| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24397SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33555-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64761-100ug | |||
| BSA Free | NB100-64761-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76881SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37295-0.025ml | |||
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using FOXP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Immunohistochemistry-Paraffin-NBP2-24397-img0002.jpg "Immunohistochemistry-Paraffin: FoxP3 Antibody [NBP2-24397] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using FOXP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.")
![Western Blot: FoxP3 Antibody [NBP2-24397] - Analysis of FOXP3 in (A) FOXP3 transfected and (B) mock transfected cell lysate using NBP2-24397 at 5 ug/ml.](http://images.novusbio.com/fullsize/FoxP3-Antibody-Western-Blot-NBP2-24397-img0003.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0014.jpg "Western Blot: MBP Antibody [NBP2-33555] - Analysis in mouse cerebral cortex tissue.")
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse olfactory bulb shows immunoreactivity in processes in external plexiform layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0011.jpg)
![Western Blot: MBP Antibody [NBP2-33555] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/MBP-Antibody-Western-Blot-NBP2-33555-img0013.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebral cortex shows positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0005.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human hippocampus shows moderate immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0006.jpg)
![Immunohistochemistry-Paraffin: MBP Antibody [NBP2-33555] - Staining of human cerebellum shows moderate nuclear and cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-Paraffin-NBP2-33555-img0015.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebellum shows immunoreactivity in granular cell layer and in white matter.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0007.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse cerebral cortex shows labeling of neuronal processes in motor cortex and in corpus callosum.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0008.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse dorsal tenia tecta shows positivity in neuronal processes.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0009.jpg)
![Immunohistochemistry: MBP Antibody [NBP2-33555] - Staining of mouse hippocampus shows positivity in neuronal processes in the CA1 layer.](http://images.novusbio.com/fullsize/MBP-Antibody-Immunohistochemistry-NBP2-33555-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-OX-39-Immunocytochemistry-Immunofluorescence-NB100-64761-img0004.jpg "Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power")
![Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-OX-39-Immunohistochemistry-Paraffin-NB100-64761-img0002.jpg)
![Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-OX-39-Flow-Cytometry-NB100-64761-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-OX-39-Immunocytochemistry-Immunofluorescence-NB100-64761-img0001.jpg)
![Western Blot: TRAILR3/TNFRSF10C Antibody [NBP1-76881] - Analysis of 20 ug of whole cell lysate from HeLa (Lane 1) and 293 (Lane 2) cells with anti-TRAIL-R3 at 1 ug/ml dilution. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TRAILR3-TNFRSF10C-Antibody-Western-Blot-NBP1-76881-img0002.jpg "Western Blot: TRAILR3/TNFRSF10C Antibody [NBP1-76881] - Analysis of 20 ug of whole cell lysate from HeLa (Lane 1) and 293 (Lane 2) cells with anti-TRAIL-R3 at 1 ug/ml dilution. Image submitted by a verified customer review.")
![Western Blot: TRAILR3/TNFRSF10C Antibody [NBP1-76881] - HeLa whole cell lysate with TRAIL-R3 antibody (ED) at 1:500 dilution.](http://images.novusbio.com/fullsize/TRAILR3-TNFRSF10C-Antibody-Western-Blot-NBP1-76881-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody - BSA Free [NBP1-76881] - Immunofluorescence of TRAILR3/TNFRSF10C in Hela cells with TRAILR3/TNFRSF10C antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76881_rabbit-polyclonal-trailr3-tnfrsf10c-antibody-2711202420324822.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody - BSA Free [NBP1-76881] - Immunocytochemistry of TRAILR3/TNFRSF10C in HeLa cells with TRAILR3/TNFRSF10C antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76881_rabbit-polyclonal-trailr3-tnfrsf10c-antibody-271120242074690.jpg)
![Western Blot: PD-L1 Antibody [NB300-903] - Staining of U2OS cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NB300-903-img0002.jpg "Western Blot: PD-L1 Antibody [NB300-903] - Staining of U2OS cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NB300-903] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-903-img0004.jpg)
![Flow Cytometry: PD-L1 Antibody [NB300-903] - Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Flow-Cytometry-NB300-903-img0005.jpg)
![Western Blot: PD-L1 Antibody [NB300-903] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NB300-903-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NB300-903] - Immunofluorescence analysis of paraformaldehyde fixed AA431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-903-img0003.jpg)
![Western Blot: CD14 Antibody [NB100-2807] - Staining (1ug/ml) of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD14-Antibody-Flow-Cytometry-NB100-2807-img0006.jpg "Western Blot: CD14 Antibody [NB100-2807] - Staining (1ug/ml) of PBM lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Detected by chemiluminescence.")
