| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18578-25ul | |||
| Azide and BSA Free | NBP3-44376 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84876-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86908-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18566-25ul | |||
| Azide and BSA Free | NBP3-44375 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03484-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52436-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37488-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56499SS | |||
![Western Blot: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human cell line U-251 MG and human cell line MCF-7.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Western-Blot-NBP3-18578-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human cell line U-251 MG and human cell line MCF-7.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0008.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human skin and skeletal muscle tissues using NBP3-18578 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0001.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human cervical cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0002.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human breast cancer (metastasis to ovary) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0006.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0007.jpg)
![Immunohistochemistry-Paraffin: EIF3A Antibody [NBP1-84876] - Staining of human cerebral cortex, colon, kidney and testis using Anti-EIF3A antibody NBP1-84876 (A) shows similar protein distribution across tissues to independent antibody NBP1-84875 (B).](http://images.novusbio.com/fullsize/EIF3A-Antibody-Immunohistochemistry-Paraffin-NBP1-84876-img0006.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EIF3A Antibody [NBP1-84876] - Staining of human cerebral cortex, colon, kidney and testis using Anti-EIF3A antibody NBP1-84876 (A) shows similar protein distribution across tissues to independent antibody NBP1-84875 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: EIF3A Antibody [NBP1-84876] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/EIF3A-Antibody-Western-Blot-NBP1-84876-img0005.jpg)
![Immunohistochemistry-Paraffin: EIF3A Antibody [NBP1-84876] - Staining of human kidney.](http://images.novusbio.com/fullsize/EIF3A-Antibody-Immunohistochemistry-Paraffin-NBP1-84876-img0009.jpg)
![Western Blot: EIF3A Antibody [NBP1-84876] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/EIF3A-Antibody-Western-Blot-NBP1-84876-img0004.jpg)
![Immunohistochemistry-Paraffin: EIF3A Antibody [NBP1-84876] - Staining of human testis.](http://images.novusbio.com/fullsize/EIF3A-Antibody-Immunohistochemistry-Paraffin-NBP1-84876-img0007.jpg)
![Immunohistochemistry-Paraffin: EIF3A Antibody [NBP1-84876] - Staining of human colon.](http://images.novusbio.com/fullsize/EIF3A-Antibody-Immunohistochemistry-Paraffin-NBP1-84876-img0008.jpg)
![Immunohistochemistry-Paraffin: EIF3A Antibody [NBP1-84876] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/EIF3A-Antibody-Immunohistochemistry-Paraffin-NBP1-84876-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Treacher Collins syndrome protein Antibody [NBP1-86908] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Treacher-Collins-syndrome-protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86908-img0007.jpg "Immunocytochemistry/Immunofluorescence: Treacher Collins syndrome protein Antibody [NBP1-86908] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Treacher Collins syndrome protein Antibody [NBP1-86908] - Staining of human testis shows weak to moderate nuclear and nucleolar positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Treacher-Collins-syndrome-protein-Antibody-Immunohistochemistry-Paraffin-NBP1-86908-img0016.jpg)
![Immunohistochemistry-Paraffin: Treacher Collins syndrome protein Antibody [NBP1-86908] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Treacher-Collins-syndrome-protein-Antibody-Immunohistochemistry-Paraffin-NBP1-86908-img0013.jpg)
![Immunohistochemistry-Paraffin: Treacher Collins syndrome protein Antibody [NBP1-86908] - Staining of human liver shows very low positivity in hepatocytes.](http://images.novusbio.com/fullsize/Treacher-Collins-syndrome-protein-Antibody-Immunohistochemistry-Paraffin-NBP1-86908-img0014.jpg)
![Immunohistochemistry-Paraffin: Treacher Collins syndrome protein Antibody [NBP1-86908] - Staining of human rectum shows moderate nuclear and nucleolar positivity in glandular cells.](http://images.novusbio.com/fullsize/Treacher-Collins-syndrome-protein-Antibody-Immunohistochemistry-Paraffin-NBP1-86908-img0015.jpg)
![Western Blot: CD44 Antibody (CL13324) [NBP3-18566] - Analysis in human cell line U-251 MG and human cell line MCF-7.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Western-Blot-NBP3-18566-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody (CL13324) [NBP3-18566] - Analysis in human cell line U-251 MG and human cell line MCF-7.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0008.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Analysis in human cervix, uterine and skeletal muscle tissues using NBP3-18566 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0001.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0002.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human cervical cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human ovarian cancer (low grade carcinoma) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human uterine cervix shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human lymph node shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0006.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0007.jpg)
![Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Western-Blot-NB100-74526-img0005.jpg "Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human kidney tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0010.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human tonsil tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0014.jpg)
![Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts of various cell lines, using Enolase 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP3-03484-img0006.jpg "Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts of various cell lines, using Enolase 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of U-2 OS cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03484-img0002.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Analysis of 200ug extracts of HeLa cells using Enolase 1 antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03484-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of NIH-3T3 cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03484-img0001.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Paraffin-embedded human colon carcinoma using Enolase 1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03484-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using Enolase 1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Knockout-Validated-NBP3-03484-img0005.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0007.jpg "Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.")
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0003.jpg)
![Flow Cytometry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Flow-Cytometry-NBP2-52436-img0001.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0005.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0004.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0002.jpg)
![ELISA: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-ELISA-NBP2-52436-img0006.jpg)
![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0003.jpg "Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody (1F5) [NBP2-37488] - Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunocytochemistry-Immunofluorescence-NBP2-37488-img0004.jpg)
![Immunohistochemistry: eIF4B Antibody (1F5) [NBP2-37488] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunohistochemistry-NBP2-37488-img0006.jpg)
![Flow Cytometry: eIF4B Antibody (1F5) [NBP2-37488] - Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Flow-Cytometry-NBP2-37488-img0005.jpg)
![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0002.jpg)
![ELISA: eIF4B Antibody (1F5) [NBP2-37488] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-ELISA-NBP2-37488-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)
![Western Blot: YY1 Antibody [NBP1-46218] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-YY1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. YY1 was also immunoprecipitated by rabbit anti-YY1 antibody NBP1-46217, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/YY1-Antibody-Western-Blot-NBP1-46218-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: YY1 Antibody [NBP1-46218] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-YY1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. YY1 was also immunoprecipitated by rabbit anti-YY1 antibody NBP1-46217, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: YY1 Antibody [NBP1-46218] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-YY1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/YY1-Antibody-Immunohistochemistry-NBP1-46218-img0011.jpg)
![Western Blot: Importin-9 Antibody [NB100-56499] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human Importin-9 Polyclonal Antibody (Catalog # NB100-56499) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Importin-9 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Importin-9-Antibody-Knockout-Validated-NB100-56499-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Importin-9 Antibody [NB100-56499] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Importin-9 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human Importin-9 Polyclonal Antibody (Catalog # NB100-56499) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Importin-9 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Importin-9 Antibody [NB100-56499] - Western blot analysis of Importin-9 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using NB100-56499 at 1 ug/ml.](http://images.novusbio.com/fullsize/Importin-9-Antibody-Western-Blot-NB100-56499-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16838-img0002.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.")
![Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-16838] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-HIST1H3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16838-img0009.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 ug of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16838-img0003.jpg)
![Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (NBP2-16838) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16838_rabbit-polyclonal-hist1h3a-antibody-immunocytochemistry-immunofluorescence-2611202518261313.jpg)
