| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74731 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72517 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16925-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93240-0.02ml | |||
![Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ERK2-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18632-img0001.jpg "Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0001.jpg)
![Western Blot: UbcH5a/UBE2D1 Antibody (1E7) [NBP2-46269] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D1 .](http://images.novusbio.com/fullsize/UbcH5a-UBE2D1-Antibody-1E7-Western-Blot-NBP2-46269-img0001.jpg "Western Blot: UbcH5a/UBE2D1 Antibody (1E7) [NBP2-46269] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D1 .")
![Western Blot: Lnx1 Antibody (1H9) [NBP2-45794] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lnx1.](http://images.novusbio.com/fullsize/Lnx1-Antibody-1H9-Western-Blot-NBP2-45794-img0001.jpg "Western Blot: Lnx1 Antibody (1H9) [NBP2-45794] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lnx1.")
![ELISA: Mouse Hemopexin ELISA Kit (Colorimetric) [NBP2-60633] - Standard curve.](http://images.novusbio.com/fullsize/Mouse-Hemopexin-ELISA-Kit-Colorimetric-ELISA-NBP2-60633-img0001.jpg "ELISA: Mouse Hemopexin ELISA Kit (Colorimetric) [NBP2-60633] - Standard curve.")
![Western Blot: COX1 Antibody [NBP1-62466] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/COX1-Antibody-Western-Blot-NBP1-62466-img0002.jpg "Western Blot: COX1 Antibody [NBP1-62466] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: HspA4 Antibody [NBP1-54348] - Human Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/HspA4-Antibody-Western-Blot-NBP1-54348-img0004.jpg "Western Blot: HspA4 Antibody [NBP1-54348] - Human Brain lysate, concentration 0.2-1 ug/ml.")
![Western Blot: HspA4 Antibody [NBP1-54348] - Tissue: Human 721_B.](http://images.novusbio.com/fullsize/HspA4-Antibody-Western-Blot-NBP1-54348-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB [p Ser142] Antibody [NBP3-05412] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CREB antibody (NBP3-05412). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CREB [p Ser142] Antibody-Immunohistochemistry-Paraffin-NBP3-05412-img0001.jpg "Immunohistochemistry-Paraffin: CREB [p Ser142] Antibody [NBP3-05412] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CREB antibody (NBP3-05412). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/TSC2-[p-Ser1387]-Antibody-Western-Blot-NBP3-16925-img0001.jpg "Western Blot: TSC2 [p Ser1387] Antibody [NBP3-16925] - Western blot analysis of extracts of C2C12 cells, using TSC2 Rabbit pAb (NBP3-16925) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.")
![SDS-Page: Recombinant Human Glutamine Synthetase Protein [NBP2-52376] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Recombinant-Protein-SDS-Page-NBP2-52376-img0001.jpg "SDS-Page: Recombinant Human Glutamine Synthetase Protein [NBP2-52376] - 15% SDS Page (3 ug)")
![Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05462_rabbit-polyclonal-ampk-alpha-1-p-ser485-antibody-52202516334154.jpg "Western Blot: AMPK alpha 1 [p Ser485] Antibody - Azide and BSA Free [AMPK alpha 1] - Western blot analysis of lysates from C6 cells using AMPK alpha 1 Rabbit pAb at 1:2000 dilution. C6 cells were treated by lambdapp(20uL/400ul) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Western Blot: PARP12 Antibody [NBP2-93240] - Analysis of extracts of various cell lines, using PARP12 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/PARP12-Antibody-Western-Blot-NBP2-93240-img0001.jpg "Western Blot: PARP12 Antibody [NBP2-93240] - Analysis of extracts of various cell lines, using PARP12 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")