| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93808-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97472-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06239-50ul | |||
![Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] - Analysis of NIH-3T3 cells using SQLE . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SQLE-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93808-img0002.jpg "Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] - Analysis of NIH-3T3 cells using SQLE . Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] - Analysis of HeLa cells using SQLE . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SQLE-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93808-img0001.jpg)
![Western Blot: SQLE Antibody [NBP2-93808] - Analysis of HepG2, using SQLE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp2-93808_rabbit-polyclonal-sqle-antibody-western-blot-146202312413..jpg)
![Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] -Analysis of HepG2 cells using SQLE Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93808_rabbit-polyclonal-sqle-antibody-immunocytochemistry-immunofluorescence-146202312556..jpg)
![Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] -Analysis of PC-12 cells using SQLE Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93808_rabbit-polyclonal-sqle-antibody-immunocytochemistry-immunofluorescence-146202312726..jpg)
![Western Blot: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Western blot analysis of extracts of various cell lines, using RUVBL1 Rabbit mAb (NBP3-16583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-7Q2U8-Western-Blot-NBP3-16583-img0002.jpg "Western Blot: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Western blot analysis of extracts of various cell lines, using RUVBL1 Rabbit mAb (NBP3-16583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Immunohistochemistry of paraffin-embedded mouse testis using RUVBL1 Rabbit mAb (NBP3-16583) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-7Q2U8-Immunohistochemistry-Paraffin-NBP3-16583-img0001.jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [NBP3-16583] -Analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-chromatin-immunoprecipitation-78202411528..jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-522025126537.jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [RUVBL1] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-52202517162323.jpg)
![Western Blot: KIF22 Antibody (1E3) [NBP2-37550] - Analysis using KID mAb against HEK293 (1) and KID(AA: 225-419)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/KIF22-Antibody-1E3-Western-Blot-NBP2-37550-img0003.jpg "Western Blot: KIF22 Antibody (1E3) [NBP2-37550] - Analysis using KID mAb against HEK293 (1) and KID(AA: 225-419)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: KIF22 Antibody (1E3) [NBP2-37550] - Analysis of colon cancer tissues using KID mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/KIF22-Antibody-1E3-Immunohistochemistry-NBP2-37550-img0006.jpg)
![Flow Cytometry: KIF22 Antibody (1E3) [NBP2-37550] - Analysis of NIH/3T3 cells using KID mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/KIF22-Antibody-1E3-Flow-Cytometry-NBP2-37550-img0004.jpg)
![Western Blot: KIF22 Antibody (1E3) [NBP2-37550] - Aalysis using KID mAb against human KID (AA: 225-419) recombinant protein. (Expected MW is 47 kDa)](http://images.novusbio.com/fullsize/KIF22-Antibody-1E3-Western-Blot-NBP2-37550-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF22 Antibody (1E3) [NBP2-37550] - Analysis of rectum cancer tissues using KID mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/KIF22-Antibody-1E3-Immunohistochemistry-NBP2-37550-img0005.jpg)
![ELISA: KIF22 Antibody (1E3) [NBP2-37550] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/KIF22-Antibody-1E3-ELISA-NBP2-37550-img0001.jpg)
![Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Western-Blot-NBP2-37596-img0003.jpg "Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of U251 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Immunocytochemistry-Immunofluorescence-NBP2-37596-img0004.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Immunohistochemistry-Paraffin-NBP2-37596-img0009.jpg)
![Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Western-Blot-NBP2-37596-img0002.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Immunohistochemistry-Paraffin-NBP2-37596-img0008.jpg)
![ELISA: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-ELISA-NBP2-37596-img0001.jpg)
![Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Western-Blot-NBP2-70383-img0001.jpg "Western Blot: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC6.")
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0006.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0002.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0003.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0004.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0005.jpg)
![Immunohistochemistry: Cdc6 Antibody (OTI1A4) - Azide and BSA Free [NBP2-70383] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Cdc6-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70383-img0007.jpg)
![Western Blot: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3.](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Western-Blot-NBP2-71338-img0001.jpg "Western Blot: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP3.")
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0006.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0002.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0003.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0004.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0005.jpg)
![Immunohistochemistry: DAP3 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71338] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DAP3-Antibody-OTI1B12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71338-img0007.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)
![Western Blot: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/50,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 5: Jurkat cell lysate Lane 6: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: PC-12 cell lysate Lane 10: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 21 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32763) at 1/50,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-288202412461610.jpg "Western Blot: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/50,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 5: Jurkat cell lysate Lane 6: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: PC-12 cell lysate Lane 10: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 21 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32763) at 1/50,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32763) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29820241411549.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling eIF4B with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/20,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/20,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29202410484885.jpg)
![Flow Cytometry: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Flow cytometric analysis of Jurkat cells labeling eIF4B. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32763, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29202416453295.jpg)
![Western Blot: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Western blot analysis of integrin beta 4 binding protein on different lysates with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32303) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-27820241631643.jpg "Western Blot: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Western blot analysis of integrin beta 4 binding protein on different lysates with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32303) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32303) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-29820241435273.jpg)
![Immunocytochemistry/ Immunofluorescence: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Immunocytochemistry analysis of HepG2 cells labeling integrin beta 4 binding protein with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32303) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-29202410234218.jpg)
![Flow Cytometry: integrin beta 4 binding protein Antibody (A6A3-R) [NBP3-32303] - Flow cytometric analysis of HepG2 cells labeling integrin beta 4 binding protein. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32303, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32303_mouse-integrin-beta-4-binding-protein-mab-a6a3-r-29202416453222.jpg)
![Western Blot: MRPL12 Antibody [NBP2-97472] - Anti-MRPL12 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/MRPL12-Antibody-Western-Blot-NBP2-97472-img0006.jpg "Western Blot: MRPL12 Antibody [NBP2-97472] - Anti-MRPL12 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa")
![Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-97472] - Immunochemical staining of human MRPL12 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunohistochemistry-Paraffin-NBP2-97472-img0005.jpg)
![Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-97472] - Immunochemical staining of human MRPL12 in human colon with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunohistochemistry-Paraffin-NBP2-97472-img0004.jpg)
![Immunoprecipitation: MRPL12 Antibody [NBP2-97472] - MRPL12 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 ul anti-MRPL12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPL12 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/MRPL12-Antibody-Immunoprecipitation-NBP2-97472-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MRPL12 Antibody [NBP2-97472] - Immunofluorescence staining of MRPL12 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97472 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97472_rabbit-polyclonal-mrpl12-antibody-2352025166477.png)
![Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa](http://images.novusbio.com/fullsize/CLPP-Antibody-Western-Blot-NBP3-06239-img0003.jpg "Western Blot: CLPP Antibody [NBP3-06239] - Anti-CLPP rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: K562. Whole Cell LysateLane D: A549 Whole Cell LysateLysatesroteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:26 kDa")
![Immunoprecipitation: CLPP Antibody [NBP3-06239] -](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunoprecipitation-NBP3-06239-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human liver with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251433153.png)
![Immunohistochemistry-Paraffin: CLPP Antibody - BSA Free [NBP3-06239] - Immunochemical staining of human CLPP in human pancreas with NBP3-06239 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-2352025112932.png)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP3-06239] - Immunofluorescence staining of CLPP in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06239 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-06239_rabbit-polyclonal-clpp-antibody-23520251633504.png)