| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB600-1005-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB19553-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-61026-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93587-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65601-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47909-0.1mg | |||
| IHC-Prediluted | NBP2-48362 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38322-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05772SS | |||
![Immunohistochemistry: LYVE-1 Antibody [NB600-1005] - LYVE1 Antibody [NB600-1005] - Staining of human skin showing positivity in lymphatic vessels. Image provided by Patricia Redondo.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Immunohistochemistry-NB600-1005-img0011.jpg "Immunohistochemistry: LYVE-1 Antibody [NB600-1005] - LYVE1 Antibody [NB600-1005] - Staining of human skin showing positivity in lymphatic vessels. Image provided by Patricia Redondo.")
![Flow Cytometry: LYVE-1 Antibody [NB600-1005] - Analysis with primary human dermal microvascular endothelial cells (HDMVEC).](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Flow-Cytometry-NB600-1005-img0010.jpg)
![Western Blot: LYVE-1 Antibody [NB600-1005] - Analysis of recombinant human sLYVE-1 and mouse sLYVE-1 using an anti-human LYVE-1 polyclonal antibody directed against the extracellular domain of human LYVE-1. There is more or less no cross reactivity withmouse LYVE-1.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Western-Blot-NB600-1005-img0009.jpg)
![Western Blot: CD36 Antibody (877302) [MAB19553] - Analysis of human platelets tissue lysate with 1 ug/ml of hCD36 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hCD36 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-877302-Western-Blot-MAB19553-img0002.jpg "Western Blot: CD36 Antibody (877302) [MAB19553] - Analysis of human platelets tissue lysate with 1 ug/ml of hCD36 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. A specific band was detected for hCD36 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: CD36 Antibody (877302) [MAB19553] - CD36 was detected in immersion fixed U937 cell using CD36 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-877302-Immunocytochemistry-MAB19553-img0001.jpg)
![Immunohistochemistry: CD36 Antibody (877302) [MAB19553] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using CD36 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cardiomyocytes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-877302-Immunohistochemistry-MAB19553-img0003.jpg)
![Immunohistochemistry: LYVE-1 Antibody (RM0033-4D17) [NB110-61026] - a-d As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale=30 um. n=3 brains. e-h E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale=30 um. n=3 brains. i-l E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic (j, red arrows) in the meninges but nuclear in neurons (j, blue arrows). DAPI marks the nuclei in blue (l). Scale=30 um. n=3 brains. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-019-02091-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-RM0033-4D17-Immunohistochemistry-NB110-61026-img0004.jpg "Immunohistochemistry: LYVE-1 Antibody (RM0033-4D17) [NB110-61026] - a-d As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale=30 um. n=3 brains. e-h E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale=30 um. n=3 brains. i-l E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic (j, red arrows) in the meninges but nuclear in neurons (j, blue arrows). DAPI marks the nuclei in blue (l). Scale=30 um. n=3 brains. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-019-02091-z), licensed under a CC-BY license.")
