| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09017-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26371-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22832SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54347-100ug | |||
![Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Western-Blot-NBP3-09017-img0003.jpg "Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: MHC Class I Antibody (R1-21.2) - Chimeric - Azide and BSA Free [NBP3-09017] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized and stained with NBP3-09017 at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09017, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-09017_rabbit-monoclonal-mhc-class-i-antibody-r1-21-2-chimeric-74202522265217.png)
![Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-262202414471544.jpg "Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-262202414471550.jpg)
![Western Blot: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Positive Western Blot detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: Peroxiredoxin 3 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28, 26 kDa Observed band size: 20-28 kDa](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-282202411442649.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Staining of PC-3 cell with Peroxiredoxin 3 Antibody (14E2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 503-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-2822024131224.jpg)
![Flow Cytometry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Overlay Peak curve showing HepG2 cells stained with Peroxiredoxin 3 Antibody (14E2) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26371_rabbit-peroxiredoxin-3-mab-14e2-28220241431275.jpg)
![Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Western-Blot-NBP2-19397-img0002.jpg "Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-19397] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19397-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody [NBP2-19397] - MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-19397_rabbit-polyclonal-mrpl12-antibody-91020242091420.jpg)
![Western Blot: MRPL12 Antibody [NBP2-19397] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19397_rabbit-polyclonal-mrpl12-antibody-91020242010446.jpg)
![Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-375-img0005.jpg "Western Blot: SMC4 Antibody [NB100-375] - Whole cell lysate (90 mcg for WB; 0.5 mg for IP/WB) from 293T cells (lanes 1, 3 and 5), normal human lymphoblasts (lane 2) or SMC4 transfected 239T cells (lanes 4, 6 and 7) separated on an 8% SDS-PAGE gel. Affinity purified rabbit anti-SMC4 used at 0.2 or 0.1 mcg/ml for WB and 5 mcg for IP.")
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded mouse kidney. Hsp47 antibody Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP2-16893-img0003.jpg "Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded mouse kidney. Hsp47 antibody Internal dilution: 1:500.")
![Western Blot: Hsp47 Antibody [NBP2-16893] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP2-16893-img0001.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded human breast carcinoma. Hsp47 antibody Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP2-16893-img0004.jpg)
![HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract10% SDS-PAGEHSP47 antibody [N2C2], Internal (NBP2-16893) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16893_rabbit-polyclonal-hsp47-antibody-western-blot-261120251847574.jpg)
![HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.A. 30 ug A431 whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract10% SDS-PAGEHSP47 antibody [N2C2], Internal (NBP2-16893) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16893_rabbit-polyclonal-hsp47-antibody-western-blot-261120251818916.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFB10 Antibody [NBP2-19503] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NDUFB10-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19503-img0003.jpg "Immunocytochemistry/Immunofluorescence: NDUFB10 Antibody [NBP2-19503] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: NDUFB10 Antibody [NBP2-19503] - Human breast cancer, using NDUFB10 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/NDUFB10-Antibody-Immunohistochemistry-Paraffin-NBP2-19503-img0002.jpg)
![Western Blot: NDUFB10 Antibody [NBP2-19503] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (NBP2-19503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-19503_rabbit-polyclonal-ndufb10-antibody-910202420115070.jpg)
![Western Blot: NDUFB10 Antibody [NBP2-19503] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (NBP2-19503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-19503_rabbit-polyclonal-ndufb10-antibody-910202420134068.jpg)
![Western Blot: CRABP2 Antibody [NB100-2590] - Whole cell lysate (5, 15 and 50 ug) from MCF7 cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NB100-2590-img0007.jpg "Western Blot: CRABP2 Antibody [NB100-2590] - Whole cell lysate (5, 15 and 50 ug) from MCF7 cells. Antibody used at 0.04 ug/ml.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NB100-2590] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CRABP2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NB100-2590-img0009.jpg)
![Western Blot: Cyclin B2 Antibody [NBP1-33031] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Western-Blot-NBP1-33031-img0004.jpg "Western Blot: Cyclin B2 Antibody [NBP1-33031] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031] - Cyclin B2 Antibody detects Cyclin B2 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Cyclin B2 Antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-33031-img0005.jpg)
![Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP1-33031] - Paraffin-embedded H1299 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Cyclin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-33031-img0003.jpg)
![Cyclin B2 antibody [N1C3-2] detects CCNB2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: CCNB2 protein stained by Cyclin B2 antibody [N1C3-2] (NBP1-33031) diluted at 1:500.Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/nbp1-33031_rabbit-polyclonal-cyclin-b2-antibody-immunocytochemistry-immunofluorescence-2611202518393122.jpg)
![Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Western-Blot-NBP3-15177-img0005.jpg "Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: hnRNP K Antibody (3G7) [NBP3-15177] - Immunofluorescence staining of A549 cells with at hnRNP K antibody 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunocytochemistry-Immunofluorescence-NBP3-15177-img0001.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0004.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0003.jpg)
![Immunoprecipitation: hnRNP K Antibody (3G7) [NBP3-15177] - Immunoprecipitating hnRNP K in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of hnRNP K antibody in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: hnRNP K antibody (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunoprecipitation-NBP3-15177-img0002.jpg)
![Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-22832-img0004.jpg "Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.")
![Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/1433) [NBP2-53292] - Formalin-fixed, paraffin-embedded human Cervix stained with Podoplanin Monoclonal Antibody (PDPN/1433)](http://images.novusbio.com/fullsize/Podoplanin-Antibody-PDPN-1433-Immunohistochemistry-Paraffin-NBP2-53292-img0001.jpg)
![Protein Array: Podoplanin Antibody (PDPN/1433) [NBP2-53292] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Podoplanin Antibody (PDPN/1433) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-PDPN-1433-Protein-Array-NBP2-53292-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.](http://images.novusbio.com/fullsize/Chk2-Antibody-8F12-Immunocytochemistry-Immunofluorescence-NB100-500-img0003.jpg "Immunocytochemistry/Immunofluorescence: Chk2 Antibody (8F12) [NB100-500] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 1:500 dilution.")
![Western Blot: Chk2 Antibody (8F12) [NB100-500] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (NB100-500) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-500_mouse-monoclonal-chk2-antibody-8f12-91020242014681.jpg)
