| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56364SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52726SS | |||
| Azide and BSA Free | NBP2-80943 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56359SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61930SS | |||
| Azide and BSA Free | NBP2-80730 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-2564SS | |||
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33083-img0004.jpg "Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - RFC4 antibody detects RFC4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded MDA-MB-231xenograft. RFC4 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0008.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NBP1-33083-img0006.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - Paraffin-embedded MDAMB-468 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP1-33083-img0005.jpg)
![Western Blot: RFC4 Antibody [NBP1-33083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (NBP1-33083) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33083_rabbit-polyclonal-rfc4-antibody-910202420134095.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pT210 (panel A) and pan reactive anti-Plk-1 (panel B). Transfected cells were treated with 1 uM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and western blotting. Using a 1:1000 dilution, anti-Plk-1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0006.jpg)
![Western Blot: PLK1 [p Thr210] Antibody [NBP1-78021] - Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Western-Blot-NBP1-78021-img0002.jpg)
![Immunohistochemistry: PLK1 [p Thr210] Antibody [NBP1-78021] - Used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/PLK1-[p-Thr210]-Antibody-Immunohistochemistry-NBP1-78021-img0007.jpg)
![Western Blot: SIRT7 Antibody [NB100-56364] - Analysis of SIRT7 in A) human liver and B) PBMC lysate using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/SIRT7-Antibody-Western-Blot-NB100-56364-img0002.jpg)
![Immunohistochemistry-Paraffin: Senataxin Antibody (4G1) [NBP2-52726] - Senataxin antibody clone 4G1 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.](http://images.novusbio.com/fullsize/Senataxin-Antibody-4G1-Immunohistochemistry-Paraffin-NBP2-52726-img0002.jpg "Immunohistochemistry-Paraffin: Senataxin Antibody (4G1) [NBP2-52726] - Senataxin antibody clone 4G1 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.")
![Flow (Intracellular): Senataxin Antibody (4G1) [NBP2-52726] - An intracellular stain was performed on HepG2 cells with Senataxin Antibody (4G1) antibody NBP2-52726 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Senataxin-Antibody-4G1-Flow-Intracellular-NBP2-52726-img0001.jpg)
![Western Blot: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 Antibody [NBP2-16307] - 4EBP1 antibody detects 4EBP1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 antibody diluted at a dilution of 1:2000.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Western-Blot-NBP2-16307-img0006.jpg)
![Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4EBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-16307-img0003.jpg)
![Immunoprecipitation: 4EBP1 Antibody [NBP2-16307] - Immunoprecipitation of 4EBP1 protein from HCT116 whole cell extracts using 5 ug of 4EBP1 antibody or 4E-BP1 antibody. Western blot analysis was performed using 4EBP1 antibody.](http://images.novusbio.com/fullsize/4EBP1-Antibody-Immunoprecipitation-NBP2-16307-img0005.jpg)
![Western Blot: PRP19 Antibody [NBP1-31354] - A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP1-31354-img0015.jpg "Western Blot: PRP19 Antibody [NBP1-31354] - A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP1-31354] - Analysis of paraformaldehyde-fixed HeLa, using PRP19 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31354-img0008.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP1-31354] - Paraffin-embedded human cervical cancer. PRP19 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP1-31354-img0012.jpg)
![Western Blot: PRP19 Antibody [NBP1-31354] - A: mouse stomach 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP1-31354-img0010.jpg)
![Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Western-Blot-NB100-298-img0003.jpg "Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-298] - HeLa cells that were extracted for 5 min at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/SA1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-298-img0002.jpg)
![Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Western-Blot-NB100-56359-img0005.jpg "Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.")
![Immunohistochemistry-Paraffin: APE Antibody [NB100-56359] - Human Breast: FFPE tissue section using APE antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/APE-Antibody-Immunohistochemistry-Paraffin-NB100-56359-img0004.jpg)
![Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.](http://images.novusbio.com/fullsize/XPF-Antibody-Western-Blot-NB100-60679-img0004.jpg "Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.")
![Immunohistochemistry-Paraffin: XPF Antibody [NB100-60679] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- XPF/ERCC4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/XPF-Antibody-Immunohistochemistry-Paraffin-NB100-60679-img0007.jpg)
![Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/mL Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290C-Knockout-Validated-NBP2-61930-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/mL Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FANCD2 Antibody (1290C) [NBP2-61930] - Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line (Molecular weight: 164.1 KDa), Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Monoclonal FANCD2 Antibody (1290C) (Catalog # NBP2-61930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FANCD2-Antibody-1290C-Western-Blot-NBP2-61930-img0004.jpg)
![Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.](http://images.novusbio.com/fullsize/ATM-Antibody-Western-Blot-NB100-271-img0004.jpg "Western Blot: ATM Antibody [NB100-271] - Detection of human ATM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat ATM antibody [NB100-271] used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~360 kDa. Theoretical molecular weight 351 kDa.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ATM Antibody [NB100-271] - Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat ATM antibody [NB100-271] used for IP at 3 ug per reaction. ATM was also immunoprecipitated by another goat anti-ATM antibody from Company B. For blotting immunoprecipitated ATM, ATM antibody [NB100-271] was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATM-Antibody-Immunoprecipitation-NB100-271-img0005.jpg)
![Western Blot: FACA/FANCA Antibody [NB100-2564] - Detection of FANC A in transfected COS1 cell lysate.](http://images.novusbio.com/fullsize/FACA-FANCA-Antibody-Western-Blot-NB100-2564-img0004.jpg "Western Blot: FACA/FANCA Antibody [NB100-2564] - Detection of FANC A in transfected COS1 cell lysate.")