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![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0006.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - (Top): Human osteosarcoma (U2OS) cells were stained with and DAPI. Cells were fixed with ice cold methanol for 10' and blocked with PBS/TX-100 containing 5% normal goat serum. Figure 6A: cells were immunofluorescently labeled with (left) diluted 1:300 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. (Bottom): Staining of the cells with the after incubation of the antibody with blocking peptide, concentration: 5 ng/ul.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59259-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - ChIP assays were performed using undifferentiated human teratocarcinoma cells (NCCIT), the antibody against H4K20me3 and optimized PCR primer sets for qPCR. Sheared chromatin from 10,000 cells was used per ChIP experiment. The antibody was tested at two different dilutions of 1:500 and 1:100. The pre-immune serum (PI) was used as a negative control. Quantitative PCR was performed using primer sets for the satellite repeat Sat2 as a positive control and for the promoter of the house keeping gene c-fos, as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H4K20me3 is preferably present at heterochromatin.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NBP2-59259-img0004.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone (acid) extracts of NB4 cells were analysed by Western blot using the Diagenode antibody against H4K20me3, diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0002.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Dot Blot was used to check the specificity of the antibody against H4K20me3 with peptides containing other modifications of histone H3 and H4. Other histone modifications include monoand dimethylation of the same lysine and mono-, di- and trimethylation of lysines 9, 27 and 36 of H3. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0001.jpg)
![ELISA: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H4K20me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:700.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-ELISA-NBP2-59259-img0003.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - A Dot Blot analysis was performed to test the cross reactivity of Histone H4 [Trimethyl Lys20] Antibody with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0007.jpg)
![Western Blot: PP2A alpha Antibody [NBP2-19896] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NBP2-19896-img0005.jpg "Western Blot: PP2A alpha Antibody [NBP2-19896] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PP2A alpha Antibody [NBP2-19896] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using PP2A alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19896-img0004.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NBP2-19896] - PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PP2A alpha stained by PP2A alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19896-img0007.jpg)
![Western Blot: PP2A alpha Antibody [NBP2-19896] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NBP2-19896-img0002.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NBP2-19896] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19896-img0003.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NBP2-19896] - PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PP2A alpha stained by PP2A alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19896-img0006.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - RIPA extract (30 ug) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). Antibody used at 0.33 or 0.1 ug/ml.](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0005.jpg)
![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0012.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0009.jpg "Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-33558] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-33558-img0003.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0004.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0005.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0008.jpg)
![Immunoprecipitation: Bub3 Antibody [NBP1-33558] - Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 ug of BUB3 antibody [N1C1]. Western blot analysis was performed using BUB3 antibody [N1C1]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunoprecipitation-NBP1-33558-img0007.jpg)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal CSB Antibody [NBP1-32405] - CSB antibody [N2C1] detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.Sample: Paraffin-embedded rat hind brain. CSB antibody [N2C1], dilution: 1:500.](http://images.novusbio.com/fullsize/nbp1-32405_rabbit-polyclonal-csb-antibody-immunohistochemistry-paraffin-1992023155353.jpg "Immunohistochemistry-Paraffin: Rabbit Polyclonal CSB Antibody [NBP1-32405] - CSB antibody [N2C1] detects CSB protein at nucleus on rat hind brain by immunohistochemical analysis.Sample: Paraffin-embedded rat hind brain. CSB antibody [N2C1], dilution: 1:500.")
![Immunohistochemistry-Paraffin: Rabbit Polyclonal CSB Antibody [NBP1-32405] - CSB antibody [N2C1] detects CSB protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain.CSB stained by CSB antibody [N2C1], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32405_rabbit-polyclonal-csb-antibody-immunohistochemistry-paraffin-1992023155617.jpg)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal CSB Antibody [NBP1-32405] - CSB antibody [N2C1] detects CSB protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CSB stained by CSB antibody [N2C1], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32405_rabbit-polyclonal-csb-antibody-immunohistochemistry-paraffin-199202316530..jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Polyclonal CSB Antibody [NBP1-32405] - CSB antibody [N2C1] detects CSB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CSB protein stained by CSB antibody [N2C1] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-32405_rabbit-polyclonal-csb-antibody-immunocytochemistry-immunofluorescence-199202316815..jpg)
![Immunohistochemistry-Paraffin: Rabbit Polyclonal CSB Antibody [NBP1-32405] - CSB antibody [N2C1] detects CSB protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CSB antibody [N2C1], dilution: 1:500. Antigen Retrieval: Trilogy (TM) (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-32405_rabbit-polyclonal-csb-antibody-immunohistochemistry-paraffin-1992023161059..jpg)
![Western Blot: CSRP3 Antibody [NBP1-32974] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CSRP3-Antibody-Western-Blot-NBP1-32974-img0006.jpg "Western Blot: CSRP3 Antibody [NBP1-32974] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CSRP3 Antibody [NBP1-32974] - Paraformaldehyde-fixed HeLa, using CSRP3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/CSRP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32974-img0002.jpg)
![Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-32974] - Mouse heart. CSRP3 stained by CSRP3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CSRP3-Antibody-Immunohistochemistry-Paraffin-NBP1-32974-img0009.jpg)
![Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-32974] - C2C12 xenograft. CSRP3 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CSRP3-Antibody-Immunohistochemistry-Paraffin-NBP1-32974-img0005.jpg)
![Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-32974] - Mouse muscle. CSRP3 stained by CSRP3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CSRP3-Antibody-Immunohistochemistry-Paraffin-NBP1-32974-img0010.jpg)
![Western Blot: CSRP3 Antibody [NBP1-32974] - Human heart (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-32974_rabbit-polyclonal-csrp3-antibody-western-blot-2011202385719..jpg)
![Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-159-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of mouse plasmacytoma Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-NB500-159-img0006.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-NB500-159-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 Antibody [NB500-159] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NB500-159 (lot NB500-159-4) used for IP at 6 ug per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-159-3) and rabbit anti-KAP-1 antibody NB500-158. For blotting immunoprecipitated KAP-1, NB500-159 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NB500-159-img0011.jpg)
![Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RFC4-Antibody-Western-Blot-NB100-233-img0054.jpg "<b>Biological Strategies Validation. </b>Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.](http://images.novusbio.com/fullsize/DMC1-Antibody-1D12-4-Western-Blot-NB100-181-img0003.jpg "Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.")
![Western Blot: USP7 Antibody [NBP2-24642] - analysis of USP7 protein in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NBP2-24642-img0001.jpg "Western Blot: USP7 Antibody [NBP2-24642] - analysis of USP7 protein in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")