![Immunohistochemistry: LYVE-1 Antibody (RM0033-4D17) [NB110-61026] - Cells of human meninges co-express LLEC markers. DAB-IHC with single antibody detecting LYVE1 (b), in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07), and confirmed in n = 2 additional samples. P parenchyma. Scale = 40 um. DAB-IHC with single antibody detecting LYVE1 (c), in elderly human meninges (age: 89-92) with evidence of neuropathology and confirmed in n = 3 brains. P, parenchyma. Scale = 20 um. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-019-02091-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-RM0033-4D17-Immunohistochemistry-NB110-61026-img0005.jpg)
![Immunohistochemistry-Paraffin: LYVE1 Antibody (RM0033-4D17) [NB110-61026] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse LYVE-1.](http://images.novusbio.com/fullsize/LYVE1-Antibody-RM0033-4D17-Immunohistochemistry-Paraffin-NB110-61026-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-31020241534313.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye & A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A anterior, P posterior, D dorsal, V ventral. b Coronal brain section indicating the areas imaged. SF4 refers to area captured in Figure S4. c The percentage of each labelled cell type that internalized perfused A beta . Cells co-expressing VEGFR3 & LYVE1 take up A beta at a higher rate than MRC1, LYVE1 double-positive cells as well as MRC1-positive, LYVE1-negative cells (p ≤ 0.05, bootstrap). VEGFR3, LYVE1 counts, n = 2 brains (3 sections/brain). MRC1, LYVE1 counts, n = 3 brains (3 sections/brain). d–d′′′ Cells of the adult mouse meninges that co-express VEGFR3 (d, green) & LYVE1 (d′, white) internalize A beta 1-40 (d′′, cyan). Scale = 20 µm. e-e′′′) Cells of the adult mouse meninges that co-express VEGFR3 (e, green) & MRC1 (e′, white) internalize A beta 1-40 (e′′, cyan). Scale = 40 µm. f–f′′′) Cells of the adult mouse meninges that co-express MRC1 (f, magenta) & LYVE1 (f′, white) internalize A beta 1-40 (f′′, cyan). The walls of a blood vessel (white arrowhead, f′′) also accumulate A beta 1-40. Scale = 60 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-31020241621825.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old Tg(kdr-l:mCherry); Tg(flt4:mCitrine) double transgenic zebrafish has cells in the meninges (white bracket) that express flt4/vegfr3 ( alpha -GFP, green) near kdr-l positive ( alpha -RFP, red) blood vessels. DAPI (blue) labels the nuclei. Scale = 50 µm. c Coronal mouse brain section showing the imaging areas of the meninges. d As revealed by IHC, 17-week-old mouse brains express VEGFR3 (green) in the meninges (white bracket). Tie2-GFP;NG2-DsRed double reporter mice were used to distinguish arteries & veins. NG2 (red) labels pericytes & smooth muscle cells, Tie2 (magenta) labels vascular endothelial cells, & Hoechst (blue) stains nuclei. The image is rotated with the parenchyma at the bottom for ease of comparison with panel b. Scale = 50 µm. e-e′′′ As revealed by IHC, cells of the meninges co-express MRC1 (e, yellow), LYVE1 (e′, white), & VEGFR3 (e′′, green). Red arrows highlight cells expressing these three markers. The images are rotated with the parenchyma at the bottom. scale = 30 µm. f, g Quantification of the relative numbers of single & double-labelled cells in 2-month old mouse meninges. VEGFR3 & LYVE1 cell counts were from n = 2 brains, 3 coronal sections (10 area images)/brain. MRC1 & LYVE1 cell counts were from n = 3 brains, 3 coronal sections (4 area images)/brain. The mean values for each set are depicted Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-310202416203723.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), & MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), & confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); & 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), & MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology & confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), & VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) & images are merged in (k, p). Scale = 10 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-310202416205169.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. a–d As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). DAPI marks the nuclei in blue (d). Scale = 30 µm. n = 3 brains. e–h E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). DAPI marks the nuclei in blue (h). Scale = 30 µm. n = 3 brains. i–l E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). PROX1 is cytoplasmic (j, red arrows) in the meninges but nuclear in neurons (j, blue arrows). DAPI marks the nuclei in blue (l). Scale = 30 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-310202416205126.jpg)
![Western Blot: PER2 Antibody [NBP2-93587] - Analysis of extracts of 293T cells, using PER2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NBP2-93587-img0002.jpg "Western Blot: PER2 Antibody [NBP2-93587] - Analysis of extracts of 293T cells, using PER2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-93587] - Mouse testis using PER2 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-93587-img0005.jpg)
![Chromatin Immunoprecipitation: PER2 Antibody [NBP2-93587] - Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using PER2 antibody (NBP2-93587) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/PER2-Antibody-Chromatin-Immunoprecipitation-NBP2-93587-img0006.jpg)
