| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86635-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33447-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29624SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73472 | |||
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0012.jpg "Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunocytochemistry-Immunofluorescence-H00006241-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunohistochemistry-Paraffin-H00006241-M01-img0009.jpg)
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0002.jpg)
![Immunoprecipitation: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of RRM2 transfected lysate using anti-RRM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RRM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunoprecipitation-H00006241-M01-img0010.jpg)
![ELISA: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 Antibody (1E1) [H00006241-M01] - Detection limit for recombinant GST tagged RRM2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-ELISA-H00006241-M01-img0013.jpg)
![Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NBP1-86635-img0007.jpg "Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86635-img0008.jpg)
![Immunohistochemistry-Paraffin: SMC4 Antibody [NBP1-86635] - Staining of human kidney shows strong cytoplasmic positivity with granular pattern in tubule cells.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunohistochemistry-Paraffin-NBP1-86635-img0002.jpg)
![Western Blot: SMC4 Antibody [NBP1-86635] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-544](http://images.novusbio.com/fullsize/SMC4-Antibody-Western-Blot-NBP1-86635-img0006.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86635_rabbit-polyclonal-smc4-antibody-30420251371429.jpg)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
![Western Blot: SNRPA1 Antibody [NBP2-33447] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Western-Blot-NBP2-33447-img0005.jpg "Western Blot: SNRPA1 Antibody [NBP2-33447] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: SNRPA1 Antibody [NBP2-33447] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33447-img0006.jpg)
![Immunohistochemistry-Paraffin: SNRPA1 Antibody [NBP2-33447] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-33447-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SNRPA1 Antibody [NBP2-33447] - After indicated time periods of SNRPA1 knockdown, cells were collected and analyzed by western blotting. Forty-eight hours after SNRPA1 depletion, protein expression levels of RAD51 and Chk1 were already reduced. At 24h after siSNRPA1 transfection, gamma-H2AX levels transiently increased and then declined. Image collected and cropped by CiteAb from the following publication (nature.com/articles/oncsis201670), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Knockdown-Validated-NBP2-33447-img0008.jpg)
![Immunoprecipitation: SNRPA1 Antibody [NBP2-33447] - Immunoprecipitation of SNRPA1 using NBP2-33447 or Rabbit IgG isotype control at a 1:100 dilution, followed by pulldown with Protein A magnetic beads. IP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunoprecipitation-NBP2-33447-img0009.jpg)
![Western Blot: SNRPA1 Antibody [NBP2-33447] - Recruitment kinetics of SNRPA1 & SF3A3 after laser microirradiation & SNRPA1-dependent recruitment of RAD51 & BRCA1 to laser tracks. (a) Image-based cytometry reveals increased gamma H2AX formation after 72 h SNRPA1 knockdown in U2OS cells. SNRPA1 knockdown effciency is shown in the bar graph. U2OS cells transiently (b) or stably (c) expressing SNRPA1-GFP were microirradiated & protein recruitment followed in real-time. For transcription & splicing inhibition cells were pretreated with DRB (50 μM) or SSA (100 nM) for at least 1 h before damage induction. Representative confocal images & recruitment kinetics are shown. (d) As in (b & c) but in cells stably expressing SF3A3-GFP. (e) Generation of R-loops at laser tracks visualized by HB-GFP recruitment. HB-GFP recruitment kinetics resemble SNRPA1-GFP. (f) Schematic of SNRPA1 deletion constructs. (g) Representative confocal images of recruitment of wild-type & mutated GFP-tagged SNRPA1 after laser microirradiation. (h) Quantification of recruitment, non-recruitment & displacement of GFP-tagged SNRPA1 fusions. Ten cells were analyzed per condition. (i) Confirmation of siRNA-mediated depletion of SNRPA1 after 48 & 72 h knockdown in cells used in (j). (j) Recruitment of RAD51 & BRCA1 in control & SNRPA1-depleted U2OS cells. U2OS cells were transfected with control or SNRPA1 siRNA & 48 or 72 h later microirradiated, fixed after indicated repair times & stained for gamma H2AX, RAD51 & BRCA1. Representative confocal images & percentage of cells displaying RAD51 & BRCA1 recruitment are shown. Scale bar 5 or 10 μM (i). Statistically significant differences were determined using Student's t-test, *P12 foci), gamma H2AX (>12 foci), BRCA1 (>12 foci) & RPA (>12 foci) positive cells. For each condition, more than 400 cells were analyzed. (c) After indicated time periods of SNRPA1 knockdown, cells were collected & analyzed by western blotting. Forty-eight hours after SNRPA1 depletion, protein expression levels of RAD51 & Chk1 were already reduced. At 24 h after siSNRPA1 transfection, gamma H2AX levels transiently increased & then declined. The error bars represent s.e.m. from three independent experiments (n=3). Statistically significant differences between cells treated with control or splicing factor siRNA were determined using Student's t-test, *P<0.05, **P<0.01. Scale bar is 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27991914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33447_rabbit-polyclonal-snrpa1-antibody-31020241611355.jpg)
![Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Western-Blot-NBP1-86928-img0016.jpg "Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining in human testis and kidney tissues using anti-TOP2A antibody. Corresponding TOP2A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Immunohistochemistry-Paraffin-NBP1-86928-img0014.jpg)
![Western Blot: TOP2A Antibody [NBP1-86928] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Western-Blot-NBP1-86928-img0015.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Immunohistochemistry-Paraffin-NBP1-86928-img0012.jpg)
![Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Immunohistochemistry-Paraffin-NBP1-86928-img0013.jpg)
