| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89729-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92502-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79854 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92947-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52501-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Fumarase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89814-img0015.jpg "Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-89814] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.")
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse hippocampus shows neuronal immunoreactivity in the CA3 layer.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342022.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse globus pallidus shows immunoreactivity in neurons.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133407.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse medulla shows positivity in the neurons of the facial nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342036.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of mouse olfactory bulb shows neuronal positivity in the anterior olfactory nucleus.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334012.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342060.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-17520231334201.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-1752023133402.jpg)
![Immunohistochemistry: Fumarase Antibody [NBP1-89814] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp1-89814_rabbit-polyclonal-fumarase-antibody-175202313342070.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Fumarase Antibody [NBP1-89814] - Analysis using Anti-FH antibody NBP1-89814 (A) shows similar pattern to independent antibody NBP1-89813 (B).](http://images.novusbio.com/fullsize/Fumarase-Antibody-Western-Blot-NBP1-89814-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89729-img0014.jpg "Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human cerebral cortex shows strong positivity in mitochondria in neurons.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-89729-img0020.jpg)
![Western Blot: THOC3 Antibody [NBP1-92502] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/THOC3-Antibody-Western-Blot-NBP1-92502-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: THOC3 Antibody [NBP1-92502] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: THOC3 Antibody [NBP1-92502] - Staining of human colon shows strong granular cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/THOC3-Antibody-Immunohistochemistry-Paraffin-NBP1-92502-img0006.jpg)
![Western Blot: THOC3 Antibody [NBP1-92502] - Analysis in human cell line PC-3.](http://images.novusbio.com/fullsize/THOC3-Antibody-Western-Blot-NBP1-92502-img0007.jpg)
![Western Blot: THOC3 Antibody [NBP1-92502] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/THOC3-Antibody-Western-Blot-NBP1-92502-img0009.jpg)
![Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Western-Blot-NBP2-79720-img0010.jpg "Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).")
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of paraformaldehyde-Raji cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0003.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0008.jpg)
![Flow Cytometry: RPSA Antibody (RPSA/2699) [NBP2-79720] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Flow-Cytometry-NBP2-79720-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of HeLa cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0002.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0004.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0005.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0006.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Tonsil stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0007.jpg)
![Protein Array: RPSA Antibody (RPSA/2699) [NBP2-79720] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RPSA Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Protein-Array-NBP2-79720-img0009.jpg)
![Western Blot: CD44 Antibody [NBP2-92947] - Analysis of extracts of various cell lines, using CD44 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP2-92947-img0005.jpg "Western Blot: CD44 Antibody [NBP2-92947] - Analysis of extracts of various cell lines, using CD44 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of RAW264.7 cells using CD44 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP2-92947] - Human lung cancer using CD44 Rabbit pAb at dilution of 1:250 (40x lens).](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP2-92947-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of A431 cells using CD44 at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of HeLa cells using CD44 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of HeLa cells using CD44 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP2-92947] - Human esophageal cancer using CD44 Rabbit pAb at dilution of 1:250 (40x lens).](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP2-92947-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD44 Antibody [NBP2-92947] - Analysis of extracts of HeLa cells, using CD44 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CD44-Antibody-Knockout-Validated-NBP2-92947-img0006.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0012.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunofluorescence-NBP2-52501-img0011.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0009.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0010.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0002.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0003.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Western-Blot-NBP2-52501-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunocytochemistry-Immunofluorescence-NBP2-52501-img0006.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Immunohistochemistry-Paraffin-NBP2-52501-img0008.jpg)
![Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-Flow-Cytometry-NBP2-52501-img0007.jpg)
![ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-1E8B7-ELISA-NBP2-52501-img0004.jpg)
![Western Blot: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERp72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERp72.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Western-Blot-NBP2-71488-img0002.jpg "Western Blot: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERp72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERp72.")
