| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98812-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90677-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89443-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52685-0.025mg | |||
![Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-278202415242637.jpg "Western Blot: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Western blot analysis of EAAT2/GLT1 on different lysates with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 ug/Lane) Lane 2: Rat brain tissue lysate (20 ug/Lane) Lane 3: Mouse cerebellum tissue lysate (20 ug/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32287) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EAAT2/GLT1 Antibody (PS01-62) [NBP3-32287] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EAAT2/GLT1 antibody (NBP3-32287) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32287) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32287_rabbit-eaat2-glt1-mab-ps01-62-29820241435275.jpg)
![Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Western-Blot-NBP1-44894-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0002.jpg)
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0003.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-288202412461681.jpg "Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Immunocytochemistry analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202410484836.jpg)
![Flow Cytometry: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Flow cytometric analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32760, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202416453235.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-98812-img0003.jpg "Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human lung cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-98812-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP2-98812] - Immunofluorescence staining of JNK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98812 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98812_rabbit-polyclonal-jnk2-antibody-235202511545667.png)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0004.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0003.jpg)
![Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-278202415212411.jpg "Western Blot: p53 Antibody (6B5-R) [NBP3-32698] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32698) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32698) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg)
![Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-26820241582614.jpg "Immunoprecipitation: p53 Antibody (PSH03-95) [NBP3-32697] - p53 was immunoprecipitated in 0.2mg NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate with NBP3-32697 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32697 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate (input) Lane 2: NBP3-32697 IP in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Lane 3: Rabbit IgG instead of NBP3-32697 in NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes")
![Western Blot: p53 Antibody (PSH03-95) [NBP3-32697] - Western blot analysis of p53 on different lysates with Rabbit anti-p53 antibody (NBP3-32697) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5uM doxorubicincell for 24 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32697) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-278202415212448.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (PSH03-95) [NBP3-32697] - Immunocytochemistry analysis of NIH/3T3 cells treated with 0.5μM doxorubicin for 20 hours labeling p53 with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p53 antibody (NBP3-32697) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32697_rabbit-p53-mab-psh03-95-2920241048481.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0003.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)")
![Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443] - Analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 ug,) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Flow-Cytometry-NBP2-89443-img0001.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89443-img0002.jpg)
![Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Western-Blot-NBP1-30128-img0001.jpg "Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (B20-U) [NBP1-30128] - Expression in HEK293 cells.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-B20-U-Immunocytochemistry-Immunofluorescence-NBP1-30128-img0005.jpg)
![Western Blot: eNOS [p Tyr657] Antibody [NBP3-23185] - Western blot analysis of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-eNOS monoclonal antibody (NBP3-23209; lanes 1 & 2), anti-eNOS (Tyr-657) phospho-specific antibody (NP4031; lanes 3 & 4), or anti-eNOS polyclonal antibody (NBP3-23210; lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23185_rabbit-enos-p-tyr657-pab-119202316331612.jpg "Western Blot: eNOS [p Tyr657] Antibody [NBP3-23185] - Western blot analysis of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-eNOS monoclonal antibody (NBP3-23209; lanes 1 & 2), anti-eNOS (Tyr-657) phospho-specific antibody (NP4031; lanes 3 & 4), or anti-eNOS polyclonal antibody (NBP3-23210; lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: eNOS [p Tyr657] Antibody [NBP3-23185] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23185_rabbit-enos-p-tyr657-pab-19920231410130.jpg)
![Western Blot: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23246_mouse-caveolin-1-p-tyr14-mab-m283-19920231856942.jpg "Western Blot: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: Caveolin-1 [p Tyr14] Antibody (M283) [NBP3-23246] - Immunocytochemical labeling of caveolin-1 phosphorylation in rabbit spleen fibroblasts. The cells were treated with pervanadate (1 mM) for 30 min, then fixed with paraformaldehyde and labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 (Tyr-14) antibodies. The antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23246_mouse-caveolin-1-p-tyr14-mab-m283-199202312523624.jpg)
![Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/EGFR-Antibody-Matuzumab-Chimeric-Flow-Cytometry-NBP2-52685-img0002.jpg "Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (Matuzumab) - Chimeric - Azide and BSA Free [NBP2-52685] - Immunofluorescence staining of fixed A431 cells with anti-EGFR antibody Matuzumab. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP2-52685 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52685, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52685_rabbit-monoclonal-egfr-antibody-matuzumab-chimeric-74202522141213.png)