| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61760-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61681-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61753-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61843-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61761-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB600-836-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24726SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91654-25ul | |||
![Western Blot: NPY5R Antibody [NB100-1538] - Staining of Human Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Western-Blot-NB100-1538-img0002.jpg "Western Blot: NPY5R Antibody [NB100-1538] - Staining of Human Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NPY5R Antibody [NB100-1538] - Staining of Human Intestine. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NPY5R-Antibody-Immunohistochemistry-Paraffin-NB100-1538-img0001.jpg)
![Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of various cell lines, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PAR2-Antibody-9X8E6-Western-Blot-NBP3-16555-img0002.jpg "Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of various cell lines, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of Rat large intestine, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PAR2-Antibody-9X8E6-Western-Blot-NBP3-16555-img0003.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202511365142.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-5220251265325.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202511361293.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-5220251138377.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202517213570.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-5220251727358.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human pancreatic cancer tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202517213531.jpg)
![Western Blot: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Analysis using P2RY4 mAb against human P2RY4 (AA: extra mix) recombinant protein. (Expected MW is 10.1 kDa)](http://images.novusbio.com/fullsize/P2Y4-P2RY4-Antibody-3B4H6-Western-Blot-NBP2-61760-img0006.jpg "Western Blot: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Analysis using P2RY4 mAb against human P2RY4 (AA: extra mix) recombinant protein. (Expected MW is 10.1 kDa)")
![Immunocytochemistry/Immunofluorescence: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Analysis of Hela cells using P2RY4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/P2Y4-P2RY4-Antibody-3B4H6-Immunocytochemistry-Immunofluorescence-NBP2-61760-img0003.jpg)
![Immunohistochemistry: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Analysis of paraffin-embedded stomach cancer tissues using P2RY4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y4-P2RY4-Antibody-3B4H6-Immunohistochemistry-NBP2-61760-img0005.jpg)
![Flow Cytometry: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Analysis of Hela cells using P2RY4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/P2Y4-P2RY4-Antibody-3B4H6-Flow-Cytometry-NBP2-61760-img0002.jpg)
![Western Blot: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Analysis using P2RY4 mAb against HEK293 (1) and P2RY4 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/P2Y4-P2RY4-Antibody-3B4H6-Western-Blot-NBP2-61760-img0007.jpg)
![Immunohistochemistry: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Analysis of paraffin-embedded cervical cancer tissues using P2RY4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y4-P2RY4-Antibody-3B4H6-Immunohistochemistry-NBP2-61760-img0004.jpg)
![ELISA: P2Y4/P2RY4 Antibody (3B4H6) [NBP2-61760] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/P2Y4-P2RY4-Antibody-3B4H6-ELISA-NBP2-61760-img0001.jpg)
![Western Blot: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis using CD183 mAb against HEK293 (1) and CD183 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-Western-Blot-NBP2-61681-img0006.jpg "Western Blot: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis using CD183 mAb against HEK293 (1) and CD183 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of paraffin-embedded esophageal cancer tissues using CD183 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-Immunohistochemistry-NBP2-61681-img0004.jpg)
![Flow Cytometry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of Ramos cells using CD183 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-Flow-Cytometry-NBP2-61681-img0008.jpg)
![Western Blot: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis using CD183 mAb against human CD183 (AA: extra mix) recombinant protein. (Expected MW is 38.3 kDa)](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-Western-Blot-NBP2-61681-img0005.jpg)
![Western Blot: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis using CD183 mouse mAb against Hela (1) and L-02 (2) cell lysate.](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-Western-Blot-NBP2-61681-img0007.jpg)
![Immunohistochemistry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of paraffin-embedded cervical cancer tissues using CD183 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-Immunohistochemistry-NBP2-61681-img0003.jpg)
