| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52518-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52565-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52549-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52590-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12658-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98634-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56526SS | |||
| Azide and BSA Free | NBP2-80974 | |||
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0004.jpg "Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0003.jpg)
![Flow Cytometry: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Flow-Cytometry-NBP2-52518-img0001.jpg)
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0002.jpg)
![ELISA: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-ELISA-NBP2-52518-img0006.jpg)
![Western Blot: SIRT7 Antibody (1E2G10) [NBP2-52565] - Analysis using SIRT7 mAb against human SIRT7 (AA: 1-105) recombinant protein. (Expected MW is 38 kDa)](http://images.novusbio.com/fullsize/SIRT7-Antibody-1E2G10-Western-Blot-NBP2-52565-img0003.jpg "Western Blot: SIRT7 Antibody (1E2G10) [NBP2-52565] - Analysis using SIRT7 mAb against human SIRT7 (AA: 1-105) recombinant protein. (Expected MW is 38 kDa)")
![Immunohistochemistry-Paraffin: SIRT7 Antibody (1E2G10) [NBP2-52565] - Analysis of paraffin-embedded ovarian cancer tissues using SIRT7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SIRT7-Antibody-1E2G10-Immunohistochemistry-Paraffin-NBP2-52565-img0002.jpg)
![Western Blot: SIRT7 Antibody (1E2G10) [NBP2-52565] - Analysis using SIRT7 mAb against HEK293 (1) and SIRT7 (AA: 1-105)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/SIRT7-Antibody-1E2G10-Western-Blot-NBP2-52565-img0004.jpg)
![Immunohistochemistry-Paraffin: SIRT7 Antibody (1E2G10) [NBP2-52565] - Analysis of paraffin-embedded liver cancer tissues using SIRT7 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SIRT7-Antibody-1E2G10-Immunohistochemistry-Paraffin-NBP2-52565-img0001.jpg)
![ELISA: SIRT7 Antibody (1E2G10) [NBP2-52565] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/SIRT7-Antibody-1E2G10-ELISA-NBP2-52565-img0005.jpg)
![Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0004.jpg "Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)")
![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded esophageal cancer tissues using RAD23A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0003.jpg)
![Flow Cytometry: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of HeLa cells using RAD23A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Flow-Cytometry-NBP2-52549-img0001.jpg)
![Western Blot: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis using RAD23A mAb against HEK293 (1) and RAD23A (AA: 1-363)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Western-Blot-NBP2-52549-img0005.jpg)
![Immunohistochemistry-Paraffin: Rad23 Antibody (3E3F4) [NBP2-52549] - Analysis of paraffin-embedded bladder cancer tissues using RAD23A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/hHR23A-Antibody-3E3F4-Immunohistochemistry-Paraffin-NBP2-52549-img0002.jpg)
![ELISA: Rad23 Antibody (3E3F4) [NBP2-52549] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Rad23-Antibody-3E3F4-ELISA-NBP2-52549-img0006.jpg)
![Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Western-Blot-NBP2-52590-img0003.jpg "Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)")
![Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Immunofluorescence-NBP2-52590-img0002.jpg)
![Flow Cytometry: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Flow-Cytometry-NBP2-52590-img0001.jpg)
![Western Blot: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-Western-Blot-NBP2-52590-img0004.jpg)
![ELISA: Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-ELISA-NBP2-52590-img0006.jpg)
![Ku70/XRCC6 Antibody (7A9E7) [NBP2-52590] - Analysis using XRCC6 mouse mAb against PC-2 (1), A549 (2), A431 (3), HepG2 (4), K562 (5) cell lysate.](http://images.novusbio.com/fullsize/Ku70-XRCC6-Antibody-7A9E7-NBP2-52590-img0007.jpg)
![Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution.<br/><br/> Lane 1: HL-60 cell lysate<br/> Lane 2: SiHa cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 102 kDa<br/> Observed band size: 130 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-27820241631683.jpg "Western Blot: MCM2 Antibody (PD00-90) [NBP3-32576] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/2,000 dilution.<br/><br/> Lane 1: HL-60 cell lysate<br/> Lane 2: SiHa cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 102 kDa<br/> Observed band size: 130 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32576) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/800 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32576) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-298202414262619.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (PD00-90) [NBP3-32576] - Immunocytochemistry analysis of LOVO cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32576) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241114811.jpg)
![Flow Cytometry: MCM2 Antibody (PD00-90) [NBP3-32576] - Flow cytometric analysis of HL-60 cells labeling MCM2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32576, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32576_rabbit-mcm2-mab-pd00-90-2920241637215.jpg)
![Western blot analysis on different lysates with NBP3-32576 at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lane 3: C2C12 (Mouse myoblast) cell lysate Lane 4: C6 (Rat glioma cell) cell lysate Lane 5: COS-1 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 11 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32576, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 102 kDa Observed band size: 125 kDa](http://images.novusbio.com/fullsize/nbp3-32576_-western-blot-639204594550797296.jpg)