![Flow Cytometry: CD14 Antibody [NB100-2807] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-Flow-Cytometry-NB100-2807-img0005.jpg)
![Immunohistochemistry: Goat Polyclonal CD14 Antibody [NB100-2807] - (8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/antibody/nb100-2807_goat-polyclonal-cd14-antibody-imgenex-img-3991-immunohistochemistry-283202413831..jpg)
![Control: Goat Polyclonal CD14 Antibody [NB100-2807] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/antibody/nb100-2807_goat-polyclonal-cd14-antibody-imgenex-img-3991-control-283202413949..jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Treatment with HBOT or PFD decreased IL-17A expression in the AD lesions. IHC for IL-17A on mouse skin. Scale bar is 100 um. The number of IL-17A-expressing cells was counted in five high-power fields (HPF) chosen at random in each slide by three different pathologists. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109297) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-TC11-18H10-Immunohistochemistry-Paraffin-NBP1-72027-img0003.jpg "Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Treatment with HBOT or PFD decreased IL-17A expression in the AD lesions. IHC for IL-17A on mouse skin. Scale bar is 100 um. The number of IL-17A-expressing cells was counted in five high-power fields (HPF) chosen at random in each slide by three different pathologists. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109297) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Analysis of anti-IL-17 antibody of mouse spleen at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-TC11-18H10-Immunohistochemistry-Paraffin-NBP1-72027-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - TG2-dependent chemokine production in keratinocytes is critical for IL-17-producing CCR6+ gamma δT-cell & neutrophil dermal infiltration.a, b Effect of DMSO- or IMQ-treated WT- or TG2−/−-CM on CCR6+ gamma δT-cell (a) & CD11b+ Ly-6G+ cell (b) migration according to Transwell migration assays. Flow cytometric analysis of the percentage of cells migrating toward the CM in the presence or absence of anti-CXCL1 (a) & anti-CCL20 (b) antibodies (n = 3/group). c, d WT & TG2−/− mouse-derived CCR6+ gamma δT-cell (c) & CD11b+ Ly-6G+ cell (d) migration in response to recombinant mouse CCL20 & CXCL1, respectively. Data represent the mean ± SEM (n = 3/group). *p < 0.05; **p < 0.01. e, f Cells were isolated from the back skin of WT & TG2−/− mice treated with IMQ for four consecutive days & analyzed by flow cytometry. Representative flow cytometric profiles of CCR6+ gamma δTCRlow cells (e) & CD11bhigh Ly-6G+ cells (f). The percentage of cells is shown. Data represent the mean ± SEM (n = 9/group). g Skin sections were immunostained with CCR6 (green)- & IL-17 (red)-specific antibodies. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. h–j Back skin was separated into epidermis & dermis & analyzed by RT-PCR for IL-17-producing gamma δT-cell markers (h) (Ccr6 & Il23r), IL17 & related cytokines (i) (Il17a, Il17f, & Il22), & myeloid cell-derived cytokines (j) (Il12a & Il23a). Data represent the mean ± SEM (n = 11/group). *p < 0.05; **p < 0.01 vs. WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32355189), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-72027_rat-il-17-il-17a-mab-tc11-18h10-azide-and-bsa-free-310202416171471.jpg)
![Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Western-Blot-NBP2-37295-img0003.jpg "Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of HepG2 cells using CD14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunocytochemistry-NBP2-37295-img0008.jpg)
![Immunohistochemistry-Paraffin: CD14 Antibody (1H5D8) [NBP2-37295] - Human tonsil FFPE tissue. Antigen retrieval with EDTA buffer. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunohistochemistry-Paraffin-NBP2-37295-img0009.jpg)
![Western Blot: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Western-Blot-NBP2-37295-img0002.jpg)
![Immunohistochemistry: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of paraffin-embedded cervical cancer tissues using CD14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunohistochemistry-NBP2-37295-img0005.jpg)
![Immunohistochemistry: CD14 Antibody (1H5D8) [NBP2-37295] - Analysis of paraffin-embedded colon tissues using CD14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-Immunohistochemistry-NBP2-37295-img0007.jpg)
![ELISA: CD14 Antibody (1H5D8) [NBP2-37295] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD14-Antibody-1H5D8-ELISA-NBP2-37295-img0001.jpg)
![Western Blot: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Western-Blot-NBP2-70364-img0001.jpg "Western Blot: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.")
