| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56096SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33447-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73472 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37414-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80687 | |||
![Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Immunohistochemistry-Paraffin-NBP2-03952-img0016.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Podoplanin (NZ-1.2) was detected in immersion fixed paraffin-embedded sections of human appendix using Rat Anti-Human Podoplanin Monoclonal Antibody (Catalog # NBP2-03952) at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface.")
![Western Blot: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Detection of lysate from human A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Western-Blot-NBP2-03952-img0014.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - A surface stain was performed on Ntera2 cells with Podoplanin Antibody (NZ-1.2) NBP2-03952AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0017.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0009.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.15 ug) of Podoplanin antibody (red) and 0.15 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0010.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the Azide Free version of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 0.2 ug of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green). Flow kit and anti-rat PE secondary were used for this test.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0012.jpg)
![Flow Cytometry: Podoplanin Antibody (NZ-1.2) [NBP2-03952] - Analysis using the PE conjugate of NBP2-03952. Staining using Podoplanin antibody. Human NCCIT cells were probed using 10 ul (0.2 ug) of Podoplanin antibody (red) and 0.2 ug of isotype control antibody (green).](http://images.novusbio.com/fullsize/Podoplanin-Antibody-NZ-1-2-Flow-Cytometry-NBP2-03952-img0013.jpg)
![Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56096-img0003.jpg "Western Blot: Bax Antibody [NB100-56096] - Analysis of Bax in mouse using this antibody. 1. In vitro translated mouse Bax. 2. Normal cortex. 3. Ischemic hippocampus. 4. Ischemic hippocampus. Ishemic samples were prepared 6 hr post middle cerebral artery occlusion. The results show the Bax expression increased following ischemia.")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56096] - Mouse tissue sections stained for Bax using NB100-56096 at 1:2000. A. Pancreas. B. Brain showing expression of Bax in the Purkinge cells.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56096-img0002.jpg)
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0012.jpg "Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunocytochemistry-Immunofluorescence-H00006241-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunohistochemistry-Paraffin-H00006241-M01-img0009.jpg)
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0002.jpg)
![Immunoprecipitation: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of RRM2 transfected lysate using anti-RRM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RRM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunoprecipitation-H00006241-M01-img0010.jpg)
![ELISA: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 Antibody (1E1) [H00006241-M01] - Detection limit for recombinant GST tagged RRM2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-ELISA-H00006241-M01-img0013.jpg)
![Western Blot: SNRPA1 Antibody [NBP2-33447] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Western-Blot-NBP2-33447-img0005.jpg "Western Blot: SNRPA1 Antibody [NBP2-33447] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: SNRPA1 Antibody [NBP2-33447] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33447-img0006.jpg)
![Immunohistochemistry-Paraffin: SNRPA1 Antibody [NBP2-33447] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-33447-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SNRPA1 Antibody [NBP2-33447] - After indicated time periods of SNRPA1 knockdown, cells were collected and analyzed by western blotting. Forty-eight hours after SNRPA1 depletion, protein expression levels of RAD51 and Chk1 were already reduced. At 24h after siSNRPA1 transfection, gamma-H2AX levels transiently increased and then declined. Image collected and cropped by CiteAb from the following publication (nature.com/articles/oncsis201670), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Knockdown-Validated-NBP2-33447-img0008.jpg)
![Immunoprecipitation: SNRPA1 Antibody [NBP2-33447] - Immunoprecipitation of SNRPA1 using NBP2-33447 or Rabbit IgG isotype control at a 1:100 dilution, followed by pulldown with Protein A magnetic beads. IP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunoprecipitation-NBP2-33447-img0009.jpg)
![Western Blot: SNRPA1 Antibody [NBP2-33447] - Recruitment kinetics of SNRPA1 & SF3A3 after laser microirradiation & SNRPA1-dependent recruitment of RAD51 & BRCA1 to laser tracks. (a) Image-based cytometry reveals increased gamma H2AX formation after 72 h SNRPA1 knockdown in U2OS cells. SNRPA1 knockdown effciency is shown in the bar graph. U2OS cells transiently (b) or stably (c) expressing SNRPA1-GFP were microirradiated & protein recruitment followed in real-time. For transcription & splicing inhibition cells were pretreated with DRB (50 μM) or SSA (100 nM) for at least 1 h before damage induction. Representative confocal images & recruitment kinetics are shown. (d) As in (b & c) but in cells stably expressing SF3A3-GFP. (e) Generation of R-loops at laser tracks visualized by HB-GFP recruitment. HB-GFP recruitment kinetics resemble SNRPA1-GFP. (f) Schematic of SNRPA1 deletion constructs. (g) Representative confocal images of recruitment of wild-type & mutated GFP-tagged SNRPA1 after laser microirradiation. (h) Quantification of recruitment, non-recruitment & displacement of GFP-tagged SNRPA1 fusions. Ten cells were analyzed per condition. (i) Confirmation of siRNA-mediated depletion of SNRPA1 after 48 & 72 h knockdown in cells used in (j). (j) Recruitment of RAD51 & BRCA1 in control & SNRPA1-depleted U2OS cells. U2OS cells were transfected with control or SNRPA1 siRNA & 48 or 72 h later microirradiated, fixed after indicated repair times & stained for gamma H2AX, RAD51 & BRCA1. Representative confocal images & percentage of cells displaying RAD51 & BRCA1 recruitment are shown. Scale bar 5 or 10 μM (i). Statistically significant differences were determined using Student's t-test, *P12 foci), gamma H2AX (>12 foci), BRCA1 (>12 foci) & RPA (>12 foci) positive cells. For each condition, more than 400 cells were analyzed. (c) After indicated time periods of SNRPA1 knockdown, cells were collected & analyzed by western blotting. Forty-eight hours after SNRPA1 depletion, protein expression levels of RAD51 & Chk1 were already reduced. At 24 h after siSNRPA1 transfection, gamma H2AX levels transiently increased & then declined. The error bars represent s.e.m. from three independent experiments (n=3). Statistically significant differences between cells treated with control or splicing factor siRNA were determined using Student's t-test, *P<0.05, **P<0.01. Scale bar is 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27991914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33447_rabbit-polyclonal-snrpa1-antibody-31020241611355.jpg)
