| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70342 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71667 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22194-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB600-1159SS | |||
| Non-Recombinant Monoclonal | NB600-1159 | |||
| BSA Free | NBP3-07012SS | |||
| BSA Free | NBP3-07012 | |||
![Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Western-Blot-NBP2-00492-img0015.jpg "Western Blot: Catalase Antibody (1B8) [NBP2-00492] Analysis of extracts (35ug) from 9 different cell lines by using anti-Catalase monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (OTI1B8) [NBP2-00492] - Mouse hepatocytes stained with catalase antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Catalase-Antibody-OTI1B8-Immunocytochemistry-Immunofluorescence-NBP2-00492-img0019.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human lung tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0010.jpg)
![Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Jurkat cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Flow-Cytometry-NBP2-00492-img0017.jpg)
![Western Blot: Catalase Antibody (1B8) [NBP2-00492] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Catalase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Catalase.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Western-Blot-NBP2-00492-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Catalase Antibody (1B8) [NBP2-00492] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Catalase.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunocytochemistry-Immunofluorescence-NBP2-00492-img0018.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0004.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0006.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human endometrium tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0007.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody (1B8) [NBP2-00492] - Staining of paraffin-embedded Human liver tissue using anti-Catalase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-00492-img0009.jpg)
![Flow Cytometry: Catalase Antibody (1B8) [NBP2-00492] - Analysis of Hela cells, using anti-Catalase antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Catalase-Antibody-1B8-Flow-Cytometry-NBP2-00492-img0016.jpg)
![Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KIF2C monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Western-Blot-NBP2-01064-img0010.jpg "Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KIF2C monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KIF2C .](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunocytochemistry-Immunofluorescence-NBP2-01064-img0011.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Human tonsil using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0009.jpg)
![Flow Cytometry: KIF2C Antibody (OTI1C5) [NBP2-01064] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KIF2C antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Flow-Cytometry-NBP2-01064-img0001.jpg)
![Western Blot: KIF2C Antibody (OTI1C5) [NBP2-01064] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF2C.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Western-Blot-NBP2-01064-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0003.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0004.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0005.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0006.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0007.jpg)
![Immunohistochemistry-Paraffin: KIF2C Antibody (OTI1C5) [NBP2-01064] - Staining of paraffin-embedded Human Kidney tissue using anti-KIF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/KIF2C-Antibody-OTI1C5-Immunohistochemistry-Paraffin-NBP2-01064-img0008.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0001.jpg "Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.")
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of His-tagged protein in E. coli lysates. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0012.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 680 conjugate of NBP2-31055. Detection of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0021.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0013.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 ug/mL).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0014.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of recombinant Treponema denticola major outer sheath His-tagged protein fragments (rMSP, rMSP-C, rMSP-V, rMSP-N) purified from E. coli was performed by loading 5 ug of per well. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 488 conjugate of NBP2-31055. Staining of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a DyLight 488-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scietific ArrayScan or ToxInsight Instrument at 20X magnification.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0024.jpg)
![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Western-Blot-NBP2-22194-img0008.jpg "Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunohistochemistry-Paraffin-NBP2-22194-img0005.jpg)
![Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - An intracellular stain was performed on HeLa cells with Cytokeratin 5 Antibody (2C2) NBP2-22194APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Flow-Cytometry-NBP2-22194-img0009.jpg)
![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mAb against human Cytokeratin 5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Western-Blot-NBP2-22194-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells using Cytokeratin 5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of breast cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Immunohistochemistry-Paraffin-NBP2-22194-img0004.jpg)
![Flow Cytometry: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of HeLa cells using Cytokeratin 5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-Flow-Cytometry-NBP2-22194-img0001.jpg)
![ELISA: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-2C2-ELISA-NBP2-22194-img0006.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0008.jpg "Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0010.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0009.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0009.jpg "Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody at 1/2,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 95 kDa Observed band size: 140/180 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0007.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCAM-1/CD56 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0011.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of SH-SY-5Y cells with NCAM antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Flow-Cytometry-NBP2-66968-img0001.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM on different lysates using anti-NCAM antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Human brain](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0006.jpg)
![Western Blot: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of NCAM-1/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM-1/CD56 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 95 kDa Observed band size: 120/140 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Western-Blot-NBP2-66968-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Staining NCAM in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunocytochemistry-Immunofluorescence-NBP2-66968-img0003.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded zebrafish kidney tissue using anti-NCAM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0005.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (JF1021) [NBP2-66968] - Analysis of paraffin-embedded human tonsil tissue using anti-NCAM-1/CD56 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-JF1021-Immunohistochemistry-Paraffin-NBP2-66968-img0010.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0012.jpg "Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi")
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0007.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0011.jpg)
![Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Flow-Cytometry-NBP2-67327-img0001.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0002.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0003.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0004.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0005.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0008.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0009.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0010.jpg)
![Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Western blot analysis of Cytokeratin 18 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Western-Blot-NBP2-67370-img0011.jpg "Western Blot: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Western blot analysis of Cytokeratin 18 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0016.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Flow-Cytometry-NBP2-67370-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunocytochemistry-Immunofluorescence-NBP2-67370-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0013.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (SZ80-07) [NBP2-67370] - Analysis of paraffin-embedded human colon carcinoma with Rabbit anti-Cytokeratin 18 antibody washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-SZ80-07-Immunohistochemistry-Paraffin-NBP2-67370-img0015.jpg)
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0007.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33134-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Western-Blot-NBP2-33134-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33134-img0004.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Breast cancer tissue stained for Cytokeratin 19 (red) counter stained with DAPI (blue). The Janelia-549 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Immunohistochemistry-Frozen-NBP2-33134-img0001.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0002.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) - Azide and BSA Free [NBP2-33134] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Azide-and-BSA-Free-Simple-Western-NBP2-33134-img0003.jpg)
![Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Western-Blot-NBP2-33250-img0004.jpg "Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).")
![Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - ICC/IF analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-33250-img0002.jpg)
![Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Immunohistochemical analysis of bouin-fixed, paraffin-embedded chronic lymphocytic leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Immunohistochemistry-Paraffin-NBP2-33250-img0003.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0007.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1-Azide-Free-Flow-Cytometry-NBP2-33250-img0006.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0018.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0004.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - IP Sample: Raji whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 ug of Histone H3 antibody15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16842-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody (NBP2-16842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16842_rabbit-polyclonal-hist1h3a-antibody-9102024202460.jpg)