![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD80.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70364-img0002.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human pancreas tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0008.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0003.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0004.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0005.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human colon tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0006.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human liver tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0007.jpg)
![Immunohistochemistry: B7-1/CD80 Antibody (OTI2E5) - Azide and BSA Free [NBP2-70364] - Staining of paraffin-embedded Human prostate tissue using anti-CD80 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-OTI2E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70364-img0009.jpg)
![Western Blot: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Western blot analysis of Ubiquitin D on different lysates with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: HepG2 cell lysateLane 3: NIH/3T3 cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 18 kDaObserved band size: 20 kDaExposure time: 26 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP2-67917) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-western-blot-1312026165644.jpg "Western Blot: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Western blot analysis of Ubiquitin D on different lysates with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: HepG2 cell lysateLane 3: NIH/3T3 cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 18 kDaObserved band size: 20 kDaExposure time: 26 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP2-67917) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - ICC staining of Ubiquitin D in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP2-67917, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/FAT10-Antibody-JJ084-09-Immunocytochemistry-Immunofluorescence-NBP2-67917-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - ICC staining of Ubiquitin D in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP2-67917, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/FAT10-Antibody-JJ084-09-Immunocytochemistry-Immunofluorescence-NBP2-67917-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAT10 Antibody (JJ084-09) [NBP2-67917] - ICC staining of Ubiquitin D in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP2-67917, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/FAT10-Antibody-JJ084-09-Immunocytochemistry-Immunofluorescence-NBP2-67917-img0002.jpg)
![Immunohistochemistry: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP2-67917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-immunohistochemistry-paraffin-131202617029.jpg)
![Immunohistochemistry: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP2-67917) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-immunohistochemistry-paraffin-131202617226.jpg)
![Immunohistochemistry: Rabbit Monoclonal FAT10 Antibody (JJ084-09) [NBP2-67917] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Ubiquitin D antibody (NBP2-67917) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP2-67917) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67917_rabbit-monoclonal-fat10-antibody-jj084-09-immunohistochemistry-paraffin-131202617524.jpg)
![In vitro assay: TNFSF4 Protein [NBP2-26577] - Demonstration in vitro of the potency of Fc-OX40L in comparison to an agonist antibody OX86, with anti-CD3 alone serving as a negative control for co-stimulation.](http://images.novusbio.com/fullsize/TNFSF4-Protein-In-vitro-assay-NBP2-26577-img0001.jpg "In vitro assay: TNFSF4 Protein [NBP2-26577] - Demonstration in vitro of the potency of Fc-OX40L in comparison to an agonist antibody OX86, with anti-CD3 alone serving as a negative control for co-stimulation.")
![SDS-Page: TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-20% reducing SDS-PAGE.](http://images.novusbio.com/fullsize/TNFSF4-Protein-SDS-Page-NBP2-26577-img0002.jpg)
![SDS-Page: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-15% reducing SDS-PAGE.](http://images.novusbio.com/fullsize/Recombinant-Mouse-OX40-Ligand-TNFSF4-Protein-SDS-Page-NBP2-26577-img0004.jpg)