![Flow Cytometry: CXCR3 Antibody (5C10E6) [NBP2-61681] - Analysis of Jurkat cells using CD183 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-Flow-Cytometry-NBP2-61681-img0002.jpg)
![ELISA: CXCR3 Antibody (5C10E6) [NBP2-61681] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CXCR3-Antibody-5C10E6-ELISA-NBP2-61681-img0001.jpg)
![Western Blot: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis using P2RY14 mAb against human P2RY14 (AA: extra mix) recombinant protein. (Expected MW is 11.5 kDa)](http://images.novusbio.com/fullsize/P2Y14-GPR105-Antibody-5A3F1-Western-Blot-NBP2-61753-img0006.jpg "Western Blot: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis using P2RY14 mAb against human P2RY14 (AA: extra mix) recombinant protein. (Expected MW is 11.5 kDa)")
![Immunocytochemistry/Immunofluorescence: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of Hela cells using P2RY14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/P2Y14-GPR105-Antibody-5A3F1-Immunocytochemistry-Immunofluorescence-NBP2-61753-img0003.jpg)
![Immunohistochemistry: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of paraffin-embedded rectum cancer tissues using P2RY14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y14-GPR105-Antibody-5A3F1-Immunohistochemistry-NBP2-61753-img0005.jpg)
![Flow Cytometry: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of Hela cells using P2RY14 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/P2Y14-GPR105-Antibody-5A3F1-Flow-Cytometry-NBP2-61753-img0002.jpg)
![Western Blot: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis using P2RY14 mAb against HEK293 (1) and P2RY14 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/P2Y14-GPR105-Antibody-5A3F1-Western-Blot-NBP2-61753-img0007.jpg)
![Immunohistochemistry: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Analysis of paraffin-embedded lung cancer tissues using P2RY14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y14-GPR105-Antibody-5A3F1-Immunohistochemistry-NBP2-61753-img0004.jpg)
![ELISA: P2Y14/GPR105 Antibody (5A3F1) [NBP2-61753] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/P2Y14-GPR105-Antibody-5A3F1-ELISA-NBP2-61753-img0001.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0005.jpg "Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of Hela cells using GRM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunocytochemistry-Immunofluorescence-NBP2-61843-img0003.jpg)
![Immunohistochemistry-Paraffin: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of cervical cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Immunohistochemistry-NBP2-61843-img0004.jpg)
![Flow Cytometry: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0002.jpg)
![Western Blot: mGluR3 Antibody (7A5A6) [NBP2-61843] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-Western-Blot-NBP2-61843-img0006.jpg)
![ELISA: mGluR3 Antibody (7A5A6) [NBP2-61843] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-7A5A6-ELISA-NBP2-61843-img0001.jpg)
![Western Blot: P2Y8 Antibody (1G5A11) [NBP2-61761] - Analysis using P2RY8 mAb against human P2RY8 (AA: extra mix) recombinant protein. (Expected MW is 6.6 kDa)](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A11-Western-Blot-NBP2-61761-img0006.jpg "Western Blot: P2Y8 Antibody (1G5A11) [NBP2-61761] - Analysis using P2RY8 mAb against human P2RY8 (AA: extra mix) recombinant protein. (Expected MW is 6.6 kDa)")
![Immunocytochemistry/Immunofluorescence: P2Y8 Antibody (1G5A11) [NBP2-61761] - Analysis of Hela cells using P2RY8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A11-Immunocytochemistry-Immunofluorescence-NBP2-61761-img0003.jpg)
![Immunohistochemistry: P2Y8 Antibody (1G5A11) [NBP2-61761] - Analysis of paraffin-embedded brain tissues using P2RY8 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A11-Immunohistochemistry-NBP2-61761-img0005.jpg)
![Flow Cytometry: P2Y8 Antibody (1G5A11) [NBP2-61761] - Analysis of Hela cells using P2RY8 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A11-ELISA-NBP2-61761-img0002.jpg)
![Western Blot: P2Y8 Antibody (1G5A11) [NBP2-61761] - Analysis using P2RY8 mAb against HEK293 (1) and P2RY8 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A11-Western-Blot-NBP2-61761-img0007.jpg)
![Immunohistochemistry: P2Y8 Antibody (1G5A11) [NBP2-61761] - Analysis of paraffin-embedded cervical cancer tissues using P2RY8 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A11-Immunohistochemistry-NBP2-61761-img0004.jpg)
![ELISA: P2Y8 Antibody (1G5A11) [NBP2-61761] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/P2Y8-Antibody-1G5A11-ELISA-NBP2-61761-img0001.jpg)
![Western Blot: EDNRA/Endothelin R Type A Antibody [NB600-836] - EDN 1 Receptor A in 1 MES Cells. Western Provided by verified customer review.](http://images.novusbio.com/fullsize/EDNRA-Endothelin-R-Type-A-Antibody-Western-Blot-NB600-836-img0006.jpg "Western Blot: EDNRA/Endothelin R Type A Antibody [NB600-836] - EDN 1 Receptor A in 1 MES Cells. Western Provided by verified customer review.")
