| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05515-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47441-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38864-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80746-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86561-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12890-0.025mg | |||
![Western Blot: ING2 Antibody [NBP3-05515] - Analysis in control (vector only transfected HEK293T lysate) and ING2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.](http://images.novusbio.com/fullsize/ING2-Antibody-Western-Blot-NBP3-05515-img0003.jpg "Western Blot: ING2 Antibody [NBP3-05515] - Analysis in control (vector only transfected HEK293T lysate) and ING2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.")
![Immunocytochemistry/Immunofluorescence: ING2 Antibody [NBP3-05515] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.](http://images.novusbio.com/fullsize/ING2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05515-img0001.jpg)
![Immunohistochemistry-Paraffin: ING2 Antibody [NBP3-05515] - Staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ING2-Antibody-Immunohistochemistry-Paraffin-NBP3-05515-img0002.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human colon.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0009.jpg "Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human colon.")
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0003.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining in human testis and liver tissues using anti-AKAP8L antibody. Corresponding AKAP8L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human cerebral cortex, colon, kidney and testis using Anti-AKAP8L antibody NBP2-47441 (A) shows similar protein distribution across tissues to independent antibody NBP2-47440 (B).](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0006.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human kidney.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0007.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47441] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47441-img0008.jpg)
![Western Blot: PRP19 Antibody [NBP2-38864] - Analysis using Anti-PRPF19 antibody NBP2-38864 (A) shows similar pattern to independent antibody NBP2-38381 (B).](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-38864-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PRP19 Antibody [NBP2-38864] - Analysis using Anti-PRPF19 antibody NBP2-38864 (A) shows similar pattern to independent antibody NBP2-38381 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP2-38864] - Staining of human cell line MCF7 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38864-img0005.jpg)
![Immunohistochemistry: PRP19 Antibody [NBP2-38864] - Staining of human kidney shows strong nuclear positivity in cells in renal tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-NBP2-38864-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PRP19 Antibody [NBP2-38864] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-38864-img0007.jpg)
![Western Blot: SIRT4 Antibody [NBP1-80746] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SIRT4-Antibody-Western-Blot-NBP1-80746-img0004.jpg "Western Blot: SIRT4 Antibody [NBP1-80746] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining in human testis and pancreas tissues using anti-SIRT4 antibody. Corresponding SIRT4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NBP1-80746-img0007.jpg)
![Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NBP1-80746-img0005.jpg)
![Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/SIRT4-Antibody-Immunohistochemistry-Paraffin-NBP1-80746-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86561-img0006.jpg "Immunocytochemistry/Immunofluorescence: Rad17 Antibody [NBP1-86561] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Rad17 Antibody [NBP1-86561] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Rad17-Antibody-Immunohistochemistry-Paraffin-NBP1-86561-img0001.jpg)
![Western Blot: KAP1 Antibody (20C1) [NB100-74549] - Analysis of TIF1 beta in gamma irradiated HeLA cells.](http://images.novusbio.com/fullsize/KAP1-Antibody-20C1-Western-Blot-NB100-74549-img0005.jpg "Western Blot: KAP1 Antibody (20C1) [NB100-74549] - Analysis of TIF1 beta in gamma irradiated HeLA cells.")