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human pancreas tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0008.jpg)
![Western Blot: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERp72 monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Western-Blot-NBP2-71488-img0001.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0003.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0004.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0005.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0006.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human lymphoma tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0007.jpg)
![Immunohistochemistry: ERp72 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71488] - Staining of paraffin-embedded Human tonsil using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71488-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845] - Hs68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Fibroblast Surface Protein antibody, Mouse monoclonal, Clone: 1B10. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Fibroblast-Surface-Protein-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NB100-1845-img0001.jpg "Immunocytochemistry/Immunofluorescence: Fibroblast Surface Protein Antibody (1B10) [NB100-1845] - Hs68 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:500 Anti-Fibroblast Surface Protein antibody, Mouse monoclonal, Clone: 1B10. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Western-Blot-NB110-38867-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-NB110-38867-img0005.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:500.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NB110-38867-img0001.jpg)
![Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:100.](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-Paraffin-NB110-38867-img0002.jpg)
![Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PP2C-gamma-PPM1G-Antibody-Immunohistochemistry-NB110-38867-img0004.jpg)
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: hnRNP A1 Antibody [NBP1-32161] - Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody [NBP1-32161] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32161-img0002.jpg)
![Immunohistochemistry-Paraffin: hnRNP A1 Antibody [NBP1-32161] - hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. hnRNP A1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Immunohistochemistry-Paraffin-NBP1-32161-img0008.jpg)
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0004.jpg)
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - Sample(30 ug of whole cell lysate) A:H1299 B:HeLa S3 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0005.jpg)
![Western Blot: hnRNP A1 Antibody [NBP1-32161] - A. 30 ug PC-12 whole cell lysate/extract.](http://images.novusbio.com/fullsize/hnRNP-A1-Antibody-Western-Blot-NBP1-32161-img0007.jpg)
![Immunohistochemistry: hnRNP A1 Antibody [NBP1-32161] - hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. hnRNP A1 antibody (NBP1-32161) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-32161_rabbit-polyclonal-hnrnp-a1-antibody-9102024205646.jpg)
![Western Blot: PABP Antibody [NBP2-19670] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-19670-img0012.jpg "Western Blot: PABP Antibody [NBP2-19670] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Paraffin-embedded mouse middle brain. PABP antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-19670-img0005.jpg)
![Western Blot: PABP Antibody [NBP2-19670] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-19670-img0009.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-19670-img0002.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Paraffin-embedded mouse testis. PABP antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-19670-img0004.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Mouse testis.PABP stained by PABP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-19670_rabbit-polyclonal-pabp-antibody-immunohistochemistry-paraffin-2532024111751..jpg)
![Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.](http://images.novusbio.com/fullsize/eIF3K-Antibody-Western-Blot-NB100-93304-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-310202416284928.jpg)
![Western Blot: eIF3K Antibody [NB100-93304] - TRS functions similarly to eIF4G & acts as an eIF4F analog. a Pull-down assay of co-expressed TRS-Strep with eIF4A- or eIF4G-FLAG in 293 T cells. TRS-Strep was pulled down with Strep-Tactin beads, & co-precipitation of eIF4A or 4 G was determined by immunoblotting with anti-FLAG antibody. EV, empty vector. * indicates a nonspecific band. b Immunoassay of the co-expression of different combinations of plasmids in 293T cells. Myc-TRS was immunoprecipitated with anti-Myc antibody, & co-precipitation of other proteins was determined using tag-specific antibodies. c Immunoassay of co-expressed eIF4A-FLAG with GST-fused full-length TRS or its various domains in 293T cells. eIF4A-FLAG was immunoprecipitated with anti-FLAG antibody, & co-precipitated TRS proteins were determined by immunoblotting with anti-GST antibody. d Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293 T cells transfected with siRNAs against TRS, 4EHP, or a non-targeting control (siCont). Cap-bound proteins were eluted from beads & immunoblotted with the indicated antibodies. Sepharose beads were used as a negative control. e Pull-down assay of endogenous translation initiation factors with m7GTP-Sepharose beads in 293T cells transfected with siRNAs against eIF4G, eIF4E, or siCont, & their suppression effects on cap-binding of other components, were determined as in (d). The data are representative of at least three experiments, each with similar results Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30902983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-93304_rabbit-polyclonal-eif3k-antibody-31020241628498.jpg)