| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50051SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-22071SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46806-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89951-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75924SS | |||
| Azide and BSA Free | NBP2-80828 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59339-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52518-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08431 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61122-0.025ml | |||
![Western Blot: Tau Antibody (5B10) [NBP2-50051] - Analysis of tissue lysates using mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot from 48 kDa to 67 kDa with some higher molecular weight big tau Isoforms.](http://images.novusbio.com/fullsize/Tau-Antibody-5B10-Western-Blot-NBP2-50051-img0005.jpg "Western Blot: Tau Antibody (5B10) [NBP2-50051] - Analysis of tissue lysates using mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot from 48 kDa to 67 kDa with some higher molecular weight big tau Isoforms.")
![Immunocytochemistry/Immunofluorescence: Tau Antibody (5B10) [NBP2-50051] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green, and costained with chicken pAb to GFAP, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-50051 stains perikarya, dendrites and axons of neurons, while the GFAP antibody labels astrocytes.](http://images.novusbio.com/fullsize/Tau-Antibody-5B10-Immunocytochemistry-Immunofluorescence-NBP2-50051-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Tau Antibody (5B10) [NBP2-50051] - E18 rat brain cultures were grown for 10 days, fixed and immunostained with NBP2-50051 in red and chicken antibody to MAP2 (NB300-213, green) and for DNA (blue). NBP2-50051 stains the neuronal perikarya, dendrites and axonal process strongly and does not stain non neuronal cells in these cultures. The MAP2 antibody also stains neurons only, but only the perikarya and dendrites. As a result perikarya and dendrites appears yellow, since they contain both tau and MAP2, while axons appear red as they contain only tau.](http://images.novusbio.com/fullsize/Tau-Antibody-5B10-Immunocytochemistry-Immunofluorescence-NBP2-50051-img0003.jpg)
![Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Western-Blot-NB120-22071-img0007.jpg "Western Blot: IkB-alpha Antibody [NB120-22071] - Analysis of IkB-alpha in 20 ug of human Jurkat lysate using 2 ug/ml.")
![Immunohistochemistry-Paraffin: IkB-alpha Antibody [NB120-22071] - Analysis of human breast using IkB-alpha antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-22071-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-46806-img0007.jpg "Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunohistochemistry-Paraffin-NBP2-46806-img0009.jpg)
![Western Blot: HSP70/HSPA1A Antibody [NBP2-46806] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Western-Blot-NBP2-46806-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Hsp70 / Hsc70 Antibody [NBP2-46806] - Staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunohistochemistry-Paraffin-NBP2-46806-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human prostate shows moderate nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunohistochemistry-Paraffin-NBP2-46806-img0008.jpg)
![Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Western-Blot-NBP2-89951-img0005.jpg "Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of Human HSPA1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSPA1A monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunocytochemistry-Immunofluorescence-NBP2-89951-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in cynomolgus macaque testis with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0003.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Analysis of Human HSPA1A expression on HeLa cells. The cells were treated, stained with purified anti-Human HSPA1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Flow-Cytometry-NBP2-89951-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in human hepatoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0002.jpg)
![Immunoprecipitation: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HSPA1A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunoprecipitation-NBP2-89951-img0006.jpg)
![Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Western-Blot-NBP3-16528-img0001.jpg "Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunoprecipitation: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug HSP90 alpha antibody (NBP3-16528). Western blot was performed from the immunoprecipitate using HSP90 alpha antibody (NBP3-16528) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Immunoprecipitation-NBP3-16528-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Rat brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202511411441.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Mouse brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202511402778.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Confocal imaging of paraffin-embedded Human colon cancer tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-5220251255590.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [HSP90 alpha] - Confocal imaging of paraffin-embedded Rat brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202517192942.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [HSP90 alpha] - Confocal imaging of paraffin-embedded Human colon cancer tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-52202517213523.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 alpha Antibody (0R3S6) [HSP90 alpha] - Confocal imaging of paraffin-embedded Mouse brain tissue using HSP90 alpha Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16528_rabbit-hsp90-alpha-mab-0r3s6-5220251718997.jpg)
![Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Western-Blot-NBP2-75924-img0003.jpg "Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: LC3A Antibody (2312D) [NBP2-75924] - This LC3A antibody was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody (Catalog # NBP2-75924) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Immunohistochemistry-Paraffin-NBP2-75924-img0004.jpg)
![Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Western-Blot-NBP2-59339-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Immunocytochemistry-Immunofluorescence-NBP2-59339-img0006.jpg)
![Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cell-Surface-NBP2-59339-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cytometry-NBP2-59339-img0002.jpg)
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0004.jpg "Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)")
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0003.jpg)
![Flow Cytometry: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Flow-Cytometry-NBP2-52518-img0001.jpg)
![Western Blot: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Western-Blot-NBP2-52518-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Analysis of A549 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-Immunofluorescence-NBP2-52518-img0002.jpg)
![ELISA: IkB-alpha Antibody (4D4F2) [NBP2-52518] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-4D4F2-ELISA-NBP2-52518-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody (ASTRO/1974R) [NBP3-07877] - Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S GFAP antibody (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).](http://images.novusbio.com/fullsize/GFAP-Antibody-ASTRO-1974R-Immunocytochemistry-Immunofluorescence-NBP3-07877-img0003.jpg "Immunocytochemistry/Immunofluorescence: GFAP Antibody (ASTRO/1974R) [NBP3-07877] - Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S GFAP antibody (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).")
![Immunohistochemistry-Paraffin: GFAP Antibody (ASTRO/1974R) [NBP3-07877] - Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).](http://images.novusbio.com/fullsize/GFAP-Antibody-ASTRO-1974R-Immunohistochemistry-Paraffin-NBP3-07877-img0002.jpg)
![Flow Cytometry: GFAP Antibody (ASTRO/1974R) [NBP3-07877] - Flow Cytometric Analysis of T98G cells using GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R) followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/GFAP-Antibody-ASTRO-1974R-Flow-Cytometry-NBP3-07877-img0001.jpg)
![Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Western-Blot-NBP2-61122-img0004.jpg "Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61122-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Tissue: Colon Cancer. Species: Human. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody (NBP2-61122) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunohistochemistry-Paraffin-NBP2-61122-img0003.jpg)
![Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Western-Blot-NBP3-16482-img0003.jpg "Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of NIH-3T3 cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of HeLa cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of HeLa cells using HSP70/HSPA1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16482_rabbit-hsp70-hspa1a-mab-5u9a0-52202511411488.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [HSP70/HSPA1A] - Immunofluorescence analysis of HeLa cells using HSP70/HSPA1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16482_rabbit-hsp70-hspa1a-mab-5u9a0-52202517315586.jpg)
![Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239811.jpg "Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Mouse brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239936.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464548.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464554.jpg)