| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44433 | |||
| BSA Free | NBP2-42379-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-448SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84797-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06277SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44467 | |||
| BSA Free | NBP2-36765-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85519-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48051 | |||
| Azide and BSA Free | NBP3-11500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24698SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89969-25ul | |||
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U251 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0010.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U2OS cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0017.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0018.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0020.jpg)
![Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Western-Blot-NB100-448-img0005.jpg "Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.")
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-448-img0001.jpg)
![Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-NB100-448-img0006.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes and chaperones in proband and control cells. Proband fibroblasts show increases in PDI, LH1, LH2 and BiP protein levels. CyPB and FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts and osteoblasts. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0009.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes and chaperones in proband and control cells. Proband fibroblasts show increases in PDI, LH1, LH2 and BiP protein levels. CyPB and FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts and osteoblasts. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of purified antibody to P4HB on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-H00005034-B01P-img0005.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in HeLa.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0006.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in transfected 293T cell line by P4HB polyclonal antibody. Lane 1: P4HB transfected lysate(55.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0008.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Analysis of P4HB expression in human liver.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-H00005034-B01P-img0007.jpg)
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [H00005034-B01P] - Absence of TRIC-B alters expression of collagen-interacting proteins.(A) Quantitative RT-PCR of fibroblast transcripts encoding collagen, modifying enzymes & chaperones. Proband cells (P1, P2, & P3) demonstrate decreased expression of COL1A1 & FKBP10 (FKBP65), & increased expression of PLOD1 (LH1) & HSPA5 (BiP/GRP78) versus control fibroblasts (C). (B) Decreased expression of PLOD2 (LH2), FKBP10 (FKBP65) & PPIB (CyPB) in P2 osteoblasts versus control osteoblasts (C). (C) Immunoblots for quantitation of steady-state protein levels of collagen modifying enzymes & chaperones in proband & control cells. Proband fibroblasts show increases in PDI, LH1, LH2 & BiP protein levels. CyPB & FKBP65, both collagen-interacting isomerases, are consistently decreased in proband fibroblasts & osteoblasts. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006156), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005034-b01p_mouse-polyclonal-protein-disulfide-isomerase-p4hb-antibody-31020241548512.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human gastrointestinal, prostate, skeletal muscle and thyroid gland using Anti-PDIA3 antibody NBP1-84797 (A) shows similar protein distribution across tissues to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis using Anti-PDIA3 antibody NBP1-84797 (A) shows similar pattern to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NBP1-84797-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP1-84797] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84797-img0007.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0018.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0019.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0020.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0021.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06277-img0005.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06277] - BIP/Grp78 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06277-img0006.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0008.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band detected for GRP78 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0007.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in human thyroid gland and skeletal muscle tissues. Corresponding PDIA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunohistochemistry-Paraffin-NBP2-36765-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in human thyroid gland and skeletal muscle tissues. Corresponding PDIA3 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Western-Blot-NBP2-36765-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in U251 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunocytochemistry-Immunofluorescence-NBP2-36765-img0011.jpg)
![Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Western-Blot-NBP2-36765-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunocytochemistry-Immunofluorescence-NBP2-36765-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunocytochemistry-Immunofluorescence-NBP2-36765-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunocytochemistry-Immunofluorescence-NBP2-36765-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in U2OS cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunocytochemistry-Immunofluorescence-NBP2-36765-img0010.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human thyroid gland shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunohistochemistry-Paraffin-NBP2-36765-img0013.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunohistochemistry-Paraffin-NBP2-36765-img0014.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunohistochemistry-Paraffin-NBP2-36765-img0015.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-CL2444-Immunohistochemistry-Paraffin-NBP2-36765-img0016.jpg)
![Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-85519-img0018.jpg "Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-85519] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85519-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0017.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85519 (A) shows similar protein distribution across tissues to independent antibody NBP1-85520 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0015.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0016.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0020.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16749-img0018.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat duodenum. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0016.jpg)
![Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GRP78-Antibody-Western-Blot-NBP2-16749-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 7.5% SDS-PAGE Grp78 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16749-img0017.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0006.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse testis. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat liver. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0015.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - GRP78/HSPA5 proteins were measured by immunoblotting in epithelioid (EPI BAP1+ UPN7), biphasic (BIP BAP1- UPN16) and sarcomatous (BAP1+ UPN22) MPM cells. Tubulin was used as a loading control. The figure is representative of 1 out of 3 experiments with similar results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35197103/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-25520231539516.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-1610202419405722.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-32118-img0011.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32118-img0002.jpg)
![Immunohistochemistry: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse lung. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-NBP1-32118-img0007.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Sample(30 ug of whole cell lysate)A:293TB:A431 7. 5% SDS PAGE, antibody diluted at 1:1500.](http://images.novusbio.com/fullsize/GRP94-Antibody-Western-Blot-NBP1-32118-img0005.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody diluted at 1:1500. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-32118-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - GRP94 Antibody [NBP1-32118] - Paraffin-embedded BT483 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-32118-img0003.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Mouse skin. HSP90 beta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-32118-img0006.jpg)
![IHC-WM-gp96/HSP90B1/GRP94 Antibody [NBP1-32118] -Hsp90 beta antibody detects Hsp90 beta protein on whole-mount zebrafish embryos by immunohistochemical analysis.Sample: Paraformaldehyde-fixed zebrafish embryos.Hsp90 beta antibody dilution: 1:200.](http://images.novusbio.com/fullsize/antibody/nbp1-32118_rabbit-polyclonal-gp96-hsp90b1-grp94-antibody-immunohistochemistry-whole-mount-3011202314722..jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-32118] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE NBP1-32118 diluted at 1:10000](http://images.novusbio.com/fullsize/nbp1-32118_rabbit-polyclonal-gp96-hsp90b1-grp94-antibody-910202420131243.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Immunohistochemistry-Paraffin-NBP2-44690-img0001.jpg "Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).")
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using gp96/HSP90B1/GRP94 Antibody (SPM249).followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-SPM249-Flow-Cytometry-NBP2-44690-img0002.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (SPM249) [NBP2-44690] - Classical swine fever virus (CSFV) infection induces the activation of UPR. (A) RNA extracted from CSFV-infected cells was quantified for the expression of UPR genes Xbp1(s), GRP78, GRP94, EDEM-1, ATF4, ATF6, CHOP, Calreticulin, Calnexin, & ERp57 using q-PCR. Mock-infected PK-15 & Thapsigargin (TG)-treated PK-15 were used as negative & positive controls, respectively, & the fold induction was calculated compared to mock cells at the same time point. Error bars represent the mean ± SD of 3 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. (B,D,E) Immunoblotting analysis of components of UPR signaling pathways during a time course of CSFV infection. Mock or CSFV-infected PK-15 cells lysates were collected at the indicated time points. Lysates were analyzed for the activation of the IRE1 (B), PERK (D) & ATF6 (E) pathway by immunoblotting analysis. Tubulin was used as a loading control, & infection was confirmed by detecting the viral protein Npro. Results of a representative experiment of 2 independent experiments are shown. (C) RNA was collected as described above, & the splicing levels of XBP1 were analyzed with semi-quantitative PCR as described in materials & methods. The length of Xbp1(u) is 474 bp & Xbp1(s) is 448 bp. (F) The relative expression ratios of the targeted proteins/genes were analyzed by densitometric scanning. Error bars represent the mean ± SD of 2 independent experiments; one-way ANOVA test; ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fmicb.2017.02129/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44690_rat-monoclonal-gp96-hsp90b1-grp94-antibody-spm249-31020241612223.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of human GRP94 in 3T3 cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24698 at 1 ug/ml.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP2-24698-img0006.jpg "Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of human GRP94 in 3T3 cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24698 at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - GPR94 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24698-img0004.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP2-24698] - Analysis of Human Placenta using NBP2-24698 at 10 ug/ml.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP2-24698-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0009.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0011.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human rectum shows strong cytoplasmic positivity in glandular and lymphoid cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP1-89969] - Analysis using Anti-HSPA5 antibody NBP1-89969 (A) shows similar pattern to independent antibody NBP1-89968 (B).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89969-img0014.jpg)