| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99843-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89858-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06479-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90208-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00348-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06333-50ul | |||
![Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-278202415212451.jpg "Western Blot: APE Antibody (12H2-R) [NBP3-32043] - Western blot analysis of APE on different lysates with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: LNCaP cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HL-60 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: APE Antibody (12H2-R) [NBP3-32043] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-APE antibody (NBP3-32043) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32043) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-298202414333696.jpg)
![Immunocytochemistry/ Immunofluorescence: APE Antibody (12H2-R) [NBP3-32043] - Immunocytochemistry analysis of A549 cells labeling APE with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-APE antibody (NBP3-32043) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32043_mouse-ape-mab-12h2-r-2920241192367.jpg)
![Western Blot: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Western blot analysis of RNA Helicase A on different lysates with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: HEK-293 cell lysate (15 ug/Lane) Lane 5: K-562 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: PANC-1 cell lysate (15 ug/Lane) Lane 8: MCF7 cell lysate (15 ug/Lane) Lane 9: Human brain tissue lysate (30 ug/Lane) Predicted band size: 141 kDa Observed band size: 141 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32907) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-288202412442931.jpg "Western Blot: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Western blot analysis of RNA Helicase A on different lysates with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (15 ug/Lane) Lane 2: A431 cell lysate (15 ug/Lane) Lane 3: HeLa cell lysate (15 ug/Lane) Lane 4: HEK-293 cell lysate (15 ug/Lane) Lane 5: K-562 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: PANC-1 cell lysate (15 ug/Lane) Lane 8: MCF7 cell lysate (15 ug/Lane) Lane 9: Human brain tissue lysate (30 ug/Lane) Predicted band size: 141 kDa Observed band size: 141 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32907) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32907) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-29820241411541.jpg)
![Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody (JE34-97) [NBP3-32907] - Immunocytochemistry analysis of HeLa cells labeling RNA Helicase A with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RNA Helicase A antibody (NBP3-32907) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32907_rabbit-rna-helicase-a-mab-je34-97-29202410484888.jpg)
![Immunohistochemistry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32422) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-298202414262699.jpg "Immunohistochemistry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32422) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunocytochemistry analysis of MCF-7 cells labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-29202410234277.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Flow cytometric analysis of SK-Br-3 cells labeling ErbB2/Her2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32422, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-29202416453290.jpg)
![Western Blot: ALDH7A1 Antibody [NBP2-99843] - Anti-ALDH7A1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: SH-SY5Y Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/ALDH7A1-Antibody-Western-Blot-NBP2-99843-img0006.jpg "Western Blot: ALDH7A1 Antibody [NBP2-99843] - Anti-ALDH7A1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: SH-SY5Y Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: ALDH7A1 Antibody [NBP2-99843] - Immunochemical staining of human ALDH7A1 in human liver (from 2 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections. The image showing positive staining of hepatocyte.](http://images.novusbio.com/fullsize/ALDH7A1-Antibody-Immunohistochemistry-Paraffin-NBP2-99843-img0004.jpg)
![Immunohistochemistry-Paraffin: ALDH7A1 Antibody [NBP2-99843] - Immunochemical staining of human ALDH7A1 in human hepatoma (from 3 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ALDH7A1-Antibody-Immunohistochemistry-Paraffin-NBP2-99843-img0003.jpg)
![Immunoprecipitation: ALDH7A1 Antibody [NBP2-99843] - ALDH7A1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 0.5 ul anti-ALDH7A1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ALDH7A1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa](http://images.novusbio.com/fullsize/ALDH7A1-Antibody-Immunoprecipitation-NBP2-99843-img0005.jpg)
![Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Anti-PP2C gamma/PPM1g rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Western-Blot-NBP2-89858-img0005.jpg "Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Anti-PP2C gamma/PPM1g rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa.")
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of human PPM1G in human epididymis with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Immunohistochemistry-Paraffin-NBP2-89858-img0002.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of human PPM1G in human heart with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-112-Immunohistochemistry-Paraffin-NBP2-89858-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of Human PPM1G in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89858 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).](http://images.novusbio.com/fullsize/nbp2-89858_rabbit-monoclonal-pp2c-gamma-ppm1g-antibody-112-23520251129355.png)
![Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-278202416285221.jpg "Western Blot: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Western blot analysis of p57 Kip2 on different lysates with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50nM dexamethasone for 16 hours cell lysate Lane 3: HeLa treated with 50nM dexamethasone for 16 hours cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 15 ug/Lane. Predicted band size: 32 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32701) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29820241420942.jpg)
![Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody (JE02-08) [NBP3-32701] - Immunocytochemistry analysis of HeLa cells treated with or without 50nM dexamethasone for 16 hours labeling p57 Kip2 with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p57 Kip2 antibody (NBP3-32701) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32701_rabbit-p57-kip2-mab-je02-08-29202410484891.jpg)
![Western Blot: UDP glucose dehydrogenase Antibody [NBP3-06479] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:57 kDa](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Western-Blot-NBP3-06479-img0001.jpg "Western Blot: UDP glucose dehydrogenase Antibody [NBP3-06479] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:57 kDa")