![Western Blot: ErbB2/HER2 Antibody [NBP2-29624] - Western blot analysis of ErbB2/HER2 in SKOV3 cell lysate.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-Western-Blot-NBP2-29624-img0001.jpg "Western Blot: ErbB2/HER2 Antibody [NBP2-29624] - Western blot analysis of ErbB2/HER2 in SKOV3 cell lysate.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB3 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. The cells in the cores of some tumor areas were negative for ErbB2.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP2-29624-img0003.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB2 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP2-29624-img0002.jpg)
![Western Blot: Peroxiredoxin 1 Antibody [NBP1-82558] - Analysis in human cell lines PC-3 and SK-MEL-30 using Anti-PRDX1 antibody. Corresponding PRDX1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Western-Blot-NBP1-82558-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Peroxiredoxin 1 Antibody [NBP1-82558] - Analysis in human cell lines PC-3 and SK-MEL-30 using Anti-PRDX1 antibody. Corresponding PRDX1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82558-img0007.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0014.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0011.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0012.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody [NBP1-82558] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Peroxiredoxin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-82558-img0013.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human endometrial carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0008.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0002.jpg)
![Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytochrome-C-Antibody-Western-Blot-NBP2-21569-img0003.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Western-Blot-NBP2-21569-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human cervical carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-Immunohistochemistry-Paraffin-NBP2-21569-img0007.jpg)
![Simple Western: Cytochrome c Antibody [NBP2-21569] - Cytochrome C Antibodies (NBP2-21569), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 10 and 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-simple-western-204202311476.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063356.jpg)
![Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-91020242063396.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-21569_rabbit-polyclonal-cytochrome-c-antibody-1610202419405762.jpg)
![Western Blot: hnRNP K Antibody [NBP1-30975] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP1-30975-img0011.jpg "Western Blot: hnRNP K Antibody [NBP1-30975] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP K antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: hnRNP K Antibody [NBP1-30975] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP K stained by hnRNP K antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30975-img0009.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0003.jpg)
![Western Blot: hnRNP K Antibody [NBP1-30975] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Western-Blot-NBP1-30975-img0004.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded mouse urinary bladder. hnRNP K antibody dilution: 1:500.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0006.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded mouse colon. hnRNP K antibody dilution: 1:500.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0007.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded rat brain stem. hnRNP K antibody dilution: 1:500.](http://images.novusbio.com/fullsize/hnRNP-K-Antibody-Immunohistochemistry-Paraffin-NBP1-30975-img0008.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-910202420104460.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-91020242014677.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-910202420131260.jpg)
![Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30975_rabbit-polyclonal-hnrnp-k-antibody-91020242011484.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0025.jpg "Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0026.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse liver. PCNA antibody dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0019.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0020.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0014.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0013.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0016.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0017.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Rat hind brain. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0018.jpg)
![Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NBP1-32075-img0023.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32075_rabbit-polyclonal-pcna-antibody-910202420131282.jpg)
![Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0046.jpg "<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-NB100-456-img0044.jpg)
![Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.](http://images.novusbio.com/fullsize/PCNA-Antibody-Flow-Cytometry-NB100-456-img0004.jpg)
![Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0041.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NB100-456-img0043.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NB100-456-img0047.jpg)
![Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Western-Blot-NBP1-48291-img0016.jpg "Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of")
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0018.jpg)
![Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody , and then analyzed by flow cytometry (1:100).](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Flow-Cytometry-NBP1-48291-img0017.jpg)
![Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Western-Blot-NBP1-48291-img0001.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0003.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0004.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0009.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0011.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0014.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-OTI1D4-Immunohistochemistry-Paraffin-NBP1-48291-img0015.jpg)