Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-262202415355711.jpg "Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-272202485637.jpg)
![Western Blot: PARP Antibody (7C11) [NBP3-26438] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-282202411472110.jpg)
![Western Blot: Endo G Antibody [NBP2-16331] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP2-16331-img0002.jpg "Western Blot: Endo G Antibody [NBP2-16331] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:500.")
![Western Blot: Endo G Antibody [NBP2-16331] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Endo-G-Antibody-Western-Blot-NBP2-16331-img0001.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-2722024847194.jpg "Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.")
![Western Blot: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Positive Western Blot detected in: SH-SY5Y whole cell lysate. All lanes: AKT1/2/3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa Observed band size: 56 kDa](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-282202411442636.jpg)
![Flow Cytometry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Overlay Peak curve showing Jurkat cells stained with AKT1/2/3 Antibody (16E10) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-28220241418367.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F8) [NBP3-15146] - Immunofluorescence staining of Hela cells with at HSP90 antibody 1:29, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/HSP90-Antibody-4F8-Immunocytochemistry-Immunofluorescence-NBP3-15146-img0001.jpg "Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F8) [NBP3-15146] - Immunofluorescence staining of Hela cells with at HSP90 antibody 1:29, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).")
![Immunohistochemistry: HSP90 Antibody (4F8) [NBP3-15146] - IHC image of HSP90 diluted at 1:87.5 and staining in paraffin-embedded human endometrial cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F8-Immunohistochemistry-NBP3-15146-img0002.jpg)
![Immunohistochemistry: HSP90 Antibody (4F8) [NBP3-15146] - IHC image of HSP90 antibody diluted at 1:87.5 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F8-Immunohistochemistry-NBP3-15146-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (ADFP/1366) [NBP2-53405] - Formalin-fixed, paraffin-embedded human Adrenal stained with Adipophilin Monoclonal Antibody (ADFP/1366).](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1366-Immunohistochemistry-Paraffin-NBP2-53405-img0001.jpg "Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (ADFP/1366) [NBP2-53405] - Formalin-fixed, paraffin-embedded human Adrenal stained with Adipophilin Monoclonal Antibody (ADFP/1366).")
![Protein Array: Perilipin-2/ADFP Antibody (ADFP/1366) [NBP2-53405] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Perilipin-2/ADFP Antibody (ADFP/1366). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Perilipin-2-ADFP-Antibody-ADFP-1366-Protein-Array-NBP2-53405-img0002.jpg)
![Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NB100-766-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: RPTOR Antibody [NB100-766] - Detection of human Raptor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Raptor antibody NB100-766 (lot 3) used for IP at 6 ug per reaction. Raptor was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-Raptor antibody NB100-331 For blotting immunoprecipitated Raptor, NB100-766 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunoprecipitation-NB100-766-img0005.jpg)
![Western Blot: PCAF Antibody [NBP1-31192] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE PCAF antibody [C3], C-term dilution: 1:1000](http://images.novusbio.com/fullsize/PCAF-Antibody-Western-Blot-NBP1-31192-img0005.jpg "Western Blot: PCAF Antibody [NBP1-31192] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE PCAF antibody [C3], C-term dilution: 1:1000")
![Western Blot: PCAF Antibody [NBP1-31192] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PCAF-Antibody-Western-Blot-NBP1-31192-img0003.jpg)
![Western Blot: PCAF Antibody [NBP1-31192] - HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term diluted at 1:1000.](http://images.novusbio.com/fullsize/PCAF-Antibody-Western-Blot-NBP1-31192-img0004.jpg)