| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52710-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54624-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12075-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12059-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52669-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48466 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47909-0.1mg | |||
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Detection using 10 ug/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Western-Blot-NBP2-54624-img0004.jpg "Western Blot: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Detection using 10 ug/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.")
![Flow Cytometry: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM3/5 [308/3-3] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Flow-Cytometry-NBP2-54624-img0002.jpg)
![Sandwich ELISA: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM3/5 [308/3-3] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Sandwich-ELISA-NBP2-54624-img0001.jpg)
![Immunoprecipitation: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Immunoprecipitation utilizing 10 ug/ml Anti-CEACAM3/5 [308/3-3]. Detection of the sample using 5 ug/ml pAb Anti-CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Immunoprecipitation-NBP2-54624-img0003.jpg)
![Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Western-Blot-NBP3-12075-img0001.jpg "Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 84-3C1 (NBP3-12075) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12075, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12075-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free [NBP3-12075] - K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12075 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12075_rabbit-monoclonal-cd43-sialophorin-antibody-84-3c1-chimeric-74202522141237.png)
![Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Western-Blot-NBP3-09029-img0001.jpg "Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (NBP3-09029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09029-img0002.jpg)
![Flow Cytometry: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free [NBP3-09029] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09029 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-09029_rabbit-monoclonal-hvem-tnfrsf14-antibody-hmhv-1b18-chimeric-74202522141222.png)
![Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD53-Antibody-HD77-Chimeric-Western-Blot-NBP3-12059-img0001.jpg "Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12059 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12059, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12059_rabbit-monoclonal-cd53-antibody-hd77-chimeric-74202522265216.png)
![Flow Cytometry: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12059 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12059_rabbit-monoclonal-cd53-antibody-hd77-chimeric-7420252214126.png)
![Western Blot: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with NBP2-52669 at 2 ug/ml before being detected by a secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Western-Blot-NBP2-52669-img0003.jpg "Western Blot: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with NBP2-52669 at 2 ug/ml before being detected by a secondary antibody.")
![Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed rat spinal chord slices were were stained with a NBP2-52669 at 3 ug/ml.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Immunohistochemistry-NBP2-52669-img0004.jpg)
![Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed guinea pig brain slices were were stained with a NBP2-52669 at 3 ug/ml.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Immunohistochemistry-NBP2-52669-img0001.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (TFRC/1817) - Azide and BSA Free [NBP2-54467] - Formalin-fixed, paraffin-embedded Human Placenta stained](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-TFRC-1817-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54467-img0003.jpg "Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (TFRC/1817) - Azide and BSA Free [NBP2-54467] - Formalin-fixed, paraffin-embedded Human Placenta stained")
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (TFRC/1817) [NBP2-54467] - Formalin-fixed, paraffin-embedded Human Placenta stained with CD71 Monoclonal Antibody (TFRC/1817).](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-TFRC-1817-Immunohistochemistry-Paraffin-NBP2-54467-img0001.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (TFRC/1817) - Azide and BSA Free [NBP2-54467] - Formalin-fixed, paraffin-embedded Human Bone Marrow stained](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-TFRC-1817-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54467-img0002.jpg)
![Immunohistochemistry-Paraffin: CD59 Antibody (MACIF/629) - Azide and BSA Free [NBP2-47820] - Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Antibody (MACIF/629).](http://images.novusbio.com/fullsize/CD59-Antibody-MACIF-629-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47820-img0001.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (MACIF/629) - Azide and BSA Free [NBP2-47820] - Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Antibody (MACIF/629).")
![Immunohistochemistry-Paraffin: CD59 Antibody (MACIF/629) - Azide and BSA Free [NBP2-47820] - Human Tongue stained with CD59 Monoclonal Antibody (MACIF/629)](http://images.novusbio.com/fullsize/CD59-Antibody-MACIF-629-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47820-img0002.jpg)
![Western Blot: TIA1 Antibody (TIA1/1313) - BSA Free [NBP2-54406] - HepG2, PC3 and HeLa Cell Lysates with TIA1 Monoclonal Antibody (TIA1/1313).](http://images.novusbio.com/fullsize/TIA1-Antibody-TIA1-1313-BSA-Free-Western-Blot-NBP2-54406-img0001.jpg "Western Blot: TIA1 Antibody (TIA1/1313) - BSA Free [NBP2-54406] - HepG2, PC3 and HeLa Cell Lysates with TIA1 Monoclonal Antibody (TIA1/1313).")
![Immunocytochemistry/Immunofluorescence: TIA1 Antibody (TIA1/1313) - BSA Free [NBP2-54406] - Immunofluorescence Analysis of PFA-fixed HeLa cells using TIA1Mouse Monoclonal Antibody (TIA1/1313) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.](http://images.novusbio.com/fullsize/TIA1-Antibody-TIA1-1313-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54406-img0006.jpg)
![Flow (Intracellular): TIA1 Antibody (TIA1/1313) - BSA Free [NBP2-54406] - An intracellular stain was performed on A549 cells with TIA1 Antibody (TIA1/1313) NBP2-54406R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image using the BSA Free format of this antibody.](http://images.novusbio.com/fullsize/TIA1-Antibody-TIA1-1313-BSA-Free-Flow-Intracellular-NBP2-54406-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Placenta stained with CD34 MAb (QBEnd/10 + HPCA1/763).](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0005.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Placenta stained with CD34 MAb (QBEnd/10 + HPCA1/763).")
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Monoclonal Antibody (QBE.nd/10 + HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763).](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) - IHC-Prediluted [NBP2-48362] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763).](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48362-img0004.jpg)