot analysis of TGF-beta 1 on different lysates with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/2,000 dilution. Lane 1: Raji cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: Mouse spleen tissue lysate (20 ug/Lane) Lane 4: Rat spleen tissue lysate (20 ug/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TGF-beta 1 Antibody (PD00-17) [NBP3-33008] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF-beta 1 antibody (NBP3-33008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33008) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33008_rabbit-tgf-beta-1-mab-pd00-17-29820241420920.jpg)
![Immunohistochemistry-Paraffin: CCR8 Antibody [NB100-709] - Section of mouse spleen.](http://images.novusbio.com/fullsize/CCR8-Antibody-Immunohistochemistry-Paraffin-NB100-709-img0002.jpg "Immunohistochemistry-Paraffin: CCR8 Antibody [NB100-709] - Section of mouse spleen.")
![Western Blot: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 0.05 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD98-Antibody-RL388-Chimeric-Western-Blot-NBP3-12036-img0001.jpg "Western Blot: CD98 Antibody (RL388) - Chimeric [NBP3-12036] - Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (NBP3-12036) at 0.05 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: CD98 Antibody (RL388) - Chimeric - Azide and BSA Free [NBP3-12036] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with NBP3-12036 at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12036, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12036_rabbit-monoclonal-cd98-antibody-rl388-chimeric-74202522141236.png)