| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89858-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00348-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11971-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56360SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59614-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24808SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56685SS | |||
![Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-278202415212410.jpg "Western Blot: Nucleolin Antibody (JE65-71) [NBP3-32668] - Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/5,000 dilution. Lane 1: K562 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 77 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32668) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32668) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-298202414333643.jpg)
![Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (JE65-71) [NBP3-32668] - Immunocytochemistry analysis of Hela cells labeling Nucleolin with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nucleolin antibody (NBP3-32668) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32668_rabbit-nucleolin-mab-je65-71-29202410334079.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Anti-PP2C gamma/PPM1g rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Western-Blot-NBP2-89858-img0005.jpg "Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Anti-PP2C gamma/PPM1g rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa.")
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of human PPM1G in human epididymis with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Immunohistochemistry-Paraffin-NBP2-89858-img0002.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of human PPM1G in human heart with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Immunohistochemistry-Paraffin-NBP2-89858-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of Human PPM1G in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89858 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/nbp2-89858_rabbit-monoclonal-pp2c-gamma-ppm1g-antibody-112-23520251129355.png)
![Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-2352025162819.png "Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa")
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human placenta with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545641.png)
![Western Blot: MRTO4 Antibody [NBP3-00348] - NBP3-00348 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-23520251664731.png)
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human liver with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545651.png)
![Flow Cytometry: Podoplanin Antibody (PMab-1) - Chimeric [NBP3-11971] - NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (NBP3-11971, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-PMab-1-Chimeric-Flow-Cytometry-NBP3-11971-img0001.jpg "Flow Cytometry: Podoplanin Antibody (PMab-1) - Chimeric [NBP3-11971] - NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (NBP3-11971, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Podoplanin (MAP tag) expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP3-11971 at 0.00001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-11971_rabbit-monoclonal-podoplanin-antibody-pmab-1-chimeric-74202522113027.png)
![Immunocytochemistry /Immunofluorescence: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with Podoplanin (MAP tag) expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP3-11971 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-11971_rabbit-monoclonal-podoplanin-antibody-pmab-1-chimeric-74202522113019.png)
![Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-11971 at 0.01 ug/ml, 0.1 ug/ml, 0.001 ug/ml and 0.003 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-11971_rabbit-monoclonal-podoplanin-antibody-pmab-1-chimeric-74202522141240.png)
![Western Blot: UEV2 Antibody [NB100-56360] - Detection of UBE2V2 in HL-60 cell lysate with anti-UBE2V2 pcAb this antibody at 2ug/ml.](http://images.novusbio.com/fullsize/UEV2-Antibody-Western-Blot-NB100-56360-img0002.jpg "Western Blot: UEV2 Antibody [NB100-56360] - Detection of UBE2V2 in HL-60 cell lysate with anti-UBE2V2 pcAb this antibody at 2ug/ml.")
![Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Western-Blot-NBP2-59613-img0004.jpg "Western Blot: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (MCM7/1469).")
![Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Immunohistochemistry-Paraffin-NBP2-59613-img0001.jpg)
![Protein Array: MCM7 Antibody (MCM7/1469) [NBP2-59613] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Mouse Monoclonal Antibody (MCM7/1469).](http://images.novusbio.com/fullsize/MCM7-Antibody-MCM7-1469-Protein-Array-NBP2-59613-img0005.jpg)
![Western Blot: PUS1 Antibody [NBP1-32902] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PUS1-Antibody-Western-Blot-NBP1-32902-img0005.jpg "Western Blot: PUS1 Antibody [NBP1-32902] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PUS1 Antibody [NBP1-32902] - Paraffin-embedded mouse muscle. PUS1 antibody [N2C3] diluted at 1:500.](http://images.novusbio.com/fullsize/PUS1-Antibody-Immunohistochemistry-Paraffin-NBP1-32902-img0006.jpg)
![Western Blot: PUS1 Antibody [NBP1-32902] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PUS1-Antibody-Western-Blot-NBP1-32902-img0004.jpg)
![Western Blot: PSMA7 Antibody [NBP1-33539] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA7 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PSMA7-Antibody-Western-Blot-NBP1-33539-img0006.jpg "Western Blot: PSMA7 Antibody [NBP1-33539] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA7 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PSMA7 Antibody [NBP1-33539] - Paraffin-embedded DLD-1 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PSMA7-Antibody-Immunohistochemistry-Paraffin-NBP1-33539-img0003.jpg)
![Western Blot: PSMA7 Antibody [NBP1-33539] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PSMA7-Antibody-Western-Blot-NBP1-33539-img0004.jpg)
![Western Blot: PSMA7 Antibody [NBP1-33539] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PSMA7-Antibody-Western-Blot-NBP1-33539-img0005.jpg)
![Western Blot: Sars Spike Protein Antibody [NB100-56685] - analysis of SARS-Spike protein in full-length (A) transfected and (B) untransfected mouse melanoma cell lysate using this antibody.](http://images.novusbio.com/fullsize/Sars-Spike-Protein-Antibody-Western-Blot-NB100-56685-img0002.jpg "Western Blot: Sars Spike Protein Antibody [NB100-56685] - analysis of SARS-Spike protein in full-length (A) transfected and (B) untransfected mouse melanoma cell lysate using this antibody.")
![Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP2-17296] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Mitochondrial ribosomal protein L11 Antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Mitochondrial-ribosomal-protein-L11-Antibody-Western-Blot-NBP2-17296-img0003.jpg "Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP2-17296] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Mitochondrial ribosomal protein L11 Antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Mitochondrial ribosomal protein L11 Antibody [NBP2-17296] - Paraffin-embedded lung carcinoma. MRPL11 antibody [N1C3] dilution: 1:250.](http://images.novusbio.com/fullsize/Mitochondrial-ribosomal-protein-L11-Antibody-Immunohistochemistry-Paraffin-NBP2-17296-img0002.jpg)
![Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP2-17296] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Mitochondrial-ribosomal-protein-L11-Antibody-Western-Blot-NBP2-17296-img0001.jpg)
![Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Western-Blot-NBP1-18878-img0004.jpg "Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.")
![Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP1-18878] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-18878-img0003.jpg)
![Immunoprecipitation: Cyclin C Antibody [NBP1-18878] - Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody NBP1-18878 used for IP at 6 ug per reaction. For blotting immunoprecipitated Cyclin C, NBP1-18878 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Cyclin-C-Antibody-Immunoprecipitation-NBP1-18878-img0005.jpg)