| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44020 | |||
| BSA Free | NBP2-34491-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61764-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85568-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47797-0.1mg | |||
| IHC-Prediluted | NBP2-48309 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38895-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43926 | |||
| BSA Free | NBP2-34478-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70687 | |||
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84827 (A) shows similar protein distribution across tissues to independent antibody NBP1-84826 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cell line MCF7 shows localization to nucleus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84827-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0024.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0022.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0023.jpg)
![Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Western-Blot-NBP2-67605-img0006.jpg "Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0002.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0001.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0003.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0004.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0005.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0007.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining in human tonsil and kidney tissues. Corresponding ITGAX RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD11c-Antibody-CL1831-Immunohistochemistry-Paraffin-NBP2-34491-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining in human tonsil and kidney tissues. Corresponding ITGAX RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD11c Antibody (CL1831) [NBP2-34491] - Lane 1: Marker [kDa]. Lane 2: Human tonsil.](http://images.novusbio.com/fullsize/CD11c-Antibody-CL1831-Western-Blot-NBP2-34491-img0001.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - CD11c Positive cells in mouse liver tissue. Primary antibody dilution: 1:100. Citrate buffer antigen retrieval. Overnight incubation RT. Image from verified customer review.](http://images.novusbio.com/fullsize/CD11c-Antibody-CL1831-Immunohistochemistry-Paraffin-NBP2-34491-img0017.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human tonsil shows strong membranous positivity in a subset of germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/CD11c-Antibody-CL1831-Immunohistochemistry-Paraffin-NBP2-34491-img0012.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human small intestine shows moderate positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD11c-Antibody-CL1831-Immunohistochemistry-Paraffin-NBP2-34491-img0013.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human fallopian tube shows moderate membranous positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/CD11c-Antibody-CL1831-Immunohistochemistry-Paraffin-NBP2-34491-img0014.jpg)
![Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/CD11c-Antibody-CL1831-Immunohistochemistry-Paraffin-NBP2-34491-img0015.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0008.jpg "Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0006.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0007.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0003.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0005.jpg)
![ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0001.jpg)
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0010.jpg "Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) Pacific BlueTM.")
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP (monocyte gate).](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0001.jpg)
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) APC.](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0002.jpg)
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0003.jpg)
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PE.](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0004.jpg)
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PE-CyTM7. .](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0005.jpg)
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP.](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0007.jpg)
![Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) FITC.](http://images.novusbio.com/fullsize/CD11c-Antibody-BU15-Flow-Cytometry-NBP1-45018-img0008.jpg)
![Western Blot: CLIP170 Antibody [NBP1-85568] - Analysis in human cell line HDLM-2 and human cell line U-2197.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Western-Blot-NBP1-85568-img0012.jpg "Western Blot: CLIP170 Antibody [NBP1-85568] - Analysis in human cell line HDLM-2 and human cell line U-2197.")
![Immunocytochemistry/Immunofluorescence: CLIP170 Antibody [NBP1-85568] - Staining of human cell line SiHa shows localization to microtubule ends. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85568-img0011.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-85568] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myoctes.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Immunohistochemistry-Paraffin-NBP1-85568-img0015.jpg)
![Western Blot: CLIP170 Antibody [NBP1-85568] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Western-Blot-NBP1-85568-img0008.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-85568] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Immunohistochemistry-Paraffin-NBP1-85568-img0002.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-85568] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Immunohistochemistry-Paraffin-NBP1-85568-img0013.jpg)
![Immunohistochemistry-Paraffin: CLIP170 Antibody [NBP1-85568] - Staining of human liver shows negative positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CLIP170-Antibody-Immunohistochemistry-Paraffin-NBP1-85568-img0014.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0006.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).")
![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Flow-Cytometry-NBP2-44366-img0010.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0003.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0004.jpg)
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Immunohistochemistry-Paraffin-NBP2-44366-img0005.jpg)
![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Flow-Cytometry-NBP2-44366-img0009.jpg)
![Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-western-blot-1311202411142..png "Western Blot: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Whole cell lysate (10 µg) from HEK293T, HDLM-2, Jurkat,MJ, and HeLa cells prepared using NETN lysis buffer.Antibody: Rabbit anti-CD86 recombinant monoclonalantibody [BLR030F] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with anexposure time of 10 seconds.")
![Flow Cytometry: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Antibody: Rabbit anti-CD86 recombinantmonoclonal [BLR030F] or isotype control(unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-flow-cytometry-1311202411333..png)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -FFPE section of tonsil. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunohistochemistry-paraffin-131120241151..png)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] -Section of HDLM-2 cells. Antibody: Rabbit anti-CD86recombinant monoclonal antibody [BLR030F]. Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunocytochemistry-immunofluorescence-1311202411712..png)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: NBP2-76417 Lot 4 used for immunoprecipitation at 6 ul per reaction. B7-2/CD86 was also immunoprecipitated by a previous lot of this antibody (NBP2-76417 Lot 1) and a second antibody, NBP2-76416, against a different epitope of B7-2/CD86 (BLR029F). For blotting immunoprecipitated CD86, NBP2-76417 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-1312025145029..jpg)
![Immunoprecipitation: B7-2/CD86 Antibody (BLR030F) [NBP2-76417] - Detection of human CD86 by WB of immunoprecipitates from HDML-2 lysate. Antibodies: Rabbit anti-CD86 recombinant monoclonal [BLR030F] (NBP2-76417) and rabbit anti-CD86 recombinant monoclonal [BLR029F] (NBP2-76416).](http://images.novusbio.com/fullsize/nbp2-76417_rabbit-monoclonal-b7-2-cd86-antibody-blr030f-immunoprecipitation-131202515750..jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] -Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38895-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human endometrium, kidney, lymph node and tonsil using Anti-CD21 antibody NBP2-38895 (A) shows similar protein distribution across tissues to independent antibody NBP2-38684 (B).](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0010.jpg)
![Western Blot: CD21 Antibody [NBP2-38895] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/CD21-Antibody-Western-Blot-NBP2-38895-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human lymph node shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0007.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human tonsil shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0008.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human kidney shows very weak membranous positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/CD21-Antibody-Immunohistochemistry-Paraffin-NBP2-38895-img0009.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Western-Blot-NBP2-34478-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of MCF7 cells using the Anti-ESR1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunocytochemistry-Immunofluorescence-NBP2-34478-img0009.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human testis shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human endometrium shows strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0001.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human cervix shows strong nuclear positivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human fallopian tube shows strong immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (CL1196) [NBP2-34478] - Staining of human breast cancer shows moderate to strong nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-CL1196-Immunohistochemistry-NBP2-34478-img0004.jpg)
![Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Western-Blot-NBP2-01371-img0001.jpg "Western Blot: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Estrogen Receptor alpha.")
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0009.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0002.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0003.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0004.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0005.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0006.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0007.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor alpha Antibody (1B1) [NBP2-01371] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Estrogen-Receptor-alpha-Antibody-1B1-Immunohistochemistry-Paraffin-NBP2-01371-img0008.jpg)
