| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61791-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97521-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33236 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21583-50ul | |||
![Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-2120249374936.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - IFIT3 antibody [HL2468] detects IFIT3 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. IFIT3 stained by IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-212024940380.jpg)
![Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - IFIT3 antibody [HL2468] detects IFIT3 protein at cytoplasm by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 5 min. Green: IFIT3 stained by IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-2120249413353.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: IFIT3 Antibody (HL2468) - Azide and BSA Free [NBP3-25515] - Untreated (-) and treated (+) A549 whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2468] (NBP3-25515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25515_rabbit-ifit3-mab-hl2468-azide-and-bsa-free-2120249374997.jpg)
![Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-Western-Blot-NBP1-51958-img0003.jpg "Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-NBP1-51958-img0005.jpg)
![Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human tonsil. Antbody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-NBP1-51958-img0004.jpg)
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0003.jpg "Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of various cell lines, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded human placenta using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0002.jpg)
![Western Blot: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Western blot analysis of extracts of Rat liver, using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Western-Blot-NBP3-16626-img0004.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase PILS/ARTS1 Antibody (6V8C0) [NBP3-16626] - Immunohistochemistry of paraffin-embedded rat lung using Aminopeptidase PILS/ARTS1 Rabbit mAb (NBP3-16626) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aminopeptidase-PILS-ARTS1-Antibody-6V8C0-Immunohistochemistry-Paraffin-NBP3-16626-img0001.jpg)
![Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TNFAIP1-Antibody-Western-Blot-NBP2-82039-img0001.jpg "Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.")
![Immunohistochemistry: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunohistochemistry of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-271120241935386.jpg)
![Immunocytochemistry/ Immunofluorescence: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunofluorescence of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 20 ug/ml.Red: TNFAIP1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-2711202420285833.gif)
![Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against human PLXNA1 (AA: 1100-1200) recombinant protein. (Expected MW is 36.8 kDa)](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Western-Blot-NBP2-61791-img0004.jpg "Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against human PLXNA1 (AA: 1100-1200) recombinant protein. (Expected MW is 36.8 kDa)")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis of Hela cells using PLXNA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Immunocytochemistry-Immunofluorescence-NBP2-61791-img0003.jpg)
![Flow Cytometry: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis of Hela cells using PLXNA1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-ELISA-NBP2-61791-img0002.jpg)
![Western Blot: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Analysis using PLXNA1 mAb against HEK293 (1) and PLXNA1 (AA: 1100-1200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-Western-Blot-NBP2-61791-img0005.jpg)
![ELISA: Plexin A1 Antibody (8A10A6) [NBP2-61791] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Plexin-A1-Antibody-8A10A6-ELISA-NBP2-61791-img0001.jpg)
![Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Antibody-CLI-9-Western-Blot-NBP1-97521-img0002.jpg "Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.")
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)
![Western Blot: ATF2 [p Thr71] Antibody (103C411.2) [NB100-56594] - Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr.](http://images.novusbio.com/fullsize/ATF2-p-Thr71-Antibody-103C411-2-Western-Blot-NB100-56594-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF2 [p Thr71] Antibody (103C411.2) [NB100-56594] - Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr.")
![Western Blot: COX-1 Antibody (S02-6G4) [NBP3-19609] - Western blot detection of COX-1 in C6, 3T3 cell lysates using NBP3-19609 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 69kDa.](http://images.novusbio.com/fullsize/COX-1-Antibody-S02-6G4-Western-Blot-NBP3-19609-img0003.jpg "Western Blot: COX-1 Antibody (S02-6G4) [NBP3-19609] - Western blot detection of COX-1 in C6, 3T3 cell lysates using NBP3-19609 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 69kDa.")
![Immunohistochemistry-Paraffin: COX-1 Antibody (S02-6G4) [NBP3-19609] - Immunohistochemistry of COX-1 in paraffin-embedded Human colon cancer tissue using NBP3-19609 at dilution 1/5](http://images.novusbio.com/fullsize/COX-1-Antibody-S02-6G4-Immunohistochemistry-Paraffin-NBP3-19609-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (S02-6G4) [NBP3-19609] - Immunofluorescence of COX-1 (green) in Hela cells using NBP3-19609 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19609_rabbit-cox-1-mab-s02-6g4-1132025155597.jpg)
![Immunohistochemistry: c-jun [p Ser 63] Antibody (SR1842) [NBP3-21583] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21583.](http://images.novusbio.com/fullsize/nbp3-21583_rabbit-c-jun-p-ser-63-mab-sr1842-57202316112182.jpg "Immunohistochemistry: c-jun [p Ser 63] Antibody (SR1842) [NBP3-21583] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21583.")
![Western Blot: c-jun [p Ser 63] Antibody (SR1842) [NBP3-21583] - Western blot analysis using NBP3-21583 in NIH/3T3 cell lysate treated with Anisomycin.](http://images.novusbio.com/fullsize/nbp3-21583_rabbit-c-jun-p-ser-63-mab-sr1842-5720231523499.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser 63] Antibody (SR1842) [NBP3-21583] - Immunofluorescent analysis of HeLa cells treated with anisomycin, using NBP3-21583.](http://images.novusbio.com/fullsize/nbp3-21583_rabbit-c-jun-p-ser-63-mab-sr1842-6720231051269.jpg)
![Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - PSMB10 monoclonal antibody (M01), clone 3F8 Analysis of PSMB10 expression in Jurkat.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-3F8-Western-Blot-H00005699-M01-img0007.jpg "Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - PSMB10 monoclonal antibody (M01), clone 3F8 Analysis of PSMB10 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Analysis of monoclonal antibody to PSMB10 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-3F8-Immunocytochemistry-Immunofluorescence-H00005699-M01-img0005.jpg)
![Sandwich ELISA: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Detection limit for recombinant GST tagged PSMB10 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PSMB10-MECL1-Antibody-3F8-Sandwich-ELISA-H00005699-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-08854] - Immunofluorescence Analysis of PFA-fixed HeLa cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti- Mouse IgG-CF488 (green). Counterstain is phalloidin.](http://images.novusbio.com/fullsize/IRF3-Antibody-PCRP-IRF3-1E6-Immunocytochemistry-Immunofluorescence-NBP3-08854-img0002.jpg "Immunocytochemistry/Immunofluorescence: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-08854] - Immunofluorescence Analysis of PFA-fixed HeLa cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti- Mouse IgG-CF488 (green). Counterstain is phalloidin.")
![Flow Cytometry: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-08854] - Flow Cytometric Analysis of PFA-fixed HeLa cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/IRF3-Antibody-PCRP-IRF3-1E6-Flow-Cytometry-NBP3-08854-img0001.jpg)
![Protein Array: IRF3 Antibody (PCRP-IRF3-1E6) [NBP3-08854] - Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E6).](http://images.novusbio.com/fullsize/IRF3-Antibody-PCRP-IRF3-1E6-Protein-Array-NBP3-08854-img0003.jpg)