![Western Blot: PER2 Antibody [NBP2-93587] - Analysis of extracts of Mouse liver, using PER2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PER2-Antibody-Western-Blot-NBP2-93587-img0001.jpg)
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-93587] - Rat spleen using PER2 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-93587-img0003.jpg)
![Immunohistochemistry-Paraffin: PER2 Antibody [NBP2-93587] - Human thyroid cancer using PER2 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PER2-Antibody-Immunohistochemistry-Paraffin-NBP2-93587-img0004.jpg)
![Immunohistochemistry-Frozen: Rabbit Polyclonal PER2 Antibody [NBP2-93587] - 10X magnification. Immunoreactive cell bodies (DAB staining) along the 4th ventricle in 20um from Hyla cinerea. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-93587_rabbit-polyclonal-per2-antibody-immunohistochemistry-frozen-510202311487..png)
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (SN6) NB100-65601PE and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0008.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (SN6) NB100-65601PE and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0002.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105RPE](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0004.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0001.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Analysis using the FITC conjugate of NB100-65601. Staining of KG1 cells.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (SN6) NB100-65601PCP (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0007.jpg)
![Western Blot: Hemopexin Antibody (1M6O3) [NBP3-16762] - Western blot analysis of extracts of Mouse lung, using Hemopexin (HPX) Rabbit mAb (NBP3-16762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2min.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-1M6O3-Western-Blot-NBP3-16762-img0005.jpg "Western Blot: Hemopexin Antibody (1M6O3) [NBP3-16762] - Western blot analysis of extracts of Mouse lung, using Hemopexin (HPX) Rabbit mAb (NBP3-16762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2min.")
![Western Blot: Hemopexin Antibody (1M6O3) [NBP3-16762] - Western blot analysis of extracts of Human serum, using Hemopexin (HPX) Rabbit mAb (NBP3-16762) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-1M6O3-Western-Blot-NBP3-16762-img0006.jpg)
![Immunohistochemistry-Paraffin: Hemopexin Antibody (1M6O3) [NBP3-16762] - Immunohistochemistry of paraffin-embedded rat liver using Hemopexin (HPX) Rabbit mAb (NBP3-16762) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Hemopexin-Antibody-1M6O3-Immunohistochemistry-Paraffin-NBP3-16762-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [NBP3-16762] - Confocal imaging of paraffin-embedded Rat liver using Hemopexin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16762_rabbit-hemopexin-mab-1m6o3-5220251139221.jpg)
![Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [NBP3-16762] - Confocal imaging of paraffin-embedded Human liver using Hemopexin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16762_rabbit-hemopexin-mab-1m6o3-522025125554.jpg)
![Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [Hemopexin] - Confocal imaging of paraffin-embedded Rat liver using Hemopexin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16762_rabbit-hemopexin-mab-1m6o3-52202517361279.jpg)
![Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [Hemopexin] - Confocal imaging of paraffin-embedded Human liver using Hemopexin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16762_rabbit-hemopexin-mab-1m6o3-52202517311053.jpg)
![Western Blot: LOX Antibody (9K10Z3) [NBP3-15396] - Western blot analysis of extracts of various cell lines, using LOX Rabbit mAb (NBP3-15396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Western-Blot-NBP3-15396-img0003.jpg "Western Blot: LOX Antibody (9K10Z3) [NBP3-15396] - Western blot analysis of extracts of various cell lines, using LOX Rabbit mAb (NBP3-15396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: LOX Antibody (9K10Z3) [NBP3-15396] - Immunohistochemistry of paraffin-embedded human colon using LOX Rabbit mAb (NBP3-15396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Immunohistochemistry-Paraffin-NBP3-15396-img0002.jpg)
![Immunohistochemistry-Paraffin: LOX Antibody (9K10Z3) [NBP3-15396] - Immunohistochemistry of paraffin-embedded rat kidney using LOX Rabbit mAb (NBP3-15396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Immunohistochemistry-Paraffin-NBP3-15396-img0001.jpg)
![Immunoprecipitation: LOX Antibody (9K10Z3) [NBP3-15396] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug LOX antibody (NBP3-15396). Western blot was performed from the immunoprecipitate using LOX antibody (NBP3-15396) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/LOX-Antibody-9K10Z3-Immunoprecipitation-NBP3-15396-img0004.jpg)
![Immunohistochemistry: LOX Antibody (9K10Z3) [LOX] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using LOX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15396_rabbit-lox-mab-9k10z3-52202517162390.jpg)
![Immunohistochemistry: LOX Antibody (9K10Z3) [LOX] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using LOX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15396_rabbit-lox-mab-9k10z3-52202517192984.jpg)
