| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62735SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26427-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26258-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59614-100ug | |||
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-71931-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HSF1 Antibody [NBP1-71931] - Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HSF1 Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (center) and Cat. No. Lot1 used at a dilution](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-NBP1-71931-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody NBP1-71931 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by two other rabbit anti-HSF1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunoprecipitation-NBP1-71931-img0003.jpg)
![Western Blot: STAG2 Antibody [NBP1-30472] - Western Blot and Immunoprecipitation: Whole cell lysate from HeLa and 293T cells. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody NBP1-30473.](http://images.novusbio.com/fullsize/STAG2-Antibody-Western-Blot-NBP1-30472-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: STAG2 Antibody [NBP1-30472] - Western Blot and Immunoprecipitation: Whole cell lysate from HeLa and 293T cells. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody NBP1-30473.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: STAG2 Antibody [NBP1-30472] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SA2 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/STAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-30472-img0002.jpg)
![Western Blot: KIF14 Antibody [NB100-254] - Detection of Human KIF14 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIF14 antibody NB100-254 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KIF14-Antibody-Western-Blot-NB100-254-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: KIF14 Antibody [NB100-254] - Detection of Human KIF14 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIF14 antibody NB100-254 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: KIF14 Antibody [NB100-254] - Detection of human KIF14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIF14 antibody NB100-254 (lot 2) used for IP at 6 ug per reaction. KIF14 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-KIF14 antibody NB100-243. For blotting immunoprecipitated KIF14, NB100-254 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KIF14-Antibody-Immunoprecipitation-NB100-254-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.](http://images.novusbio.com/fullsize/Chk2-Antibody-8F12-Immunocytochemistry-Immunofluorescence-NB100-500-img0003.jpg "Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.")
![Western Blot: Chk2 Antibody (8F12) [NB100-500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (NB100-500) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-500_mouse-monoclonal-chk2-antibody-8f12-91020242014681.jpg)
![Western Blot: CRISPR-Cpf1 Antibody [NBP2-62735] - Partial recombinant Cpf1 protein (Ca. 12 kDa) was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Cpf1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Lane 1, 10 ng, Lane 2. 5 ng.](http://images.novusbio.com/fullsize/CRISPR-Cpf1-Antibody-Western-Blot-NBP2-62735-img0001.jpg "Western Blot: CRISPR-Cpf1 Antibody [NBP2-62735] - Partial recombinant Cpf1 protein (Ca. 12 kDa) was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Cpf1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Lane 1, 10 ng, Lane 2. 5 ng.")
![Western Blot: DNA Ligase I Antibody [NB100-56635] - Analysis of LIG1 in cell lysate from HeLa cells using this antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-Western-Blot-NB100-56635-img0002.jpg "Western Blot: DNA Ligase I Antibody [NB100-56635] - Analysis of LIG1 in cell lysate from HeLa cells using this antibody.")
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Western-Blot-NBP2-42860-img0002.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000")
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/Histone-H4-Dimethyl-Lys20-Antibody-282-Chromatin-Immunoprecipitation-ChIP-NBP2-42860-img0001.jpg)
![Histone H4 (di-Methyl Lys20) antibody [GT282] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT282] (NBP2-42860) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42860_mouse-monoclonal-histone-h4-dimethyl-lys20-antibody-282-immunocytochemistry-immunofluorescence-26112025198364.jpg)
![Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/XPA-Antibody-Western-Blot-NB100-93321-img0009.jpg "Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.](http://images.novusbio.com/fullsize/XPA-Antibody-Immunohistochemistry-NB100-93321-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: XPA Antibody [NB100-93321] - Detection of human XPA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XPA antibody NB100-93321 (lot NB100-93321-3) used for IP at 6 ug per reaction. XPA was also immunoprecipitated by a previous lot of this antibody (lot NB100-93321-2) and another rabbit anti-XPA antibody. For blotting immunoprecipitated XPA, NB100-93321 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/XPA-Antibody-Immunoprecipitation-NB100-93321-img0007.jpg)
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202414471571.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202415475635.jpg)
![Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: eIF2 alpha/EIF2S1 [p Ser51] Antibody at 1.48ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 KDa Observed band size: 36 KDa](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202411442641.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Staining of A549 cells (treated with 100ng/ml EGF for 20min) with eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202412493624.jpg)
![Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-262202415253324.jpg "Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: USP7 Antibody (7F10) [NBP3-26258] - Image of USP7 Antibody (7F10) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-2722024827493.jpg)
![Western Blot: USP7 Antibody (7F10) [NBP3-26258] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: USP7 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 129, 127 kDa Observed band size: 140 kDa](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-282202411363024.jpg)
![Immunocytochemistry/Immunofluorescence: USP7 Antibody (7F10) [NBP3-26258] - Staining of Hela Cells with USP7 Antibody (7F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26258_rabbit-usp7-mab-7f10-28220241312224.jpg)
![Western Blot: HP1 alpha Antibody [NBP2-24602] - Analysis of CBX5 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HP1-alpha-Antibody-Western-Blot-NBP2-24602-img0001.jpg "Western Blot: HP1 alpha Antibody [NBP2-24602] - Analysis of CBX5 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Western-Blot-NBP2-59613-img0004.jpg "Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Immunohistochemistry-Paraffin-NBP2-59613-img0001.jpg)
![Protein Array: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Protein-Array-NBP2-59613-img0005.jpg)