| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-61026-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77241-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89844-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43861 | |||
| BSA Free | NBP2-61619-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43939 | |||
| BSA Free | NBP2-52893-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44092 | |||
| BSA Free | NBP2-59053-25ul | |||
![Western Blot: EEA1 Antibody [NBP1-05962] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05963.](http://images.novusbio.com/fullsize/EEA1-Antibody-Western-Blot-NBP1-05962-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: EEA1 Antibody [NBP1-05962] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05963.")
![Immunohistochemistry: LYVE-1 Antibody (RM0033-4D17) [NB110-61026] - a-d As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale=30 um. n=3 brains. e-h E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale=30 um. n=3 brains. i-l E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic (j, red arrows) in the meninges but nuclear in neurons (j, blue arrows). DAPI marks the nuclei in blue (l). Scale=30 um. n=3 brains. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-019-02091-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-RM0033-4D17-Immunohistochemistry-NB110-61026-img0004.jpg "Immunohistochemistry: LYVE-1 Antibody (RM0033-4D17) [NB110-61026] - a-d As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale=30 um. n=3 brains. e-h E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale=30 um. n=3 brains. i-l E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic (j, red arrows) in the meninges but nuclear in neurons (j, blue arrows). DAPI marks the nuclei in blue (l). Scale=30 um. n=3 brains. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-019-02091-z), licensed under a CC-BY license.")
![Immunohistochemistry: LYVE-1 Antibody (RM0033-4D17) [NB110-61026] - Cells of human meninges co-express LLEC markers. DAB-IHC with single antibody detecting LYVE1 (b), in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07), and confirmed in n = 2 additional samples. P parenchyma. Scale = 40 um. DAB-IHC with single antibody detecting LYVE1 (c), in elderly human meninges (age: 89-92) with evidence of neuropathology and confirmed in n = 3 brains. P, parenchyma. Scale = 20 um. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-019-02091-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-RM0033-4D17-Immunohistochemistry-NB110-61026-img0005.jpg)
![Immunohistochemistry-Paraffin: LYVE1 Antibody (RM0033-4D17) [NB110-61026] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse LYVE-1.](http://images.novusbio.com/fullsize/LYVE1-Antibody-RM0033-4D17-Immunohistochemistry-Paraffin-NB110-61026-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-31020241534313.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye & A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A anterior, P posterior, D dorsal, V ventral. b Coronal brain section indicating the areas imaged. SF4 refers to area captured in Figure S4. c The percentage of each labelled cell type that internalized perfused A beta . Cells co-expressing VEGFR3 & LYVE1 take up A beta at a higher rate than MRC1, LYVE1 double-positive cells as well as MRC1-positive, LYVE1-negative cells (p ≤ 0.05, bootstrap). VEGFR3, LYVE1 counts, n = 2 brains (3 sections/brain). MRC1, LYVE1 counts, n = 3 brains (3 sections/brain). d–d′′′ Cells of the adult mouse meninges that co-express VEGFR3 (d, green) & LYVE1 (d′, white) internalize A beta 1-40 (d′′, cyan). Scale = 20 µm. e-e′′′) Cells of the adult mouse meninges that co-express VEGFR3 (e, green) & MRC1 (e′, white) internalize A beta 1-40 (e′′, cyan). Scale = 40 µm. f–f′′′) Cells of the adult mouse meninges that co-express MRC1 (f, magenta) & LYVE1 (f′, white) internalize A beta 1-40 (f′′, cyan). The walls of a blood vessel (white arrowhead, f′′) also accumulate A beta 1-40. Scale = 60 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-31020241621825.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old Tg(kdr-l:mCherry); Tg(flt4:mCitrine) double transgenic zebrafish has cells in the meninges (white bracket) that express flt4/vegfr3 ( alpha -GFP, green) near kdr-l positive ( alpha -RFP, red) blood vessels. DAPI (blue) labels the nuclei. Scale = 50 µm. c Coronal mouse brain section showing the imaging areas of the meninges. d As revealed by IHC, 17-week-old mouse brains express VEGFR3 (green) in the meninges (white bracket). Tie2-GFP;NG2-DsRed double reporter mice were used to distinguish arteries & veins. NG2 (red) labels pericytes & smooth muscle cells, Tie2 (magenta) labels vascular endothelial cells, & Hoechst (blue) stains nuclei. The image is rotated with the parenchyma at the bottom for ease of comparison with panel b. Scale = 50 µm. e-e′′′ As revealed by IHC, cells of the meninges co-express MRC1 (e, yellow), LYVE1 (e′, white), & VEGFR3 (e′′, green). Red arrows highlight cells expressing these three markers. The images are rotated with the parenchyma at the bottom. scale = 30 µm. f, g Quantification of the relative numbers of single & double-labelled cells in 2-month old mouse meninges. VEGFR3 & LYVE1 cell counts were from n = 2 brains, 3 coronal sections (10 area images)/brain. MRC1 & LYVE1 cell counts were from n = 3 brains, 3 coronal sections (4 area images)/brain. The mean values for each set are depicted Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-310202416203723.