| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90058-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-302SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-193SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-502SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22394SS | |||
| Azide and BSA Free | NBP2-80992 | |||
![Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Western-Blot-NBP2-22177-img0006.jpg "Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (5D11) [NBP2-22177] - Immunofluorescence analysis of GC-7901 cells using eIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunocytochemistry-Immunofluorescence-NBP2-22177-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using eIF4E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunohistochemistry-Paraffin-NBP2-22177-img0004.jpg)
![Flow Cytometry: eIF4E Antibody (5D11) [NBP2-22177] - Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Flow-Cytometry-NBP2-22177-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using eIF4E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunohistochemistry-Paraffin-NBP2-22177-img0003.jpg)
![ELISA: eIF4E Antibody (5D11) [NBP2-22177] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-ELISA-NBP2-22177-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0004.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200).](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0003.jpg)
![Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Western blot analysis of SGK1 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Western-Blot-NBP2-67561-img0009.jpg "Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Western blot analysis of SGK1 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0006.jpg)
![Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561] - Flow cytometric analysis of SGK1 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Flow-Cytometry-NBP2-67561-img0008.jpg)
![Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of SGK1 on human kidney lysates using anti-SGK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Western-Blot-NBP2-67561-img0007.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded human kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0002.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded human liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0003.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse brain tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0004.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0005.jpg)
![Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of 293 cells with SGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Flow-Cytometry-NBP2-67561-img0001.jpg)
![Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-302-img0003.jpg "Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.")
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-302] - The NTH1 antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-302-img0004.jpg)
![Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0015.jpg)
![Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0010.jpg)
![Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunoprecipitation-NB100-545-img0014.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)
![Western Blot: Rad9 Antibody [NB100-193] - Analysis of Rad9 on HeLa whole cell extracts using NB100-193.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NB100-193-img0007.jpg "Western Blot: Rad9 Antibody [NB100-193] - Analysis of Rad9 on HeLa whole cell extracts using NB100-193.")
![Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)](http://images.novusbio.com/fullsize/FANCD2-[p-Ser222]-Antibody-Western-Blot-NB100-502-img0007.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 [p Ser222] Antibody (C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E).](http://images.novusbio.com/fullsize/FANCD2-[p-Ser222]-Antibody-Western-Blot-NB100-502-img0006.jpg)
![Western Blot: XPC Antibody [NB100-58801] - Detection of Human XPC on HeLa whole cell lysate using NB100-58801. XPC was also immunoprecipitated by rabbit anti-XPC antibody NB100-58800.](http://images.novusbio.com/fullsize/XPC-Antibody-Western-Blot-NB100-58801-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: XPC Antibody [NB100-58801] - Detection of Human XPC on HeLa whole cell lysate using NB100-58801. XPC was also immunoprecipitated by rabbit anti-XPC antibody NB100-58800.")
![Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP2-36737-img0001.jpg "Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.")
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP2-36737] - Analysis of FFPE section of human testis using Aurora A antibody at 10 ug/ml concentration. Heat-induced antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP2-36737-img0002.jpg)
![Western Blot: TREX1 Antibody (41M5F2) [NBP2-22394] - Analysis of A) TREX1 partial recombinant protein (0.2 ug/ml) and B) human heart tissue (2 ug/ml) using TREX1 antibody.](http://images.novusbio.com/fullsize/TREX1-Antibody-41M5F2-Western-Blot-NBP2-22394-img0003.jpg "Western Blot: TREX1 Antibody (41M5F2) [NBP2-22394] - Analysis of A) TREX1 partial recombinant protein (0.2 ug/ml) and B) human heart tissue (2 ug/ml) using TREX1 antibody.")
![Immunohistochemistry-Paraffin: TREX1 Antibody (41M5F2) [NBP2-22394] - Human spleen stained with TREX1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/TREX1-Antibody-41M5F2-Immunohistochemistry-Paraffin-NBP2-22394-img0004.jpg)
![Flow Cytometry: TREX1 Antibody (41M5F2) [NBP2-22394] - An intracellular stain was performed on HeLa cells with TREX1 [41M5F2] Antibody NBP2-22394AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/TREX1-Antibody-41M5F2-Flow-Cytometry-NBP2-22394-img0005.jpg)