| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97936-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12658-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06445-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99020-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09017-0.025mg | |||
![Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-278202415242677.jpg "Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32444) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-29820241420917.jpg)
![Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunocytochemistry analysis of HeLa cells labeling HSF1 with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-2920241033401.jpg)
![Flow Cytometry: HSF1 Antibody (JE44-79) [NBP3-32444] - Flow cytometric analysis of HeLa cells labeling HSF1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32444, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-392024884655.jpg)
![Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP2-97936-img0005.jpg "Western Blot: RFC4 Antibody [NBP2-97936] - Anti-RFC4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-97936] - Immunochemical staining of human RFC4 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-97936-img0004.jpg)
![Immunoprecipitation: RFC4 Antibody [NBP2-97936] - RFC4 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RFC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RFC4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunoprecipitation-NBP2-97936-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-97936] - Immunofluorescence staining of RFC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97936 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97936_rabbit-polyclonal-rfc4-antibody-23520251664787.png)
![Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-278202416331255.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29820241426265.jpg)
![Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunocytochemistry analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A with Rabbit anti-p21/CIP1/CDKN1A/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202410334083.jpg)
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Flow cytometric analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202416372178.jpg)
![Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-27820241631683.jpg "Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32576) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-298202414262619.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunocytochemistry analysis of LOVO cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241114811.jpg)
![Flow Cytometry: MCM2 Antibody (PD00-90) [NBP3-32576] - Flow cytometric analysis of HL-60 cells labeling MCM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32576, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241637215.jpg)
![Western Blot: CC2D1A Antibody [NBP3-12658] - Lane A: Hela Whole Cell LysateLane B: HEK-293 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:130 kDa](http://images.novusbio.com/fullsize/CC2D1A-Antibody-Western-Blot-NBP3-12658-img0001.jpg "Western Blot: CC2D1A Antibody [NBP3-12658] - Lane A: Hela Whole Cell LysateLane B: HEK-293 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:130 kDa")
![Immunohistochemistry-Paraffin: CC2D1A Antibody [NBP3-12658] - Staining of human CC2D1A in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CC2D1A-Antibody-Immunohistochemistry-Paraffin-NBP3-12658-img0002.jpg)
![Immunoprecipitation: CC2D1A Antibody [NBP3-12658] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-CC2D1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CC2D1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 104 kDaObserved band size :130 kDa](http://images.novusbio.com/fullsize/CC2D1A-Antibody-Immunoprecipitation-NBP3-12658-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CC2D1A Antibody - BSA Free [NBP3-12658] - Immunofluorescence staining of CC2D1A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12658 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12658_rabbit-polyclonal-cc2d1a-antibody-235202514331558.png)
![Western Blot: NASP Antibody [NBP3-06445] - NASP was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate. 4 uL anti-NASP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NASP rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :180 kDa.](http://images.novusbio.com/fullsize/NASP-Antibody-Western-Blot-NBP3-06445-img0004.jpg "Western Blot: NASP Antibody [NBP3-06445] - NASP was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate. 4 uL anti-NASP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NASP rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :180 kDa.")
![Immunohistochemistry-Paraffin: NASP Antibody [NBP3-06445] - Immunochemical staining of human NASP in human rectum with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NASP-Antibody-Immunohistochemistry-Paraffin-NBP3-06445-img0002.jpg)
![Western Blot: NASP Antibody [NBP3-06445] - Anti-NASP rabbit polyclonal antibody at 1:2000 dilution. Lane A: 293T Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:180 kDa.](http://images.novusbio.com/fullsize/NASP-Antibody-Western-Blot-NBP3-06445-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NASP Antibody [NBP3-06445] - Immunofluorescence staining of NASP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06445 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06445_rabbit-polyclonal-nasp-antibody-23520251664715.png)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRPF19 in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0005.jpg "Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRPF19 in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRP19 in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0002.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRP19 in human colon carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0003.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRP19 in mouse colon with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP2-99020] - Immunofluorescence staining of PRP19 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99020 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-99020_rabbit-polyclonal-prp19-antibody-2352025154430.png)
![Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Western-Blot-NBP3-09017-img0003.jpg "Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: MHC Class I Antibody (R1-21.2) - Chimeric - Azide and BSA Free [NBP3-09017] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized and stained with NBP3-09017 at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09017, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-09017_rabbit-monoclonal-mhc-class-i-antibody-r1-21-2-chimeric-74202522265217.png)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Western Blot: MSH6 Antibody [NB100-328] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MSH6 antibody NB100-328 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MSH6-Antibody-Western-Blot-NB100-328-img0058.jpg "<b>Biological Strategies Validation. </b>Western Blot: MSH6 Antibody [NB100-328] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MSH6 antibody NB100-328 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-328] - Section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-MSH6 used at a dilution of 1:500 (2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MSH6-Antibody-Immunohistochemistry-NB100-328-img0056.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Western-Blot-NB100-200-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Nuclear extract (50 ug) from HeLa cells mock irradiated or irradiated with 10 Gy of ionizing radiation. Antibody used at 0.5 ug/ml.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Western-Blot-NB100-200-img0002.jpg)
![Proximity Ligation Assay: BRCA1 [p Ser1524] Antibody [NB100-200] - Image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Proximity-Ligation-Assay-NB100-200-img0005.jpg)