| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90097-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90037-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75195-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12048-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35105-1mg | |||
| Carrier-Free | NBP2-35105-500ug | |||
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0002.jpg "Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human tonsil with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: CD58/LFA-3 Antibody (126) [NBP2-90097] - Staining of human CD58 in human appendix with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CD58-LFA-3-Antibody-126-Immunohistochemistry-Paraffin-NBP2-90097-img0001.jpg)
![Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-278202416331267.jpg "Western Blot: CD3 Antibody (PD01-43) [NBP3-32140] - Western blot analysis of CD3 on Jurkat cell lysates with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32140) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD3 Antibody (PD01-43) [NBP3-32140] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD3 antibody (NBP3-32140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32140_mouse-cd3-mab-pd01-43-29820241492648.jpg)
![Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-278202416331259.jpg "Western Blot: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Western blot analysis of Siglec-2/CD22 on Daudi cell lysates with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32136) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Siglec-2/CD22 Antibody (PO00-31) [NBP3-32136] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Siglec-2/CD22 antibody (NBP3-32136) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32136) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32136_rabbit-siglec-2-cd22-mab-po00-31-298202414115429.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0003.jpg "Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human melanoma with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0001.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (351) [NBP2-90037] - Staining of human c-KIT in human skin with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-351-Immunohistochemistry-Paraffin-NBP2-90037-img0002.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Surface staining pattern of human peripheral blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.")
![Flow Cytometry: Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - Separation of human CD22 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD22 (S-HCL-1) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-S-HCL-1-Flow-Cytometry-NBP3-09167-img0002.jpg)
![Western Blot: Mouse Monoclonal Siglec-2/CD22 Antibody (S-HCL-1) [NBP3-09167] - The reactivity of S-HCL-1 antibody was assessed by comparing binding signals in a panel of three human B cell and one T cell line. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Ramos, Daudi, and Jurkat cell lines, mixed and heated (100C, 5 min) without reducing agent in SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG2b monoclonal antibody S-HCL-1 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).CD22 was detected at ~110-125 kDa in Daudi cell line.](http://images.novusbio.com/fullsize/antibody/nbp3-09167_mouse-monoclonal-siglec-2-cd22-antibody-s-hcl-1-western-blot-11122024122650..jpg)
![Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-288202412493457.jpg "Western Blot: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Western blot analysis of Thrombopoietin R/Tpo R on different lysates with Rabbit anti-Thrombopoietin R/Tpo R antibody (NBP3-33039) at 1/1,000 dilution. Lane 1: Mouse placenta tissue lysate (40 ug/Lane) Lane 2: NIH/3T3 cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (no heat) (20 ug/Lane) Lane 4: Rat spleen tissue lysate (40 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 71 kDa Observed band size: 80 kDa Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Thrombopoietin R/Tpo R Antibody (JE58-00) [NBP3-33039] - Flow cytometric analysis of A431 cells labeling Thrombopoietin R/Tpo R. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33039, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33039_rabbit-thrombopoietin-r-tpo-r-mab-je58-00-29202416372192.jpg)
![Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-298202414161868.jpg "Immunohistochemistry: CD47 Antibody (PD00-33) [NBP3-32156] - Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (NBP3-32156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: CD47 Antibody (PD00-33) [NBP3-32156] - Flow cytometric analysis of Jurkat cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32156, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32156_rabbit-cd47-mab-pd00-33-392024884610.jpg)
![Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-278202416285213.jpg "Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-2920241192322.jpg)
![Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-Flow-Cytometry-NBP2-75195-img0002.jpg "Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody")
![ELISA: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Binding curve of anti-B7-H3 antibody MJ18 (NBP2-75195) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-75195. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-ELISA-NBP2-75195-img0001.jpg)
![Immunocytochemistry /Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with NBP3-12048 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12048_rabbit-monoclonal-marco-antibody-plk1-chimeric-74202522113028.png "Immunocytochemistry /Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with NBP3-12048 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![SDS-Page: Mouse IL-1ra/IL-1F3/IL1RN Protein [NBP2-35105]](http://images.novusbio.com/fullsize/Mouse-IL-1ra-IL-1F3-IL1RN-Protein-SDS-Page-NBP2-35105-img0001.jpg "SDS-Page: Mouse IL-1ra/IL-1F3/IL1RN Protein [NBP2-35105]")