| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59625 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05379-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51596-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50537-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-223SS | |||
![Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB100-56623-img0002.jpg "Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.")
![Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0045.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0049.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Western-Blot-NB100-273-img0002.jpg)
![Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-NB100-273-img0048.jpg)
![Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Nbs1-Antibody-Western-Blot-NB100-60654-img0017.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Nbs1-Antibody-Immunohistochemistry-NB100-60654-img0027.jpg)
![Protein Array: p53 Antibody (PAb122) [NBP2-59624] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-PAb122-Protein-Array-NBP2-59624-img0001.jpg "Protein Array: p53 Antibody (PAb122) [NBP2-59624] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29")
![Western Blot: 53BP1 Antibody [NBP3-14667] - Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] NBP3-14667 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 10 second](http://images.novusbio.com/fullsize/53BP1-Antibody-Western-Blot-NBP3-14667-img0001.jpg "Western Blot: 53BP1 Antibody [NBP3-14667] - Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] NBP3-14667 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 10 second")
![Immunohistochemistry: 53BP1 Antibody [NBP3-14667] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-53BP1 recombinant monoclonalantibody used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunohistochemistry-NBP3-14667-img0004.jpg)
![Immunohistochemistry: 53BP1 Antibody [NBP3-14667] - Sample: FFPE section of colon carcinoma. Antibody: Rabbitanti-53BP1 recombinant monoclonal antibody used at 1:250. Secondary:HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunohistochemistry-NBP3-14667-img0003.jpg)
![Immunoprecipitation: 53BP1 Antibody [NBP3-14667] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Rabbitanti-53BP1 recombinant monoclonal antibodyused for IP at 20 ul perreaction. 53BP1 was also immunoprecipitated by rabbitanti-53BP1 antibody for blottingimmunoprecipitated 53BP1, was used at 1:1000.Chemiluminescence with an exposure time of 10 second.](http://images.novusbio.com/fullsize/53BP1-Antibody-Immunoprecipitation-NBP3-14667-img0002.jpg)
![Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human and mouse SMC1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Western-Blot-NBP2-76412-img0001.jpg "Western Blot: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human and mouse SMC1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE HCT 116 cells by ICC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunocytochemistry-NBP2-76412-img0004.jpg)
![Immunohistochemistry: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (NBP2-76412). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunohistochemistry-NBP2-76412-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SMC1 Antibody (BL-205-2G8) [NBP2-76412] - Detection of human SMC1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-SMC1 recombinant monoclonal {BL-205-2G8] (NBP2-76412) and rabbit anti-SMC1 (NB100-204).](http://images.novusbio.com/fullsize/SMC1-Antibody-BL-205-2G8-Immunoprecipitation-NBP2-76412-img0005.jpg)
![Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Western-Blot-NBP3-13705-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunocytochemistry-Immunofluorescence-NBP3-13705-img0003.jpg)
![Immunohistochemistry-Paraffin: ATM [p Ser1981] Antibody (HL1062) [NBP3-13705] - ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATM-p-Ser1981-Antibody-HL1062-Immunohistochemistry-Paraffin-NBP3-13705-img0002.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13705_rabbit-atm-p-ser-1981-mab-hl1062-western-blot-261120251845135.jpg)
![Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (NBP3-13705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13705_rabbit-atm-p-ser-1981-mab-hl1062-western-blot-261120251836813.jpg)
![Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Western-Blot-NBP3-05379-img0005.jpg "Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded mouse brain using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded rat heart using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0001.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded human placenta using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0002.jpg)
![Western Blot: Chk1 [p Ser280] Antibody - Azide and BSA Free [Chk1] - Western blot analysis of lysates from 293T cells, using Chk1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05379_rabbit-polyclonal-chk1-p-ser280-antibody-52202516293974.jpg)
![Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Western-Blot-NBP1-51596-img0007.jpg "Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of NIH3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunocytochemistry-Immunofluorescence-NBP1-51596-img0010.jpg)
![Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Immunohistochemistry-Paraffin-NBP1-51596-img0008.jpg)
![Flow Cytometry: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/SMC1-Antibody-5B6-Flow-Cytometry-NBP1-51596-img0009.jpg)
![Western Blot: HDAC10 Antibody [NB600-223] - WB analysis of HDAC10 in HeLa cells with NB600-223 at a dilution of 1:250.](http://images.novusbio.com/fullsize/HDAC10-Antibody-Western-Blot-NB600-223-img0001.jpg "Western Blot: HDAC10 Antibody [NB600-223] - WB analysis of HDAC10 in HeLa cells with NB600-223 at a dilution of 1:250.")
![Simple Western: HDAC10 Antibody [NB600-223] - 1:25 dilution against the indicated concentration of protein lysate (Human SVG-A). Image from verified customer review.](http://images.novusbio.com/fullsize/HDAC10-Antibody-Simple-Western-NB600-223-img0002.jpg)
![Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.](http://images.novusbio.com/fullsize/Rad17-Antibody-Western-Blot-NBP2-27106-img0002.jpg "Western Blot: Rad17 Antibody [NBP2-27106] - Western blot analysis of Rad17 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-27106 at 3 ug/ml.")