| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06045-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09017-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56394SS | |||
![Western Blot: MRPS2 Antibody [NBP3-06045] - Anti-MRPS2 rabbit polyclonal antibody at 1:500 dilution Lane A: A-431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:33 kDa](http://images.novusbio.com/fullsize/MRPS2-Antibody-Western-Blot-NBP3-06045-img0001.jpg "Western Blot: MRPS2 Antibody [NBP3-06045] - Anti-MRPS2 rabbit polyclonal antibody at 1:500 dilution Lane A: A-431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:33 kDa")
![Immunohistochemistry-Paraffin: MRPS2 Antibody [NBP3-06045] - Immunochemical staining of human MRPS2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MRPS2-Antibody-Immunohistochemistry-Paraffin-NBP3-06045-img0003.jpg)
![Immunoprecipitation: MRPS2 Antibody [NBP3-06045] - MRPS2 was immunoprecipitated using: Lane A:0.5 mg A-431 Whole Cell Lysate 4 uL anti-MRPS2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-MRPS2 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa Observed band size :33 kDa](http://images.novusbio.com/fullsize/MRPS2-Antibody-Immunoprecipitation-NBP3-06045-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MRPS2 Antibody [NBP3-06045] - Immunofluorescence staining of MRPS2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06045 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06045_rabbit-polyclonal-mrps2-antibody-235202514492343.png)
![Western Blot: G3BP1 Antibody (JE35-20) [NBP3-32358] - Western blot analysis of G3BP1 on different lysates with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: N2A cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32358) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-278202416285287.png "Western Blot: G3BP1 Antibody (JE35-20) [NBP3-32358] - Western blot analysis of G3BP1 on different lysates with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: N2A cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 68 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32358) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: G3BP1 Antibody (JE35-20) [NBP3-32358] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32358) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-298202414161887.jpg)
![Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (JE35-20) [NBP3-32358] - Immunocytochemistry analysis of LOVO cells labeling G3BP1 with Rabbit anti-G3BP11 antibody (NBP3-32358) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-G3BP1 antibody (NBP3-32358) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-29202410334082.jpg)
![Flow Cytometry: G3BP1 Antibody (JE35-20) [NBP3-32358] - Flow cytometric analysis of A549 cells labeling G3BP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32358, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32358_rabbit-g3bp1-mab-je35-20-29202416453267.png)
![Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Western-Blot-NBP3-09017-img0003.jpg "Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: MHC Class I Antibody (R1-21.2) - Chimeric - Azide and BSA Free [NBP3-09017] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized and stained with NBP3-09017 at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09017, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-09017_rabbit-monoclonal-mhc-class-i-antibody-r1-21-2-chimeric-74202522265217.png)
![Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg)
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Western Blot: CRABP2 Antibody [NB100-2590] - Whole cell lysate (5, 15 and 50 ug) from MCF7 cells. Antibody used at 0.04 ug/ml.](http://images.novusbio.com/fullsize/CRABP2-Antibody-Western-Blot-NB100-2590-img0007.jpg "Western Blot: CRABP2 Antibody [NB100-2590] - Whole cell lysate (5, 15 and 50 ug) from MCF7 cells. Antibody used at 0.04 ug/ml.")
![Immunohistochemistry-Paraffin: CRABP2 Antibody [NB100-2590] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CRABP2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CRABP2-Antibody-Immunohistochemistry-Paraffin-NB100-2590-img0009.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0002.jpg "Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EBP1 Antibody [NBP2-16257] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16257-img0003.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0001.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP2-16257 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-91020242021926.jpg)
![Immunohistochemistry: PA2G4/EBP1 Antibody [NBP2-16257] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PA2G4/EBP1 antibody [N3C3] (NBP2-16257) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-9102024201579.jpg)
![Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Western-Blot-NBP3-15177-img0005.jpg "Western Blot: hnRNP K Antibody (3G7) [NBP3-15177] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: hnRNP K antibody at 1.3ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 52, 49 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: hnRNP K Antibody (3G7) [NBP3-15177] - Immunofluorescence staining of A549 cells with at hnRNP K antibody 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunocytochemistry-Immunofluorescence-NBP3-15177-img0001.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0004.jpg)
![Immunohistochemistry: hnRNP K Antibody (3G7) [NBP3-15177] - IHC image of hnRNP K antibody diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunohistochemistry-NBP3-15177-img0003.jpg)
![Immunoprecipitation: hnRNP K Antibody (3G7) [NBP3-15177] - Immunoprecipitating hnRNP K in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of hnRNP K antibody in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: hnRNP K antibody (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-3G7-Immunoprecipitation-NBP3-15177-img0002.jpg)
![Western Blot: CHAF1A Antibody [NB100-74609] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caf1p150 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74609-img0003.jpg "Western Blot: CHAF1A Antibody [NB100-74609] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caf1p150 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: CHAF1A Antibody [NB100-74609] - Human lung cancer. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunohistochemistry-Paraffin-NB100-74609-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CHAF1A Antibody [NB100-74609] - Detection of Human Caf1p150 on HeLa whole cell lysate using NB100-74609. Caf1p150 was also immunoprecipitated by rabbit anti-Caf1p150 antibodies NB100-74608 and NB100-74610.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Western-Blot-NB100-74609-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHAF1A Antibody [NB100-74609] - Detection of human Caf1p150 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Caf1p150 antibody NB100-74609 used for IP at 3 ug per reaction. Caf1p150 was also immunoprecipitated by another rabbit anti-Caf1p150 antibody. For blotting immunoprecipitated Caf1p150, NB100-74609 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-Immunoprecipitation-NB100-74609-img0004.jpg)
![Western Blot: ALDH7A1 Antibody [NBP2-15342] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ALDH7A1-Antibody-Western-Blot-NBP2-15342-img0002.jpg "Western Blot: ALDH7A1 Antibody [NBP2-15342] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ALDH7A1 Antibody [NBP2-15342] - Paraffin-embedded mouse heart. ALDH7A1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ALDH7A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15342-img0004.jpg)
![Immunohistochemistry-Paraffin: ALDH7A1 Antibody [NBP2-15342] - Paraffin-embedded Mahlarvu xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH7A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15342-img0001.jpg)
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0002.jpg "Western Blot: SMC4 Antibody [NB100-56394] - analysis of CAP-C in Ramos cell lysate using this antibody.")
![Flow Cytometry: SMC4 Antibody [NB100-56394] - Western blot analysis of CAP-C in Ramos cell lysate using NB100-56394 at 2 ug/ml.](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NB100-56394-img0001.jpg)