![Western Blot: hnRNP K Antibody [NBP1-30975] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP1-30975-img0011.jpg "Western Blot: hnRNP K Antibody [NBP1-30975] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: hnRNP K Antibody [NBP1-30975] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP K stained by hnRNP K antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30975-img0009.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0003.jpg)
![Western Blot: hnRNP K Antibody [NBP1-30975] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP1-30975-img0004.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded mouse urinary bladder. hnRNP K antibody dilution: 1:500.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0006.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded mouse colon. hnRNP K antibody dilution: 1:500.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0007.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded rat brain stem. hnRNP K antibody dilution: 1:500.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0008.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-910202420104460.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-91020242014677.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-910202420131260.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-91020242011484.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0008.jpg "Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0002.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0003.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human cervical carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0007.jpg)
![Simple Western: Cytochrome c Antibody [NBP2-21569] - Cytochrome C Antibodies (NBP2-21569), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 10 and 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-simple-western-204202311476.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063356.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063396.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-1610202419405762.jpg)
![Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Western-Blot-NBP1-48291-img0016.jpg "Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of")
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0018.jpg)
![Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody , and then analyzed by flow cytometry (1:100).](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Flow-Cytometry-NBP1-48291-img0017.jpg)
![Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Western-Blot-NBP1-48291-img0001.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0003.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0004.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0009.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0011.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0014.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0015.jpg)
![Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-Western-Blot-NBP2-37414-img0004.jpg "Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.")
![Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of cervical cancer tissues using PCNA antibody. Antibody dilution 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-Immunohistochemistry-Paraffin-NBP2-37414-img0008.jpg)
![Flow Cytometry: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-Flow-Cytometry-NBP2-37414-img0005.jpg)
![Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mAb against human PCNA recombinant protein. (Expected MW is 41.2 kDa)](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-Western-Blot-NBP2-37414-img0002.jpg)
![Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-Western-Blot-NBP2-37414-img0003.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of cervical cancer tissues using PCNA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-Immunohistochemistry-NBP2-37414-img0006.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of colon cancer tissues using PCNA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-Immunohistochemistry-NBP2-37414-img0007.jpg)
![ELISA: PCNA Antibody (7H4F8) [NBP2-37414] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PCNA-Antibody-7H4F8-ELISA-NBP2-37414-img0001.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74638-img0003.jpg "Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74639, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunoprecipitation-NB100-74638-img0002.jpg)
![Immunohistochemistry: Astrin Antibody [NB100-74638] - SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against Astrin & SKA3 & CREST antiserum (to mark centromeres) & stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against GFP (for detecting YFP-Astrin) & SKA3 & CREST antiserum & co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74638_rabbit-polyclonal-astrin-antibody-31020241612667.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88345-img0017.jpg "Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining in human testis and kidney tissues . Corresponding NCAPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0016.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0014.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88345 (A) shows similar protein distribution across tissues to independent antibody NBP1-88346 (B).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human skin shows nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human tonsil shows nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0015.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Simple Western lane view shows a specific band for NCAPH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0006.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Electropherogram image(s) of corresponding Simple Western lane view. NCAPH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0007.jpg)
![Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 .](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-6H2-B4-Immunoprecipitation-NBP2-24872-img0004.jpg "Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Immunoprecitation/analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 .")
![Immunoprecipitation: Cytochrome c Antibody (6H2.B4) [NBP2-24872] - Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-6H2-B4-Immunoprecipitation-NBP2-24872-img0005.jpg)