![Immunocytochemistry/Immunofluorescence: EDNRA/Endothelin R Type A Antibody [NB600-836] - EDN 1 Receptor A in 1 MES Cells. Immunofluorescent staining image provided by verified customer review.](http://images.novusbio.com/fullsize/EDNRA-Endothelin-R-Type-A-Antibody-Immunocytochemistry-Immunofluorescence-NB600-836-img0004.jpg)
![Western Blot: EDNRA/Endothelin R Type A Antibody [NB600-836] - ET-1 A & B Receptor Western provided by verified customer review.](http://images.novusbio.com/fullsize/EDNRA-Endothelin-R-Type-A-Antibody-Western-Blot-NB600-836-img0005.jpg)
![Western Blot: TRHR Antibody [NBP2-24726] - Analysis of human TRH-R in human brain lysate in the 1) absence and 2) presence of immunizing peptide at 0.5 ug/ml.](http://images.novusbio.com/fullsize/TRHR-Antibody-Western-Blot-NBP2-24726-img0004.jpg "Western Blot: TRHR Antibody [NBP2-24726] - Analysis of human TRH-R in human brain lysate in the 1) absence and 2) presence of immunizing peptide at 0.5 ug/ml.")
![Immunohistochemistry-Paraffin: TRHR Antibody [NBP2-24726] - Staining of human thyroid using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TRHR-Antibody-Immunohistochemistry-Paraffin-NBP2-24726-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunocytochemistry-Immunofluorescence-NBP3-11866-img0005.jpg "Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11866. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunohistochemistry-Paraffin-NBP3-11866-img0002.jpg)
![Flow Cytometry: mGluR5 Antibody (ST51-00) [NBP3-11866] - Flow cytometric analysis of SH-SY-5Y cells with NBP3-11866 at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Flow-Cytometry-NBP3-11866-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunocytochemistry-Immunofluorescence-NBP3-11866-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunocytochemistry staining mGluR5 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunocytochemistry-Immunofluorescence-NBP3-11866-img0004.jpg)
![Immunohistochemistry-Paraffin: mGluR5 Antibody (ST51-00) [NBP3-11866] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using NBP3-11866. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/mGluR5-Antibody-ST51-00-Immunohistochemistry-Paraffin-NBP3-11866-img0001.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TVRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TVRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: GPR50 Antibody [H00009248-B01P] - Analysis of negative control 293 cells (Black) and GPR50 expressing 293 cells (Green) using GPR50 purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/GPR50-Antibody-Flow-Cytometry-H00009248-B01P-img0003.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - Analysis of GPR50 expression in transfected 293T cell line by GPR50 polyclonal antibody. Lane 1: GPR50 transfected lysate(67.87 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 promotes ligand-independent activation of TBRI signaling. Detection of p-Smad3, p-Stat-3, p-Jak2 (c) and p-Smad2 (d) in lysates of hypothalamus and cortex of wt and GPR50 ko mice. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 competes with FKBP12 for the binding to TBRI. Competition between FKBP12 and GPR50 for TBRI binding was checked by precipitating FKBP12 from total brain and lung lysates of wt and GPR50ko mice and revealed with anti-TBRI. Total lysate was addressed for expression of FKBP12, TBRI, and GPR50 with corresponding antibodies. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR50-Antibody-Western-Blot-H00009248-B01P-img0006.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009248-b01p_mouse-polyclonal-gpr50-antibody-310202415482257.jpg)
![Western Blot: GPR50 Antibody [H00009248-B01P] - GPR50 phosphorylate & activate T beta RI independently of T beta RII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGF beta (2 ng/mL) & pretreated or not overnight with SB431542 at 10 µM. p-Smad3 & total Smad3 levels & expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 & T beta RI in lysates of SNU638 cells expressing HA-T beta RI alone or together with GPR50 delta 4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGF beta (2 ng/mL, 1 h). Total T beta RI was immunoprecipitated with anti-Flag antibody & immunoblotted for anti-T beta RIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of T beta RI precipitation in cell lysates. d Phospho-Smad3 & Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGF beta (2 ng/mL, 1 h) & expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor & the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03609-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009248-b01p_mouse-polyclonal-gpr50-antibody-310202415482280.jpg)
![Immunocytochemistry/Immunofluorescence: AGTRAP Antibody [NBP1-91654] - Staining of human cell line A-431 shows positivity in vesicles.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91654-img0005.jpg "Immunocytochemistry/Immunofluorescence: AGTRAP Antibody [NBP1-91654] - Staining of human cell line A-431 shows positivity in vesicles.")
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0010.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0002.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Analysis in human epididymis and skeletal muscle tissues using NBP1-91654 antibody. Corresponding AGTRAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0006.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Epididymis shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0007.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0008.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0009.jpg)
![Immunohistochemistry-Paraffin: AGTRAP Antibody [NBP1-91654] - Staining of human Testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/AGTRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-91654-img0011.jpg)