| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-18978SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59609-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
![Western Blot: MSH6 Antibody [NB100-329] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH6 antibody NB100-329 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MSH6-Antibody-Western-Blot-NB100-329-img0029.jpg "<b>Biological Strategies Validation. </b>Western Blot: MSH6 Antibody [NB100-329] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH6 antibody NB100-329 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-329] - Section of human testicular seminoma.Antibody: Affinity purified rabbit anti- MSH6used at a dilution of 1:500 (2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MSH6-Antibody-Immunohistochemistry-NB100-329-img0027.jpg)
![Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-281-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-281] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NB600-281-img0013.jpg)
![Chromatin Immunoprecipitation: ASH2L Antibody [NB600-281] - ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-281 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products abeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Chromatin-Immunoprecipitation-NB600-281-img0009.jpg)
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0022.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - Detection of Human CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-376 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-376] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-376-img0016.jpg)
![Western Blot: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody NB100-299 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SA1-Antibody-Western-Blot-NB100-299-img0009.jpg "Western Blot: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody NB100-299 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-299] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Immunocytochemistry-NB100-299-img0005.jpg)
![Western Blot: SA1 Antibody [NB100-299] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Western-Blot-NB100-299-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SA1 Antibody [NB100-299] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SA1-Antibody-Western-Blot-NB100-299-img0007.jpg)
![Immunoprecipitation: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody NB100-299 (lot NB100-299-3) used for IP at 6 ug per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-299-2). For blotting immunoprecipitated SA1, NB100-299 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SA1-Antibody-Immunoprecipitation-NB100-299-img0008.jpg)
![Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Western-Blot-NBP3-15177-img0005.jpg "Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: hnRNP K Antibody (3G7) [NBP3-15177] - Immunofluorescence staining of A549 cells with at hnRNP K antibody 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunocytochemistry-Immunofluorescence-NBP3-15177-img0001.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0004.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0003.jpg)
![Immunoprecipitation: hnRNP K Antibody (3G7) [NBP3-15177] - Immunoprecipitating hnRNP K in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of hnRNP K antibody in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: hnRNP K antibody (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunoprecipitation-NBP3-15177-img0002.jpg)
![Western Blot: PRP19 Antibody [NBP1-33117] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP1-33117-img0005.jpg "Western Blot: PRP19 Antibody [NBP1-33117] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP1-33117] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33117-img0003.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP1-33117] - Paraffin-embedded HT29 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP1-33117-img0004.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP1-33117] - PRP19 antibody detects PRP19 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PRP19 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP1-33117-img0006.jpg)
![Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20--p-Ser24]-Antibody-Western-Blot-NBP1-78102-img0001.jpg "Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.")
![Immunohistochemistry: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20-p-Ser24]-Antibody-Immunohistochemistry-NBP1-78102-img0003.jpg)
![Western Blot: FANCC Antibody [NBP1-18978] - Western Blot on cells overexpressing FANCC probed with 1. pre-immune serum and 2.](http://images.novusbio.com/fullsize/FANCC-Antibody-Western-Blot-NBP1-18978-img0002.jpg "Western Blot: FANCC Antibody [NBP1-18978] - Western Blot on cells overexpressing FANCC probed with 1. pre-immune serum and 2.")
![Western Blot: MCM7 Antibody (MCM7/1467) [NBP2-59608] - Western Blot Analysis of MCF-7, HEK-293, A549 lysate using MCM7 Antibody (MCM7/1467).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1467-Western-Blot-NBP2-59608-img0004.jpg "Western Blot: MCM7 Antibody (MCM7/1467) [NBP2-59608] - Western Blot Analysis of MCF-7, HEK-293, A549 lysate using MCM7 Antibody (MCM7/1467).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1467) [NBP2-59608] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1467).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1467-Immunohistochemistry-Paraffin-NBP2-59608-img0001.jpg)
![Protein Array: MCM7 Antibody (MCM7/1467) [NBP2-59608] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Antibody (MCM7/1467) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1467-Protein-Array-NBP2-59608-img0003.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)