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), & MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), & confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); & 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), & MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology & confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), & VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) & images are merged in (k, p). Scale = 10 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-310202416205169.jpg)
![Immunocytochemistry/ Immunofluorescence: LYVE-1 Antibody (RM0033-4D17) - Azide and BSA Free [NB110-61026] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. a–d As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). DAPI marks the nuclei in blue (d). Scale = 30 µm. n = 3 brains. e–h E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). DAPI marks the nuclei in blue (h). Scale = 30 µm. n = 3 brains. i–l E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), & MRC1 (white). PROX1 is cytoplasmic (j, red arrows) in the meninges but nuclear in neurons (j, blue arrows). DAPI marks the nuclei in blue (l). Scale = 30 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61026_rat-monoclonal-lyve-1-antibody-rm0033-4d17-310202416205126.jpg)
![Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Western-Blot-NBP1-07118-img0002.jpg "Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of MDCK cells (red). GFP-Tagged human Ti-VAMP (green) and merge (yellow).](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Immunocytochemistry-Immunofluorescence-NBP1-07118-img0001.jpg)
![Immunohistochemistry-Paraffin: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of human spleen.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Immunohistochemistry-Paraffin-NBP1-07118-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NBP1-20255] - Mouse bone marrow derived DCs stained with anti-Rab5a antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Rab5a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20255-img0001.jpg "Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NBP1-20255] - Mouse bone marrow derived DCs stained with anti-Rab5a antibody. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25618_rabbit-rab5a-mab-hl1497-azide-and-bsa-free-2120249415977.jpg "Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25618_rabbit-rab5a-mab-hl1497-azide-and-bsa-free-2120249351322.jpg)
![Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog muscle. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25618_rabbit-rab5a-mab-hl1497-azide-and-bsa-free-2120249393530.jpg)
![Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat lymph node. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25618_rabbit-rab5a-mab-hl1497-azide-and-bsa-free-2120249425480.jpg)
![Western Blot: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25618_rabbit-rab5a-mab-hl1497-azide-and-bsa-free-2120249371544.jpg)
![Western Blot: LAMP-1/CD107a Antibody [NBP1-77241] - Analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-Western-Blot-NBP1-77241-img0011.jpg "Western Blot: LAMP-1/CD107a Antibody [NBP1-77241] - Analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunohistochemistry of LAMP-1/CD107a in rat colon tissue with LAMP-1/CD107a antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77241_rabbit-polyclonal-lamp-1-cd107a-antibody-271120242074610.gif)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunofluorescence of LAMP-1/CD107a in Human Colon cells with LAMP-1/CD107a antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77241_rabbit-polyclonal-lamp-1-cd107a-antibody-271120242013122.jpg)
![Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NBP1-77241] - Immunofluorescence of LAMP-1/CD107a in rat colon tissue with LAMP-1/CD107a antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77241_rabbit-polyclonal-lamp-1-cd107a-antibody-2711202419172227.gif)
![Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDa Observed band size:120 kDa](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Western-Blot-NBP2-89844-img0005.jpg "Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDa Observed band size:120 kDa")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LAMP1 monoclonal antibody (1:300). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor(R) 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunocytochemistry-Immunofluorescence-NBP2-89844-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0002.jpg)
![Flow Cytometry: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated, stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Flow-Cytometry-NBP2-89844-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Staining of human LAMP1 in human placenta with rabbit monoclonal antibody (1:1000). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunohistochemistry-Paraffin-NBP2-89844-img0001.jpg)
![Immunoprecipitation: LAMP-1/CD107a Antibody (107) [NBP2-89844] - Lane A:0.5 mg Jurkat Whole Cell Lysate 1 uL anti-LAMP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LAMP1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size: 113 kDa](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-107-Immunoprecipitation-NBP2-89844-img0006.jpg)
![LAMP-1/CD107a was immunoprecipitated using NBP2-89844 at a 1:100 dilution and 60ug Immunomagnetic beads Protein A/G and Goat Anti-Rabbit IgG (H+L)[HRP] as a secondary antibody at 1/10000 dilution: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg Daudi Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 120 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-4520231646141.png)
![<b>Genetic Strategies Validation. </b>Western Blot: LAMP-1/CD107a Antibody (107) [NBP2-89844] - NBP2-89844 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: LAMP-1/CD107a knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 120 kDa. Observed band size: 100-130 kDa](http://images.novusbio.com/fullsize/nbp2-89844_rabbit-lamp-1-cd107a-mab-107-136202422345046.png)
![Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human skeletal muscle shows only very low cytoplasmic immunoreactivity.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0006.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human kidney shows strong granular cytoplasmic immunoreactivity in renal glomerulus and tubules cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human fallopian tube shows granular cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-NBP2-61619-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL3482) [NBP2-61619] - Staining of human small intestine shows granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL3482-Immunohistochemistry-Paraffin-NBP2-61619-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Syntaxin 7 Antibody (CL0257) [NBP2-52893] - Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate](http://images.novusbio.com/fullsize/Syntaxin-7-Antibody-CL0257-Western-Blot-NBP2-52893-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP2-52893] - Staining of U-251 cells using the Anti-STX7 monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Syntaxin-7-Antibody-CL0257-Immunocytochemistry-Immunofluorescence-NBP2-52893-img0005.jpg)
![Western Blot: RAP1B Antibody [NBP2-20072] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAP1B-Antibody-Western-Blot-NBP2-20072-img0004.jpg "Western Blot: RAP1B Antibody [NBP2-20072] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: RAP1B Antibody [NBP2-20072] - Immunohistochemical analysis of paraffin-embedded lung AdCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RAP1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20072-img0002.jpg)
![Western Blot: RAP1B Antibody [NBP2-20072] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/RAP1B-Antibody-Western-Blot-NBP2-20072-img0001.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - (A) Lysates from bead stimulated human CD8+ T cells transfected with either control or VAMP7 siRNAs (1 or 2, respectively) and blotted for VAMP7 (top) and GAPDH (bottom) as loading control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.01855/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Knockdown-Validated-NBP2-32232-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VAMP-7 Antibody [NBP2-32232] - (A) Lysates from bead stimulated human CD8+ T cells transfected with either control or VAMP7 siRNAs (1 or 2, respectively) and blotted for VAMP7 (top) and GAPDH (bottom) as loading control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.01855/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Western-Blot-NBP2-32232-img0001.jpg)