![Immunohistochemistry-Paraffin: UDP glucose dehydrogenase Antibody [NBP3-06479] - Staining of human UGDH in human prostate with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP3-06479-img0003.jpg)
![Immunohistochemistry-Paraffin: UDP glucose dehydrogenase Antibody [NBP3-06479] - Staining of human UGDH in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP3-06479-img0002.jpg)
![Immunoprecipitation: UDP glucose dehydrogenase Antibody [NBP3-06479] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-UGDH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-UGDH rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :57 kDa](http://images.novusbio.com/fullsize/UDP-glucose-dehydrogenase-Antibody-Immunoprecipitation-NBP3-06479-img0004.jpg)
![Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Flow-Cytometry-NBP2-90208-img0003.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t")
![<b>Biological Strategies Validation. </b>Western Blot: ERp72 Antibody (020) [NBP2-90208] - Anti-PDIA4 rabbit monoclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Western-Blot-NBP2-90208-img0006.jpg)
![Immunoprecipitation: ERp72 Antibody (020) [NBP2-90208] - PDIA4 was immunoprecipitated using: Lane A :0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg MCF-7 Whole Cell Lysate. 2 uL anti-PDIA4 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:](http://images.novusbio.com/fullsize/ERp72-Antibody-020-Immunoprecipitation-NBP2-90208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ERp72 Antibody (020) [NBP2-90208] - Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90208 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90208_rabbit-monoclonal-erp72-antibody-020-235202511545653.png)
![Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-2352025162819.png "Immunoprecipitation: MRTO4 Antibody [NBP3-00348] - Mouse RTO4 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00348 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00348, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa")
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human placenta with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545641.png)
![Western Blot: MRTO4 Antibody [NBP3-00348] - NBP3-00348 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-23520251664731.png)
![Immunohistochemistry-Paraffin: MRTO4 Antibody [NBP3-00348] - Immunochemical staining of human MRTO4 in human liver with NBP3-00348 (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00348_rabbit-polyclonal-mrto4-antibody-235202511545651.png)
![Western Blot: KIF20A Antibody [NBP2-87689] - KIF20A antibody - middle region validated by WB using 721_B Cell Lysate at 1ug/ml. KIF20A is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/KIF20A-Antibody-Western-Blot-NBP2-87689-img0005.jpg "Western Blot: KIF20A Antibody [NBP2-87689] - KIF20A antibody - middle region validated by WB using 721_B Cell Lysate at 1ug/ml. KIF20A is supported by BioGPS gene expression data to be expressed in 721_B")
![Immunohistochemistry: KIF20A Antibody [NBP2-87689] - Immunohistochemistry with Human Thyroid lysate tissue at an antibody concentration of 5.0ug/ml using anti-KIF20A antibody](http://images.novusbio.com/fullsize/KIF20A-Antibody-Immunohistochemistry-NBP2-87689-img0004.jpg)
![Immunohistochemistry: KIF20A Antibody [NBP2-87689] - Immunohistochemistry with HeLa, Vera, HeLa transfected with mouse construct tissue](http://images.novusbio.com/fullsize/KIF20A-Antibody-Immunohistochemistry-NBP2-87689-img0001.jpg)
![Immunohistochemistry: KIF20A Antibody [NBP2-87689] - Immunohistochemistry with HeLa, Vera, HeLa transfected with mouse construct tissue](http://images.novusbio.com/fullsize/KIF20A-Antibody-Immunohistochemistry-NBP2-87689-img0002.jpg)
![Immunohistochemistry: KIF20A Antibody [NBP2-87689] - Immunohistochemistry with HeLa, Vera, HeLa transfected with mouse construct tissue](http://images.novusbio.com/fullsize/KIF20A-Antibody-Immunohistochemistry-NBP2-87689-img0003.jpg)
![Western Blot: MSH2 Antibody (10G3-R) [NBP3-32604] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32604) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate, 10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: A431 cell lysate, 10 ug/Lane Lane 4: NIH-3T3 cell lysate, 10 ug/Lane Lane 5: Mouse testis tissue lysate, 20 ug/Lane Lane 6: Mouse heart tissue lysate, 20 ug/Lane Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 5% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32604) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32604_mouse-msh2-mab-10g3-r-27820241631696.jpg "Western Blot: MSH2 Antibody (10G3-R) [NBP3-32604] - Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (NBP3-32604) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate, 10 ug/Lane Lane 2: PC-12 cell lysate, 10 ug/Lane Lane 3: A431 cell lysate, 10 ug/Lane Lane 4: NIH-3T3 cell lysate, 10 ug/Lane Lane 5: Mouse testis tissue lysate, 20 ug/Lane Lane 6: Mouse heart tissue lysate, 20 ug/Lane Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 5% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32604) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MSH2 Antibody (10G3-R) [NBP3-32604] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MSH2 antibody (NBP3-32604) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32604) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32604_mouse-msh2-mab-10g3-r-298202414304163.jpg)
![Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (10G3-R) [NBP3-32604] - Immunocytochemistry analysis of Daudi cells labeling MSH2 with Mouse anti-MSH2 antibody (NBP3-32604) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MSH2 antibody (NBP3-32604) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32604_mouse-msh2-mab-10g3-r-29202410234279.jpg)
![Western Blot: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Western-Blot-NBP3-06333-img0001.jpg "Western Blot: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa")
![Immunoprecipitation: integrin beta 4 binding protein Antibody [NBP3-06333] - Integrin beta 4 binding protein Antibody was immunoprecipitated using:Lane A: 0.5 mg Hela Whole Cell Lysate4 uL anti-EIF6 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-EIF6 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 26 kDa](http://images.novusbio.com/fullsize/integrin-beta-4-binding-protein-Antibody-Immunoprecipitation-NBP3-06333-img0002.jpg)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody - BSA Free [NBP3-06333] - Immunochemical staining of human integrin beta 4 binding protein in human adrenal gland with NBP3-06333 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06333_rabbit-polyclonal-integrin-beta-4-binding-protein-antibody-23520251664728.png)
![Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody - BSA Free [NBP3-06333] - Immunochemical staining of human integrin beta 4 binding protein in human esophagus with NBP3-06333 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06333_rabbit-polyclonal-integrin-beta-4-binding-protein-antibody-23520251129341.png)