| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35411-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59445-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03161-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2646-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50537-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11346-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98757-50ul | |||
![Western Blot: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Western blot analysis of various lysates using Proteasome 20S alpha 5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35411_rabbit-proteasome-20s-alpha-5-pab-1012202411334047.jpg "Western Blot: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Western blot analysis of various lysates using Proteasome 20S alpha 5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunoprecipitation: Proteasome 20S alpha 5 Antibody [NBP3-35411] - Immunoprecipitation analysis of 600 ug extracts of Mouse liver cells using 3 ug Proteasome 20S alpha 5 antibody. Western blot was performed from the immunoprecipitate using Proteasome 20S alpha 5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35411_rabbit-proteasome-20s-alpha-5-pab-1012202411404039.jpg)
![Western Blot: Podoplanin Antibody (56F7) [NBP2-59445] - Analysis in HeLa cell lysates (40ug).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-56F7-Western-Blot-NBP2-59445-img0001.jpg "Western Blot: Podoplanin Antibody (56F7) [NBP2-59445] - Analysis in HeLa cell lysates (40ug).")
![Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (56F7) [NBP2-59445] - Analysis of PDPN in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDPN antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-56F7-Immunocytochemistry-Immunofluorescence-NBP2-59445-img0002.jpg)
![Western Blot: Peroxiredoxin 3 Antibody - Azide and BSA Free [Peroxiredoxin 3] - Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1min.](http://images.novusbio.com/fullsize/nbp3-03161_rabbit-polyclonal-peroxiredoxin-3-antibody-5220251639631.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 3 Antibody - Azide and BSA Free [Peroxiredoxin 3] - Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1min.")
![Western Blot: Cyclin D1 Antibody (OTI1G2) - Azide and BSA Free [NBP2-70244] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cyclin D1.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-OTI1G2-Azide-and-BSA-Free-Western-Blot-NBP2-70244-img0001.jpg "Western Blot: Cyclin D1 Antibody (OTI1G2) - Azide and BSA Free [NBP2-70244] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cyclin D1.")
![Western Blot: Cyclin D1 Antibody (OTI1G2) - Azide and BSA Free [NBP2-70244] - Analysis of extracts (10ug) from 2 different cell lines by using anti-CCND1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-OTI1G2-Azide-and-BSA-Free-Western-Blot-NBP2-70244-img0003.jpg)
![Western Blot: KRAS Antibody (OTI1D1) - Azide and BSA Free [NBP2-71078] - Total cell lysates from human cancer cell lines PC-3, HeLa and HT-29 cells were analyzed by Western blot. PVDF membrane was probed with Human KRAS (OTI1D1) Antibody (NBP2-45535). A specific band was detected for KRAS at approximately 21 kDa. Image from ve](http://images.novusbio.com/fullsize/KRAS-Antibody-OTI1D1-Azide-and-BSA-Free-Western-Blot-NBP2-71078-img0002.jpg "Western Blot: KRAS Antibody (OTI1D1) - Azide and BSA Free [NBP2-71078] - Total cell lysates from human cancer cell lines PC-3, HeLa and HT-29 cells were analyzed by Western blot. PVDF membrane was probed with Human KRAS (OTI1D1) Antibody (NBP2-45535). A specific band was detected for KRAS at approximately 21 kDa. Image from ve")
![Western Blot: KRAS Antibody (OTI1D1) - Azide and BSA Free [NBP2-71078] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS.](http://images.novusbio.com/fullsize/KRAS-Antibody-OTI1D1-Azide-and-BSA-Free-Western-Blot-NBP2-71078-img0001.jpg)
![Western Blot: Catenin alpha 1 Antibody [NB120-11346] - Rat embryonic brain (S1) extract was separated on SDS-PAGE and probed with IGG FRACTION AB TO alpha-E-CATENIN (RB). The antibody was developed with HRP Anti-Rabbit IgG and an ECL substrate. Antibody dilution 1:2000.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Western-Blot-NB120-11346-img0002.jpg "Western Blot: Catenin alpha 1 Antibody [NB120-11346] - Rat embryonic brain (S1) extract was separated on SDS-PAGE and probed with IGG FRACTION AB TO alpha-E-CATENIN (RB). The antibody was developed with HRP Anti-Rabbit IgG and an ECL substrate. Antibody dilution 1:2000.")
![Immunocytochemistry/Immunofluorescence: Catenin alpha 1 Antibody [NB120-11346] - MDCK Madin Darby Canine Kidney cells were fixed and permeabilized with methanol. Fixed cells were stained with 1:100 Anti-alpha-E-Catenin antibody produced in Rabbit The antibody was developed using 1:500 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11346-img0003.jpg)
![Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: 293T cell lysate, 20 ug/Lane Lane 3: HepG2 cell lysate, 20 ug/Lane Lane 4: Jurkat cell lysate, 20 ug/Lane Predicted band size: 183 kDa Observed band size: 183 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32274_mouse-dnmt1-mab-a3a8-r-278202416285256.jpg "Western Blot: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 ug/Lane Lane 2: 293T cell lysate, 20 ug/Lane Lane 3: HepG2 cell lysate, 20 ug/Lane Lane 4: Jurkat cell lysate, 20 ug/Lane Predicted band size: 183 kDa Observed band size: 183 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32274) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (A3A8-R) [NBP3-32274] - Immunocytochemistry analysis of HeLa cells labeling DNMT1 with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DNMT1 antibody (NBP3-32274) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32274_mouse-dnmt1-mab-a3a8-r-2920241192358.jpg)
![Western Blot: CBX3 Antibody [NBP2-98757] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-98757-img0002.jpg "Western Blot: CBX3 Antibody [NBP2-98757] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-98757] - Immunofluorescence staining of CBX3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98757 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to .](http://images.novusbio.com/fullsize/nbp2-98757_rabbit-polyclonal-cbx3-antibody-235202514371336.png)
![Western Blot: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32174) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32174_rabbit-cdc6-p-ser54-mab-je59-52-278202416285220.jpg "Western Blot: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32174) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cdc6 [p Ser54] Antibody (JE59-52) [NBP3-32174] - Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Cdc6 antibody (NBP3-32174) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32174) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32174_rabbit-cdc6-p-ser54-mab-je59-52-29820241420921.jpg)
![Western Blot: CRABP2 Antibody (JE65-66) [NBP3-32214] - Western blot analysis of CRABP2 on MCF-7 cell lysates with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 16 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32214) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32214_rabbit-crabp2-mab-je65-66-278202415242666.jpg "Western Blot: CRABP2 Antibody (JE65-66) [NBP3-32214] - Western blot analysis of CRABP2 on MCF-7 cell lysates with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 16 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32214) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CRABP2 Antibody (JE65-66) [NBP3-32214] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CRABP2 antibody (NBP3-32214) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32214) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32214_rabbit-crabp2-mab-je65-66-298202414161824.jpg)