Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-5220251733576.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (9D6F7) [p53] - Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb at 1:59000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![<b>Genetic Strategies Validation. </b>Immunoprecipitation: p53 Antibody (9D6F7) [p53] - Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb . Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15792_rabbit-p53-mab-9d6f7-52202517344284.jpg)
![Western Blot: SFPQ Antibody (B92) - BSA Free [NB120-11825] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-PSF Clone: B92. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HeLa 2. HepG2 3. A431 4. HUVEC 5. NCI-H1299 6. WiDr 7. Hepa 1-6 8. MM3MG](http://images.novusbio.com/fullsize/SFPQ-Antibody-B92-BSA-Free-Western-Blot-NB120-11825-img0003.jpg "Western Blot: SFPQ Antibody (B92) - BSA Free [NB120-11825] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-PSF Clone: B92. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HeLa 2. HepG2 3. A431 4. HUVEC 5. NCI-H1299 6. WiDr 7. Hepa 1-6 8. MM3MG")
![Western Blot: MSH6 Antibody (PD00-26) [NBP3-32607] - Western blot analysis of MSH6 on different lysates with Rabbit anti-MSH6 antibody (NBP3-32607) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 153 kDa Observed band size: 153 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32607) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32607_rabbit-msh6-mab-pd00-26-278202415242625.jpg "Western Blot: MSH6 Antibody (PD00-26) [NBP3-32607] - Western blot analysis of MSH6 on different lysates with Rabbit anti-MSH6 antibody (NBP3-32607) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 153 kDa Observed band size: 153 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32607) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MSH6 Antibody (PD00-26) [NBP3-32607] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MSH6 antibody (NBP3-32607) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32607) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32607_rabbit-msh6-mab-pd00-26-2982024143521.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-RFB4-Flow-Cytometry-NBP2-49890-img0001.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (RFB4) [NBP2-49890] - Flow Cytometry using Siglec-2/CD22 Antibody (RFB4).")
![Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-278202416285244.jpg "Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MLH1 Antibody (PD01-61) [NBP3-32599] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-298202413585516.jpg)
![Western Blot: ASC1 Antibody (PSH0-26) [NBP3-32049] - Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: U87-MG cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: HepG2 cell lysate Lane 7: RH-35 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32049) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32049_rabbit-asc1-mab-psh0-26-278202415212424.jpg "Western Blot: ASC1 Antibody (PSH0-26) [NBP3-32049] - Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: U87-MG cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: HepG2 cell lysate Lane 7: RH-35 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32049) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ASC1 Antibody (PSH0-26) [NBP3-32049] - Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32049) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32049_rabbit-asc1-mab-psh0-26-298202413585548.jpg)
![Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Hs-8-Flow-Cytometry-NB500-456-img0001.jpg "Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.")
![Western Blot: MSH6 Antibody (A4C4-R) [NBP3-32606] - Western blot analysis of MSH6 on different lysates with Mouse anti-MSH6 antibody (NBP3-32606) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 153 kDa Observed band size: 180/140 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32606) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32606_mouse-msh6-mab-a4c4-r-278202415242631.jpg "Western Blot: MSH6 Antibody (A4C4-R) [NBP3-32606] - Western blot analysis of MSH6 on different lysates with Mouse anti-MSH6 antibody (NBP3-32606) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 153 kDa Observed band size: 180/140 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32606) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MSH6 Antibody (A4C4-R) [NBP3-32606] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-MSH6 antibody (NBP3-32606) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32606) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32606_mouse-msh6-mab-a4c4-r-29820241420965.jpg)
![Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-278202416331276.jpg "Western Blot: p53 Antibody (6B2-R) [NBP3-32700] - Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (NBP3-32700) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: A431 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B2-R) [NBP3-32700] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32700) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32700_mouse-p53-mab-6b2-r-29202410234254.jpg)
![Dot Blot: 5-hmC Antibody (C.15200200) [NBP2-59177] - 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - Dot Blot analysis of the antibody with the C, mC and hmC PCR controls 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the antibody (dilution 1:500). The membranes were exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15200200-Dot-Blot-NBP2-59177-img0001.jpg "Dot Blot: 5-hmC Antibody (C.15200200) [NBP2-59177] - 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - Dot Blot analysis of the antibody with the C, mC and hmC PCR controls 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the antibody (dilution 1:500). The membranes were exposed for 30 seconds.")
![Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15200200) [NBP2-59177] - 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - An hydroxymethylated DNA IP (hMeDIP) was performed using the antibody directed against 5-hydroxymethylcytosine. The IgG isotype antibodies from mouse was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. 1 ug of human HeLa cells DNA were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The IP'd material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the mouse monoclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments).](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-C-15200200-Methylated-DNA-Immunoprecipitation-NBP2-59177-img0002.jpg)
![ELISA: 5-hmC Antibody (C.15200200) [NBP2-59177] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.](http://images.novusbio.com/fullsize/5-hmC-Antibody-C-15200200-ELISA-NBP2-59177-img0004.jpg)
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401] - Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-DCS-72-F6-+-KIP1-769-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48401-img0001.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) - IHC-Prediluted [NBP2-48401] - Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6 + KIP1/769)")