![Western Blot: CC2D1A Antibody [NBP3-12658] - Lane A: Hela Whole Cell LysateLane B: HEK-293 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:130 kDa](http://images.novusbio.com/fullsize/CC2D1A-Antibody-Western-Blot-NBP3-12658-img0001.jpg "Western Blot: CC2D1A Antibody [NBP3-12658] - Lane A: Hela Whole Cell LysateLane B: HEK-293 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:130 kDa")
![Immunohistochemistry-Paraffin: CC2D1A Antibody [NBP3-12658] - Staining of human CC2D1A in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CC2D1A-Antibody-Immunohistochemistry-Paraffin-NBP3-12658-img0002.jpg)
![Immunoprecipitation: CC2D1A Antibody [NBP3-12658] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-CC2D1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CC2D1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 104 kDaObserved band size :130 kDa](http://images.novusbio.com/fullsize/CC2D1A-Antibody-Immunoprecipitation-NBP3-12658-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CC2D1A Antibody - BSA Free [NBP3-12658] - Immunofluorescence staining of CC2D1A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12658 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12658_rabbit-polyclonal-cc2d1a-antibody-235202514331558.png)
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg "Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western blot analysis on different lysates with NBP3-32228 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: Lane 1-7 (left): 40 seconds; Lane 1-7 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32228 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_-western-blot-639204597488042700.jpg)
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29820241426265.jpg "Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunocytochemistry analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A with Rabbit anti-p21/CIP1/CDKN1A/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202410334083.jpg)
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Flow cytometric analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202416372178.jpg)
![Western blot analysis on different lysates with NBP3-32691 at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32691 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32691_-western-blot-639204597484197336.jpg)
![Western Blot: hnRNP C1 + C2 Antibody [NBP2-98634] - Anti-hnRNP C1 + C2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Western-Blot-NBP2-98634-img0006.jpg "Western Blot: hnRNP C1 + C2 Antibody [NBP2-98634] - Anti-hnRNP C1 + C2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa")
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP2-98634] - Immunochemical staining of human hnRNP C1 + C2 in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-98634-img0005.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody [NBP2-98634] - Immunochemical staining of human hnRNP C1 + C2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-98634-img0004.jpg)
![Immunoprecipitation: hnRNP C1 + C2 Antibody [NBP2-98634] - hnRNP C1 + C2 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-hnRNP C1 + C2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-hnRNP C1 + C2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/hnRNP-C1-+-C2-Antibody-Immunoprecipitation-NBP2-98634-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody [NBP2-98634] - Immunofluorescence staining of hnRNP C1 + C2 in HEK293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98634 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-98634_rabbit-polyclonal-hnrnp-c1-c2-antibody-23520251129398.png)
![Immunohistochemistry: DMAP1 Antibody (JE55-31) [NBP3-32271] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32271) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-298202414262626.jpg "Immunohistochemistry: DMAP1 Antibody (JE55-31) [NBP3-32271] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32271) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: DMAP1 Antibody (JE55-31) [NBP3-32271] - Immunocytochemistry analysis of HeLa cells labeling DMAP1 with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DMAP1 antibody (NBP3-32271) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-292024119235.jpg)
![Flow Cytometry: DMAP1 Antibody (JE55-31) [NBP3-32271] - Flow cytometric analysis of HeLa cells labeling DMAP1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32271, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32271_rabbit-dmap1-mab-je55-31-392024884644.jpg)
![Western blot analysis on different lysates with NBP3-32271 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: F9 cell lysate Lane 10: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32271 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32271_-western-blot-639204597147676528.jpg)
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg "Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)
![Western blot analysis on different lysates with NBP3-32701 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: Lane 1-3: 59 seconds; Lane 4-7: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32701 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_-western-blot-639204597487461323.jpg)
![Western blot analysis on different lysates with NBP3-32701 at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with lambda pp for 1 hour Lysates/proteins at 15 µg/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32701 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_-western-blot-639204598086557790.jpg)
![Western Blot: TERF21P Antibody (78B356.1) [NB100-56526] - Western blot analysis of A431 (A), HEK293 (B) and HeLa (C) lysates (35ug per lane, RIPA buffer) using TERF21P antibody (NB100-56526) at 0.1ug/ml. Band observed at ~60kDa.](http://images.novusbio.com/fullsize/TERF21P-Antibody-78B356-1-Western-Blot-NB100-56526-img0003.jpg "Western Blot: TERF21P Antibody (78B356.1) [NB100-56526] - Western blot analysis of A431 (A), HEK293 (B) and HeLa (C) lysates (35ug per lane, RIPA buffer) using TERF21P antibody (NB100-56526) at 0.1ug/ml. Band observed at ~60kDa.")