| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11970-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52669-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54624-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52710-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12075-0.025mg | |||
![Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 ug/Lane) Lane 2: NCI-H226 cell lysate (20 ug/Lane) Lane 3: Human brain tissue lysate (30 ug/Lane) Lane 4: Human placenta tissue lysate (30 ug/Lane) Lane 5: Human lung tissue lysate (30 ug/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32132) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-278202415295558.jpg "Western Blot: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Western blot analysis of CD200/OX2 on different lysates with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 ug/Lane) Lane 2: NCI-H226 cell lysate (20 ug/Lane) Lane 3: Human brain tissue lysate (30 ug/Lane) Lane 4: Human placenta tissue lysate (30 ug/Lane) Lane 5: Human lung tissue lysate (30 ug/Lane) Predicted band size: 31 kDa Observed band size: 40-50kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32132) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD200/OX2 with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32132, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414333642.jpg)
![Immunohistochemistry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200/OX2 antibody (NBP3-32132) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-298202414304113.jpg)
![Flow Cytometry: CD200/OX2 Antibody (PSH01-48) [NBP3-32132] - Flow cytometric analysis of SK-MEL-28 cells labeling CD200/OX2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32132, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32132_rabbit-cd200-ox2-mab-psh01-48-29202416453219.jpg)
![Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-288202412442915.jpg "Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32887) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29820241420957.jpg)
![Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887] - Immunocytochemistry analysis of SH-SY5Y cells labeling Rac1,2,3 with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29202410234241.jpg)
![Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Western-Blot-NBP2-89121-img0001.jpg "Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Immunohistochemistry-Paraffin-NBP2-89121-img0002.jpg)
![Flow Cytometry: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Flow-Cytometry-NBP2-89121-img0003.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0005.jpg "Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R))488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Flow-Cytometry-NBP2-66896-img0004.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: Raji cell lysateLane 2: Daudi cell lysate](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0001.jpg)
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue)](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0002.jpg)
![Western Blot: CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric [NBP3-11970] - Mouse thymus (A) and mouse brain (B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (NBP3-11970) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCRL2-CRAM-A-B-Antibody-BZ5B8-Chimeric-Western-Blot-NBP3-11970-img0001.jpg "Western Blot: CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric [NBP3-11970] - Mouse thymus (A) and mouse brain (B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (NBP3-11970) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CCRL2/CRAM-A/B Antibody (BZ5B8) - Chimeric [NBP3-11970] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BZ5B8 (NBP3-11970) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11970, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCRL2-CRAM-A-B-Antibody-BZ5B8-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11970-img0002.jpg)
![Flow Cytometry: Rabbit Monoclonal CCRL2/CRAM-A/B Antibody (BZ5B8) [NBP3-11970] - Flow cytometry analysis of RAW264.7 cells.](http://images.novusbio.com/fullsize/nbp3-11970_rabbit-monoclonal-ccrl2-cram-a-b-antibody-bz5b8-chimeric-flow-cytometry-1922025154542..png)
![Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Western-Blot-NBP3-09029-img0001.jpg "Western Blot: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (NBP3-09029) at 0.01 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric [NBP3-09029] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (NBP3-09029) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09029, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/HVEM-TNFRSF14-Antibody-HMHV-1B18-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09029-img0002.jpg)
![Flow Cytometry: HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free [NBP3-09029] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09029 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-09029_rabbit-monoclonal-hvem-tnfrsf14-antibody-hmhv-1b18-chimeric-74202522141222.png)
![Western Blot: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with NBP2-52669 at 2 ug/ml before being detected by a secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Western-Blot-NBP2-52669-img0003.jpg "Western Blot: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with NBP2-52669 at 2 ug/ml before being detected by a secondary antibody.")
![Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed rat spinal chord slices were were stained with a NBP2-52669 at 3 ug/ml.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Immunohistochemistry-NBP2-52669-img0004.jpg)
![Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed guinea pig brain slices were were stained with a NBP2-52669 at 3 ug/ml.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-735-Chimeric-Immunohistochemistry-NBP2-52669-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP3-23369] - Immunocytochemical labeling of actin-related protein 2/3 complex subunit 1B in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B (C-terminal region) (NBP3-23369) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23369_rabbit-actr3-pab-1992023191548.jpg "Immunocytochemistry/Immunofluorescence: ACTR3 Antibody [NBP3-23369] - Immunocytochemical labeling of actin-related protein 2/3 complex subunit 1B in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B (C-terminal region) (NBP3-23369) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: ACTR3 Antibody [NBP3-23369] - Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-actin-related protein 2/3 complex subunit 1B (C-terminal region) antibody at 1:1000 (lanes 1-3).](http://images.novusbio.com/fullsize/nbp3-23369_rabbit-actr3-pab-199202312403719.jpg)
![Western Blot: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Detection using 10 ug/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Western-Blot-NBP2-54624-img0004.jpg "Western Blot: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Detection using 10 ug/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.")
![Flow Cytometry: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM3/5 [308/3-3] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Flow-Cytometry-NBP2-54624-img0002.jpg)
![Sandwich ELISA: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM3/5 [308/3-3] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Sandwich-ELISA-NBP2-54624-img0001.jpg)
![Immunoprecipitation: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Immunoprecipitation utilizing 10 ug/ml Anti-CEACAM3/5 [308/3-3]. Detection of the sample using 5 ug/ml pAb Anti-CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.](http://images.novusbio.com/fullsize/CEACAM3-5-Antibody-308-3-3-Immunoprecipitation-NBP2-54624-img0003.jpg)
![Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Western-Blot-NBP3-12075-img0001.jpg "Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 84-3C1 (NBP3-12075) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12075, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12075-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free [NBP3-12075] - K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12075 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12075_rabbit-monoclonal-cd43-sialophorin-antibody-84-3c1-chimeric-74202522141237.png)