![Immunoprecipitation: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for IP at 6 ug per reaction. VAMP7 was also immunoprecipitated by rabbit anti-VAMP7 antibody BL16273. For blotting immunoprecipitated VAMP7, NBP2-32232 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunoprecipitation-NBP2-32232-img0002.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - VAMP7 forms a SNARE complex with SNAP-23 & Syntaxin11. (A) Model illustrating the formation of SNARE complex between VAMP7 (v-SNARE), SNAP-23, & Syntaxin11 (t- SNARE) during cytotoxic granule fusion in human CD8+ T cells. (B) Different Twin-Strep-tag fusion constructs used for pulldown assay. Twin-Strep-tag was fused at the C terminus of VAMP7 & at the N terminus of Syntaxin11 with a (GGS)x3 linker. (C) Western blot of bead stimulated human CD8+ T cells transfected with Twin-Strep-tag-tagged VAMP7, immuno-precipitated with anti-FLAG antibody & detected with antibodies against FLAG, SNAP-23, & Syntaxin11. (D) Western blot of bead stimulated human CD8+ T cells transfected with Twin-Strep-tag-tagged Syntaxin11, immuno-precipitated with anti-Syntaxin antibody & detected with antibodies against Strep-tag, SNAP-23, VAMP7, & VAMP2. As control, cells were transfected with Twin-Strep-tag construct. Ten percentage of the lysates were loaded as input. (E,F) Densitometric quantification of the Western blots shown in (C,D), respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416114749.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - Knockdown of VAMP7 strongly reduces fusion of cytotoxic granules at the IS. (A) Lysates from bead stimulated human CD8+ T cells transfected with either control or VAMP7 siRNAs (1 or 2, respectively) & blotted for VAMP7 (top) & GAPDH (bottom) as loading control. (B) Quantification of VAMP7 protein expression (in % normalized to control siRNA-treated CTLs) performed by densitometry. Bars indicate SEMs. [VAMP7-siRNA1, N = 3; ***p < 0.001 & VAMP7-siRNA2, N = 3; ***p < 0.001 (t-test)]. (C) Human CD8+ T cells co-transfected with granzyme B-mCherry along with either ns-siRNA or VAMP7-siRNA1 or VAMP7-siRNA2 & imaged 12 h after transfection. Selected live-cell TIRF microscopy images of granzyme B-mCherry in a transfected CTL in contact with an anti-CD3 coated coverslip. Fusion events are indicated with open circles (three frames shown per granule fused). (D) Mean percentage of cytotoxic granule fusion in cells transfected with either ns-siRNA (n = 66 & n = 59, respectively) or VAMP7-siRNA1 [n = 91; **p < 0.01 (t-test)] or VAMP7-siRNA2 [n = 72; ***p < 0.001 (t-test)]. (E) Mean average number of granules fused over time in the TIRF plane per cell p = 0.206 (t-test) for VAMP7-siRNA1 & **p < 0.01 (t-test) for VAMP7-siRNA2. Bars indicate mean ± SEM. Scale bar, 5 μm. (F) Calcein-based killing assay for CTLs transfected with either ns-siRNA, VAMP7-siRNA1, or VAMP7-siRNA2. Experiments were carried out in duplicate [N = 4; ***p < 0.001 (t-test)]. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416121985.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - Fusion of cytotoxic granules with the plasma membrane is insensitive to Tetanus toxin. (A) Bead-stimulated human CD8+ T cells transfected with GFP or TeNT-GFP as indicated. VAMP2 & VAMP7 protein levels were determined by Western blot analysis 12–16 h after transfection. (B) Expression of VAMP2 & VAMP7 protein levels relative to GAPDH in CTLs transfected with GFP or TeNT-GFP as indicated. Graphs represent means [N = 3, ***p < 0.001 (Student's t-test)]. (C) Bead-stimulated human CTLs co-transfected with either GFP or TeNT-GFP along with granzyme B-mCherry & imaged 12 h after transfection. Representative live-cell TIRFM images of CTLs in contact with an anti-CD3 coated coverslip. Fusion events indicated with open arrowheads. (D) Mean percentage of cytotoxic granule fusion in cells transfected with either GFP (n = 50) or TeNT-GFP (n = 40), p = 0.704 (Student's t-test). (E) Mean average number of granules fused per cell over time in the TIRF plane in cells transfected with either GFP (n = 20) or TeNT-GFP (n = 15), p = 0.939 (Student's t-test). Bars show mean ± SEM. Scale bar, 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416114764.jpg)
![Western Blot: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Western-Blot-NBP2-59053-img0007.jpg "Western Blot: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Analysis in human cell line RT-4.")
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human skeletal muscle shows only weak cytoplasmic positivity in muscle fibers.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human cervix shows granular cytoplasmic immunoreactivity in squamous epithelium cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human kidney shows strong granular cytoplasmic positivity in renal tubules and glomeruli.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human rectum shows granular cytoplasmic immunoreactivity in glandular cells and in lymphoid cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human endometrium shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (CL4489) [NBP2-59053] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous tubules.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-CL4489-Immunohistochemistry-Paraffin-NBP2-59053-img0005.jpg)