| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57700-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99012-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-22071SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75924SS | |||
| Azide and BSA Free | NBP2-80828 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52518-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61122-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54748-25ul | |||
![Western Blot: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Western blot analysis of extracts of various cell lines, using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Western-Blot-NBP3-15282-img0004.jpg "Western Blot: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Western blot analysis of extracts of various cell lines, using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Immunohistochemistry of paraffin-embedded mouse kidney using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Immunohistochemistry-Paraffin-NBP3-15282-img0002.jpg)
![Western Blot: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Western blot analysis of extracts of A-549 cells, using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Western-Blot-NBP3-15282-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Immunohistochemistry of paraffin-embedded rat kidney using LAMP-2/CD107b Rabbit mAb (NBP3-15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Immunohistochemistry-Paraffin-NBP3-15282-img0001.jpg)
![Immunoprecipitation: LAMP-2/CD107b Antibody (5N8J3) [NBP3-15282] - Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug LAMP-2/CD107b antibody (NBP3-15282). Western blot was performed from the immunoprecipitate using LAMP-2/CD107b antibody (NBP3-15282) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-5N8J3-Immunoprecipitation-NBP3-15282-img0005.jpg)
![Immunohistochemistry: LAMP-2/CD107b Antibody (5N8J3) [LAMP-2/CD107b] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using LAMP-2/CD107b Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15282_rabbit-lamp-2-cd107b-mab-5n8j3-52202517235736.jpg)
![Immunohistochemistry: LAMP-2/CD107b Antibody (5N8J3) [LAMP-2/CD107b] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using LAMP-2/CD107b Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15282_rabbit-lamp-2-cd107b-mab-5n8j3-52202517251982.jpg)
![Immunohistochemistry: LAMP-2/CD107b Antibody (5N8J3) [LAMP-2/CD107b] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using LAMP-2/CD107b Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15282_rabbit-lamp-2-cd107b-mab-5n8j3-52202517251957.jpg)
![Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-57700] - Staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57700-img0001.jpg "Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-57700] - Staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-57700] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-57700-img0003.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-57700] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-57700_rabbit-polyclonal-pax2-antibody-immunohistochemistry-paraffin-1142024122412..jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99012] - Immunochemical staining Pax2 in human renal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-99012-img0004.jpg "Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99012] - Immunochemical staining Pax2 in human renal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99012] - Immunochemical staining Pax2 in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-99012-img0003.jpg)
![Western Blot: Pax2 Antibody [NBP2-99012] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:44 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99012_rabbit-polyclonal-pax2-antibody-western-blot-2102023101258..jpg)
![Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-99012] - Immunofluorescence staining of Pax2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99012 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-99012_rabbit-polyclonal-pax2-antibody-235202521525068.png)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99012] - Immunochemical staining of Pax2 in human kidney with NBP2-99012 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp2-99012_rabbit-polyclonal-pax2-antibody-235202514371328.png)
![Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Western-Blot-NB120-22071-img0007.jpg "Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.")
![Immunohistochemistry-Paraffin: IkB-alpha Antibody [NB120-22071] - Analysis of human breast using IkB-alpha antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-22071-img0005.jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.<br><br><strong>Capture:</strong> Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)<br><strong>Detection:</strong> HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)<br>Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.<br><br><strong>Capture:</strong> Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)<br><strong>Detection:</strong> HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).<br>Sample: Paraffin-embedded mouse brain.<br>Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)
![Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Western-Blot-NBP2-75924-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: LC3A Antibody (2312D) [NBP2-75924] - This LC3A antibody was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody (Catalog # NBP2-75924) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Immunohistochemistry-Paraffin-NBP2-75924-img0004.jpg)
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0004.jpg "Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0003.jpg)
![Flow Cytometry: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Flow-Cytometry-NBP2-52518-img0001.jpg)
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0002.jpg)
![ELISA: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-ELISA-NBP2-52518-img0006.jpg)
![Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Western-Blot-NBP2-61122-img0004.jpg "Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61122-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Tissue: Colon Cancer. Species: Human. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody (NBP2-61122) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunohistochemistry-Paraffin-NBP2-61122-img0003.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Analysis in human cerebral cortex and pancreas tissues using Anti-GFAP antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-54748-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Analysis in human cerebral cortex and pancreas tissues using Anti-GFAP antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-54748-img0006.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody [NBP2-54748] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunohistochemistry-Paraffin-NBP2-54748-img0005.jpg)
![ELISA: Tau [p Thr217] Antibody [NBP3-13481] - Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (NBP3-13481) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr217]-Antibody-ELISA-NBP3-13481-img0002.jpg "ELISA: Tau [p Thr217] Antibody [NBP3-13481] - Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (NBP3-13481) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.")
![IHC-P-Tau [p Thr217] Antibody-Rat brain. Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp3-13481_rabbit-tau-p-thr-217-pab-immunohistochemistry-paraffin-89202384342..jpg)
![Tau (phospho Thr217) antibody detects Tau (phospho Thr217) protein at nucleus and cytoskeleton by immunofluorescent analysis.</br>Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:250.</br>Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13481_rabbit-tau-p-thr-217-pab-immunocytochemistry-immunofluorescence-2611202518274612.jpg)