| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06611SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91937-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44478 | |||
| BSA Free | NBP2-36779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88775-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72149 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48051 | |||
| Azide and BSA Free | NBP3-11500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24698SS | |||
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: ATOX1 Antibody [NBP1-06611] - HeLa whole cell extract.](http://images.novusbio.com/fullsize/ATOX1-Antibody-Western-Blot-NBP1-06611-img0005.jpg "Western Blot: ATOX1 Antibody [NBP1-06611] - HeLa whole cell extract.")
![Immunocytochemistry/Immunofluorescence: ATOX1 Antibody [NBP1-06611] - ATOX1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ATOX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06611-img0001.jpg)
![Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Proprotein-Convertase-1-PCSK1-Antibody-3D2-Western-Blot-H00005122-M02-img0016.jpg "Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in Raw 264.7.")
![Immunocytochemistry/Immunofluorescence: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/Proprotein-Convertase-1-PCSK1-Antibody-3D2-Immunocytochemistry-Immunofluorescence-H00005122-M02-img0010.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Proprotein-Convertase-1-PCSK1-Antibody-3D2-Immunohistochemistry-Paraffin-H00005122-M02-img0012.jpg)
![Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2 Analysis of PCSK1 expression in HeLa.](http://images.novusbio.com/fullsize/Proprotein-Convertase-1-PCSK1-Antibody-3D2-Western-Blot-H00005122-M02-img0014.jpg)
![Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in PC-12.](http://images.novusbio.com/fullsize/Proprotein-Convertase-1-PCSK1-Antibody-3D2-Western-Blot-H00005122-M02-img0015.jpg)
![Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Proprotein-Convertase-1-PCSK1-Antibody-3D2-Western-Blot-H00005122-M02-img0017.jpg)
![Sandwich ELISA: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Detection limit for recombinant GST tagged PCSK1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Proprotein-Convertase-1-PCSK1-Antibody-3D2-Sandwich-ELISA-H00005122-M02-img0013.jpg)
![Western Blot: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of GOLM1 expression in transfected 293T cell line by GOLM1 monoclonal antibody (M06), clone 5B10. Lane 1: GOLM1 transfected lysatE (45.3 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-Western-Blot-H00051280-M06-img0005.jpg "Western Blot: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of GOLM1 expression in transfected 293T cell line by GOLM1 monoclonal antibody (M06), clone 5B10. Lane 1: GOLM1 transfected lysatE (45.3 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of monoclonal antibody to GOLM1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-Immunocytochemistry-Immunofluorescence-H00051280-M06-img0003.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody (5B10) [H00051280-M06] - Analysis of monoclonal antibody to GOLM1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-Immunohistochemistry-Paraffin-H00051280-M06-img0004.jpg)
![ELISA: GOLM1 Antibody (5B10) [H00051280-M06] - Detection limit for recombinant GST tagged GOLPH2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GOLM1-Antibody-5B10-ELISA-H00051280-M06-img0002.jpg)
![Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SEC23IP-Antibody-Western-Blot-NBP1-82456-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: SEC23IP Antibody [NBP1-82456] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SEC23IP-Antibody-Western-Blot-NBP1-82456-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SEC23IP Antibody [NBP1-82456] - Staining of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SEC23IP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82456-img0006.jpg)
![Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human tonsil shows moderate granular cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/SEC23IP-Antibody-Immunohistochemistry-Paraffin-NBP1-82456-img0017.jpg)
![Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SEC23IP-Antibody-Immunohistochemistry-Paraffin-NBP1-82456-img0014.jpg)
![Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SEC23IP-Antibody-Immunohistochemistry-Paraffin-NBP1-82456-img0015.jpg)
![Immunohistochemistry-Paraffin: SEC23IP Antibody [NBP1-82456] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SEC23IP-Antibody-Immunohistochemistry-Paraffin-NBP1-82456-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91937-img0005.jpg "Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0007.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human colon.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0008.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human kidney.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0009.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human epididymis.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0010.jpg)
![Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779] - analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Western-Blot-NBP2-36779-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779] - analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in U2OS cell line with Anti-GORASP2 monoclonal antibody, showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunocytochemistry-Immunofluorescence-NBP2-36779-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in human epididymis and skeletal muscle tissues. Corresponding GORASP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunohistochemistry-Paraffin-NBP2-36779-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in HeLa cell line showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunocytochemistry-Immunofluorescence-NBP2-36779-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human cell line A431 showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunocytochemistry-Immunofluorescence-NBP2-36779-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in MCF7 cell line showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunocytochemistry-Immunofluorescence-NBP2-36779-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in U251 cell line showing specific staining of the golgi apparatus in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunocytochemistry-Immunofluorescence-NBP2-36779-img0009.jpg)
![Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human endometrium shows moderate to strong membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunohistochemistry-Paraffin-NBP2-36779-img0014.jpg)
![Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human epididymis shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunohistochemistry-Paraffin-NBP2-36779-img0015.jpg)
![Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunohistochemistry-Paraffin-NBP2-36779-img0016.jpg)
![Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2610-Immunohistochemistry-Paraffin-NBP2-36779-img0017.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining in human stomach and skeletal muscle tissues using anti-GOLM1 antibody. Corresponding GOLM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88775-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining in human stomach and skeletal muscle tissues using anti-GOLM1 antibody. Corresponding GOLM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GOLM1 Antibody [NBP1-88775] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Western-Blot-NBP1-88775-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-88775] - Staining of human cell line A-431 shows positivity in golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88775-img0004.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88775-img0008.jpg)
![Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88775] - Staining of human stomach shows high expression.](http://images.novusbio.com/fullsize/GOLM1-Antibody-Immunohistochemistry-Paraffin-NBP1-88775-img0009.jpg)
![Western Blot: ACBD3 Antibody (3A1) [NBP2-03720] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Western-Blot-NBP2-03720-img0013.jpg "Western Blot: ACBD3 Antibody (3A1) [NBP2-03720] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Human prostate tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0012.jpg)
![Flow Cytometry: ACBD3 Antibody (3A1) [NBP2-03720] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACBD3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Flow-Cytometry-NBP2-03720-img0001.jpg)
![Western Blot: ACBD3 Antibody (3A1) [NBP2-03720] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACBD3.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Western-Blot-NBP2-03720-img0002.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0003.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0004.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0005.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0006.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0007.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Human breast tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0008.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Human colon tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0009.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Human liver tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0010.jpg)
![Immunohistochemistry-Paraffin: ACBD3 Antibody (3A1) [NBP2-03720] - Staining of paraffin-embedded Human pancreas tissue using anti-ACBD3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACBD3-Antibody-3A1-Immunohistochemistry-Paraffin-NBP2-03720-img0011.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Immunohistochemistry-Paraffin-NBP2-44690-img0001.jpg "Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).")
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using gp96/HSP90B1/GRP94 Antibody (SPM249).followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Flow-Cytometry-NBP2-44690-img0002.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Classical swine fever virus (CSFV) infection induces the activation of UPR. (A) RNA extracted from CSFV-infected cells was quantified for the expression of UPR genes Xbp1(s), GRP78, GRP94, EDEM-1, ATF4, ATF6, CHOP, Calreticulin, Calnexin, & ERp57 using q-PCR. Mock-infected PK-15 & Thapsigargin (TG)-treated PK-15 were used as negative & positive controls, respectively, & the fold induction was calculated compared to mock cells at the same time point. Error bars represent the mean ± SD of 3 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. (B,D,E) Immunoblotting analysis of components of UPR signaling pathways during a time course of CSFV infection. Mock or CSFV-infected PK-15 cells lysates were collected at the indicated time points. Lysates were analyzed for the activation of the IRE1 (B), PERK (D) & ATF6 (E) pathway by immunoblotting analysis. Tubulin was used as a loading control, & infection was confirmed by detecting the viral protein Npro. Results of a representative experiment of 2 independent experiments are shown. (C) RNA was collected as described above, & the splicing levels of XBP1 were analyzed with semi-quantitative PCR as described in materials & methods. The length of Xbp1(u) is 474 bp & Xbp1(s) is 448 bp. (F) The relative expression ratios of the targeted proteins/genes were analyzed by densitometric scanning. Error bars represent the mean ± SD of 2 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fmicb.2017.02129/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44690_rat-monoclonal-gp96-hsp90b1-grp94-antibody-spm249-31020241612223.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of human GRP94 in 3T3 cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24698 at 1 ug/ml.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP2-24698-img0006.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of human GRP94 in 3T3 cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24698 at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - GPR94 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24698-img0004.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of Human Placenta using NBP2-24698 at 10 ug/ml.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP2-24698-img0005.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)