![Immunohistochemistry: LOX Antibody (9K10Z3) [LOX] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using LOX Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15396_rabbit-lox-mab-9k10z3-52202517202979.jpg)
![Immunohistochemistry: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Representative images of RCC patient biopsies immunostained for VEGFR2, CD34, and NRP1 and counterstained using Hoechst33342. Left column shows examples of NRP1 expressed by endothelial cells; middle column shows a tumor sample with low NRP1 expression. Right column shows NRP1 positive perivascular tumor cells. Blood vessels were stained for CD34 and nuclei counterstained using Hoechst33342. Left column shows cis complexes, localized within the endothelium. Middle column shows a tumor lacking VEGFR2/NRP1 complexes. Right column shows trans complexes, located adjacent to the endothelium (white arrowheads). Image collected and cropped by Citeab from the following publication (Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. J Pathol (2020) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-Immunohistochemistry-NBP2-44567-img0005.jpg "Immunohistochemistry: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Representative images of RCC patient biopsies immunostained for VEGFR2, CD34, and NRP1 and counterstained using Hoechst33342. Left column shows examples of NRP1 expressed by endothelial cells; middle column shows a tumor sample with low NRP1 expression. Right column shows NRP1 positive perivascular tumor cells. Blood vessels were stained for CD34 and nuclei counterstained using Hoechst33342. Left column shows cis complexes, localized within the endothelium. Middle column shows a tumor lacking VEGFR2/NRP1 complexes. Right column shows trans complexes, located adjacent to the endothelium (white arrowheads). Image collected and cropped by Citeab from the following publication (Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma. J Pathol (2020) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44567-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Human Colon Carcinoma stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44567-img0002.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Human Placenta stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44567-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10 + HPCA1/763) [NBP2-44567] - Human Tonsil stained with CD34 Monoclonal Antibody (QBEnd/10 + HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-+-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44567-img0004.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet in VEGFA induced HUVEC cell lysate (50 ug) using NB100-529 (4 ug/mL). ECL detection 2.5 minutes.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-Western-Blot-NB100-529-img0010.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet in VEGFA induced HUVEC cell lysate (50 ug) using NB100-529 (4 ug/mL). ECL detection 2.5 minutes.")
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - The VEGF R2/KDR/Flk-1antibody was tested in V6.5 mouse embryonic stem cells (NBP1-41162) with DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained with DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-529-img0008.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF Receptor 2 chimera transfected lysate (20 ug) using NB100-529 (6 ug/mL). ECL detection 30 seconds.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-Western-Blot-NB100-529-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38322] - Analysis in human placenta and cerebral cortex tissues. Corresponding CD34 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38322-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38322] - Analysis in human placenta and cerebral cortex tissues. Corresponding CD34 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD34 Antibody [NBP2-38322] - Staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38322-img0002.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38322] - Staining of human kidney shows moderate membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38322-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38322] - Staining of human placenta shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38322-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38322] - Staining of human tonsil shows moderate to strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38322-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody [NBP2-38322] - Staining of human cerebral cortex shows moderate membranous positivity in endothelial cells and very weak cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/CD34-Antibody-Immunohistochemistry-Paraffin-NBP2-38322-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NBP3-05772] - HepG2 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-VEGFR2/KDR at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05772-img0002.jpg "Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NBP3-05772] - HepG2 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-VEGFR2/KDR at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody [NBP3-05772] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of VEGFR2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05772-img0001.jpg)