| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00261-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89676-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99356-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97279-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90677-50ul | |||
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP3-00261] - NBP3-00261 at 1:500 dilution Lane A: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa](http://images.novusbio.com/fullsize/nbp3-00261_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-235202511494046.png "Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP3-00261] - NBP3-00261 at 1:500 dilution Lane A: MOLT4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP3-00261] - Immunochemical staining of human Sodium Potassium ATPase Beta 1 in human colon carcinoma with NBP3-00261 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00261_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-23520251633508.png)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP3-00261] - Immunochemical staining of human Sodium Potassium ATPase Beta 1 in human liver with NBP3-00261 (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00261_rabbit-polyclonal-sodium-potassium-atpase-beta-1-antibody-23520251437139.png)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.Performed under reducing conditions. Predicted band size:57 kDaObserved band size:57 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-16-Western-Blot-NBP2-89676-img0003.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.Performed under reducing conditions. Predicted band size:57 kDaObserved band size:57 kDa (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 uL anti-P4HB rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-P4HB rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 57 kDa Observed band size: 57 kDa](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-16-Immunoprecipitation-NBP2-89676-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (16) [NBP2-89676] - Staining of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89676 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.](http://images.novusbio.com/fullsize/nbp2-89676_rabbit-monoclonal-protein-disulfide-isomerase-p4hb-antibody-016-235202516335016.png)
![Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Western-Blot-NBP2-99356-img0003.jpg "Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-99356] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-99356-img0002.jpg)
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-99356] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with NBP2-99356 at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-99356_rabbit-polyclonal-fih-1-hif-1an-antibody-23520252135149.png)
![Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution.<br/><br/> Lane 1: Mouse brain tissue lysate (20 ug/Lane)<br/> Lane 2: Rat brain tissue lysate (20 ug/Lane)<br/> Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane)<br/><br/> Predicted band size: 62 kDa<br/> Observed band size: 62 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-278202415242637.jpg "Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution.<br/><br/> Lane 1: Mouse brain tissue lysate (20 ug/Lane)<br/> Lane 2: Rat brain tissue lysate (20 ug/Lane)<br/> Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane)<br/><br/> Predicted band size: 62 kDa<br/> Observed band size: 62 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32287) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-29820241435275.jpg)
![Immunohistochemistry-Paraffin: OS9 Antibody [NBP2-97279] - Staining of human OS9 in human pancreas with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunohistochemistry-Paraffin-NBP2-97279-img0003.jpg "Immunohistochemistry-Paraffin: OS9 Antibody [NBP2-97279] - Staining of human OS9 in human pancreas with rabbit polyclonal antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: OS9 Antibody [NBP2-97279] - Staining of human OS9 in human placenta with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunohistochemistry-Paraffin-NBP2-97279-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: OS9 Antibody [NBP2-97279] - Immunofluorescence staining of OS9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97279 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97279_rabbit-polyclonal-os9-antibody-235202521451795.png)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0004.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0003.jpg)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input)<br/> Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input)<br/> Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 3 minutes")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Western blot analysis on different lysates with NBP3-32697 at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32697 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_-western-blot-639204597484663792.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32387) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32387_mouse-glutamine-synthetase-mab-a3g2-r-298202414333698.jpg "Immunohistochemistry: Glutamine Synthetase Antibody (A3G2-R) [NBP3-32387] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Glutamine Synthetase antibody (NBP3-32387) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32387) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32387 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: HEK-293 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32387 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32387_-western-blot-639204590076823988.jpg)
![Western Blot: S100B Antibody (PD00-11) [NBP3-32924] - Western blot analysis of S100B on SK-MEL-28 cell lysates with Rabbit anti-S100B antibody (NBP3-32924) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 11 kDa<br/> Observed band size: 11 kDa<br/><br/> Exposure time: 1 minute; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32924) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32924_mouse-s100b-mab-pd00-11-288202412442930.jpg "Western Blot: S100B Antibody (PD00-11) [NBP3-32924] - Western blot analysis of S100B on SK-MEL-28 cell lysates with Rabbit anti-S100B antibody (NBP3-32924) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 11 kDa<br/> Observed band size: 11 kDa<br/><br/> Exposure time: 1 minute; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32924) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: S100B Antibody (PD00-11) [NBP3-32924] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Mouse anti-S100B antibody (NBP3-32924) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32924) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32924_mouse-s100b-mab-pd00-11-298202414115432.jpg)
![Western blot analysis on different lysates with NBP3-32924 at 1/2,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: A-172 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse brain tissue lysate (no heat) Lane 5: Rat brain tissue lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32924 at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32924_-western-blot-639204590073764336.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Western-Blot-NBP1-44894-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0003.jpg)
![Western Blot: Myosin heavy chain 11 Antibody [NBP2-59221] - Nuclear extracts of ME-1 cells were analysed by Western blot using the antibodies against CBFb and MYH11 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Western-Blot-NBP2-59221-img0001.jpg "Western Blot: Myosin heavy chain 11 Antibody [NBP2-59221] - Nuclear extracts of ME-1 cells were analysed by Western blot using the antibodies against CBFb and MYH11 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.")
![Chromatin Immunoprecipitation: Myosin heavy chain 11 Antibody [NBP2-59221] - ChIP assays were performed using ME-1 cells, the antibody against MYH11 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Chromatin-Immunoprecipitation-NBP2-59221-img0003.jpg)
![ELISA: Myosin heavy chain 11 Antibody [NBP2-59221] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MYH11. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-ELISA-NBP2-59221-img0002.jpg)
![Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Western-Blot-NBP1-30128-img0001.jpg "Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (B20-U) [NBP1-30128] - Expression in HEK293 cells.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Immunocytochemistry-Immunofluorescence-NBP1-30128-img0005.jpg)