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody (20C1) [NB100-74549] - Gamma irradiated HeLa cells.](http://images.novusbio.com/fullsize/KAP1-Antibody-20C1-Immunocytochemistry-Immunofluorescence-NB100-74549-img0004.jpg)
![Western Blot: Cyclin H Antibody (3N5S3) [NBP3-16325] - Western blot analysis of extracts of various cell lines, using Cyclin H Rabbit mAb (NBP3-16325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Cyclin-H-Antibody-3N5S3-Western-Blot-NBP3-16325-img0004.jpg "Western Blot: Cyclin H Antibody (3N5S3) [NBP3-16325] - Western blot analysis of extracts of various cell lines, using Cyclin H Rabbit mAb (NBP3-16325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit mAb (NBP3-16325) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-H-Antibody-3N5S3-Immunocytochemistry-Immunofluorescence-NBP3-16325-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunohistochemistry of paraffin-embedded mouse testis using Cyclin H Rabbit mAb (NBP3-16325) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclin-H-Antibody-3N5S3-Immunohistochemistry-Paraffin-NBP3-16325-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin H Rabbit mAb (NBP3-16325) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-H-Antibody-3N5S3-Immunocytochemistry-Immunofluorescence-NBP3-16325-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16325_rabbit-cyclin-h-mab-3n5s3-5220251255520.jpg)
![Western Blot: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Western blot analysis of extracts of various cell lines, using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-7N7G3-Western-Blot-NBP3-15448-img0003.jpg "Western Blot: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Western blot analysis of extracts of various cell lines, using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Immunohistochemistry of paraffin-embedded human esophageal using Ku80/XRCC5 Rabbit mAb (NBP3-15448) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-7N7G3-Immunohistochemistry-Paraffin-NBP3-15448-img0001.jpg)
![Immunoprecipitation: Ku80/XRCC5 Antibody (7N7G3) [NBP3-15448] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Ku80/XRCC5 antibody (NBP3-15448). Western blot was performed from the immunoprecipitate using Ku80/XRCC5 antibody (NBP3-15448) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-7N7G3-Immunoprecipitation-NBP3-15448-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (7N7G3) [Ku80/XRCC5] - Confocal imaging of HeLa cells using Ku80/XRCC5 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 60x.](http://images.novusbio.com/fullsize/nbp3-15448_rabbit-ku80-xrcc5-mab-7n7g3-52202517152746.jpg)
![Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Western-Blot-NBP2-12890-img0004.jpg "Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890] - Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Immunocytochemistry-Immunofluorescence-NBP2-12890-img0003.jpg)
![Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Western-Blot-NBP2-12890-img0001.jpg)
![Western Blot: eIF4E Antibody (JE60-70) [NBP3-32302] - Western blot analysis of eIF4E on different lysates with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32302) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-278202415212480.jpg "Western Blot: eIF4E Antibody (JE60-70) [NBP3-32302] - Western blot analysis of eIF4E on different lysates with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32302) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4E Antibody (JE60-70) [NBP3-32302] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32302) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-298202414161811.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (JE60-70) [NBP3-32302] - Immunocytochemistry analysis of SK-Br-3 cells labeling eIF4E with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-2920241192325.jpg)
![Flow Cytometry: eIF4E Antibody (JE60-70) [NBP3-32302] - Flow cytometric analysis of SK-Br-3 cells labeling eIF4E. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32302, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-29202416453285.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NBP2-59260-img0001.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Mouse NIH3T3 cells were stained with the antibody against H4K8ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H4K8ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunofluorescence-NBP2-59260-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-59260] - ChIP assays were performed using human HeLa cells, the antibody directed against H4K8ac and optimized PCR primer sets for qPCR. ChIP was performed with a LowCell l# ChIP kit on sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the c-fos promoter and for the Sat2 satellite repeat region. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-59260-img0003.jpg)
![ELISA: Histone H4 [ac Lys8] Antibody [NBP2-59260] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K8ac, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:16,700.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-ELISA-NBP2-59260-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H4K8ac with peptides containing other histone modifications and the unmodified H4K8. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NBP2-59260-img0006.jpg)
![Western Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Western-Blot-NBP2-59252-img0001.jpg "Western Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - HeLa cells were stained with Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody and DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. (Top): cells were immunofluorescently labeled with the Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Subsequent images show staining of the cells with the Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody after incubation of the antibody with 10 ng/ul of the following blocking peptides: H4K5,8,12 unmodified, H4K5,8,12ac, H2A.ZK5,7,11ac and H4K5,8,12,16ac, respectively.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59252-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - ChIP assays were performed using human K562 cells, the antibody against H4K5,8,12ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP- seq kit on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5-ac-Lys8-ac-Lys12]-Antibody-Chromatin-Immunoprecipitation-NBP2-59252-img0003.jpg)
![ELISA: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K5,8,12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-ELISA-NBP2-59252-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody [NBP2-59252] - To test the cross reactivity of Histone H4 [ac Lys5, ac Lys8, ac Lys12] Antibody, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5--ac-Lys8--ac-Lys12]-Antibody-Dot-Blot-NBP2-59252-img0004.jpg)