| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45030 | |||
| Azide and BSA Free | NBP2-34403-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2618SS | |||
| Azide and BSA Free | NBP2-80817 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47816-0.1mg | |||
| IHC-Prediluted | NBP2-48156 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36491SS | |||
| Azide and BSA Free | NBP2-80662 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54790SS | |||
| Azide and BSA Free | NBP2-80644 | |||
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." alt="Collagen IV was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha 1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79899 | |||
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0010.jpg "Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0011.jpg)
![Flow Cytometry: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Flow-Cytometry-NBP2-67182-img0001.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0004.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human lung cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0007.jpg)
![Western blot analysis on different lysates with NBP2-67182 at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: HL-60 cell lysate Lane 5: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67182 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67182_-western-blot-639204586673699802.jpg)
![Western blot analysis on different lysates with NBP2-67182 at 1/5,000 dilution. Lane 1: Rat spleen tissue lysate Lane 2: Mouse spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67182 at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67182_-western-blot-639204597491319190.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Staining of a 9 days old zebrafish embryo](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunocytochemistry-Immunofluorescence-NBP1-97712-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Staining of a 9 days old zebrafish embryo")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section with NBP1-97712 (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Paraffin-NBP1-97712-img0020.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Flow Cytometry: Cytokeratin 19 Antibody (RCK108) [DyLight 650] [NBP1-97712C] - An intracellular stain was performed on HT-29 cells with Cytokeratin 19 (RCK108) NBP1-97712C (blue) and a matched isotype control NBP1-97005C (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight650. Image using the DyLight 650 form of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Flow-Cytometry-NBP1-97712-img0014.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Analysis using the Unpurified version of NBP1-97712. Immunohistochemistry on frozen section of human liver bile duct epithelium](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Frozen-NBP1-97712-img0010.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on a frozen tissue sections of human kidney with NBP1-97712 (RCK108; dilution 1:500).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Frozen-NBP1-97712-img0015.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Immunohistochemistry on frozen tissue sections of human colon with NBP1-97712 (RCK108; dilution 1:500).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Frozen-NBP1-97712-img0017.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human small intestine paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Paraffin-NBP1-97712-img0018.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (RCK108) [NBP1-97712] - Indirect immunoperoxidase staining of human placenta paraffin tissue section with NBP1-97712 (RCK108; Mouse anti Cytokeratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-RCK108-Immunohistochemistry-Paraffin-NBP1-97712-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 Antibody (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunocytochemistry-Immunofluorescence-NBP2-34355-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 Antibody (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (20X) stained with Cytokeratin 14 MAb (SPM263).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunohistochemistry-Paraffin-NBP2-34355-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (10X) stained with Cytokeratin 14 MAb (SPM263).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunohistochemistry-Paraffin-NBP2-34355-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) - Human Tonsil tissue with epithelial CK14 expression as monochromatic image. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-34355_mouse-monoclonal-cytokeratin-14-antibody-spm263-17720241003233.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Western-Blot-NBP2-43560-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody [812] diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Immunohistochemistry-Frozen-NBP2-43560-img0006.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [812] diluted at 1:250. Blue: DAPI.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Immunohistochemistry-Frozen-NBP2-43560-img0001.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Western-Blot-NBP2-43560-img0002.jpg)
![Sandwich ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using Vimentin Antibody (812) as capture antibody at concentration of 5 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Sandwich-ELISA-NBP2-43560-img0011.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-910202420131230.jpg)
![ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using NBP2-43560 as capture antibody at concentration of 5 ug/mL and detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-91020242041256.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP2-43560) diluted at 1:500000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-910202420131251.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:3000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-2611202519369.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-2611202518434512.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-261120251820010.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:75000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-261120251837248.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (P2W7) [NB100-2618] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Integrin alpha V/CD51 Antibody [P2W7] (NB100-2618) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Integrin-alpha-V-CD51-Antibody-P2W7-Immunocytochemistry-Immunofluorescence-NB100-2618-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin alpha V/CD51 Antibody (P2W7) [NB100-2618] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Integrin alpha V/CD51 Antibody [P2W7] (NB100-2618) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Western Blot Analysis of human Ovary tissue using Caldesmon/CALD1 antibody (h-CALD).](http://images.novusbio.com/fullsize/Caldesmon-CALD1-Antibody-h-CALD-Western-Blot-NBP2-44421-img0008.jpg "Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Western Blot Analysis of human Ovary tissue using Caldesmon/CALD1 antibody (h-CALD).")
![Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Analysis of Human uterus tissue using Caldesmon/CALD1 antibody (h-CALD). Image from verified customer review.](http://images.novusbio.com/fullsize/Caldesmon-CALD1-Antibody-h-CALD-Immunohistochemistry-Paraffin-NBP2-44421-img0007.jpg)
![Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Human Uterus stained with Caldesmon Monoclonal Antibody (h-CALD).](http://images.novusbio.com/fullsize/Caldesmon-CALD1-Antibody-h-CALD-Immunohistochemistry-Paraffin-NBP2-44421-img0001.jpg)
![Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Simple Western lane view shows a specific band for Caldesmon/CALD1 in 0.2 mg/ml of h. Aorta lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caldesmon-CALD1-Antibody-h-CALD-Simple-Western-NBP2-44421-img0002.jpg)
![Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Simple Western lane view shows a specific band for Caldesmon/CALD1 in 0.2 mg/ml of h. Uterus lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caldesmon-CALD1-Antibody-h-CALD-Simple-Western-NBP2-44421-img0003.jpg)
![Simple Western: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Electropherogram image of the corresponding Simple Western lane. Caldesmon/CALD1 antibody was used at 10 ug/ml dilution of h. Aorta and h. Uterus lysates(s) respectively.](http://images.novusbio.com/fullsize/Caldesmon-CALD1-Antibody-h-CALD-Simple-Western-NBP2-44421-img0006.jpg)
![Western Blot: CD98 Antibody (1C11.7E3) [NBP2-36491] - Western blot analysis of human CD98 in recombinant protein, A431, Jurkat, HeLa and HepG2 lysates at 2.0 ug/ml.](http://images.novusbio.com/fullsize/CD98-Antibody-1C11-7E3-Western-Blot-NBP2-36491-img0001.jpg "Western Blot: CD98 Antibody (1C11.7E3) [NBP2-36491] - Western blot analysis of human CD98 in recombinant protein, A431, Jurkat, HeLa and HepG2 lysates at 2.0 ug/ml.")
![Immunohistochemistry-Paraffin: CD98 Antibody (1C11.7E3) [NBP2-36491] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney using CD98 antibody (clone 1C11.7E3) at 1:300 dilution. The antibody generated a distinct/intense membranous staining with relatively weaker cytoplasmic signal, and the immunostaining was exclusively observed in tubular epithelial cells with no signal in the Bowman's capsules.](http://images.novusbio.com/fullsize/CD98-Antibody-1C11-7E3-Immunohistochemistry-Paraffin-NBP2-36491-img0003.jpg)
![Flow (Cell Surface): CD98 Antibody (1C11.7E3) [NBP2-36491] - Flow cytometry analysis of human CD98 in hPBMC. Primary antibody was used at 2.5 ug / million cells. Goat anti-mouse IgG (PE) was used at 0.5 ug / million cells. The red shift line represents the primary antibody, the blue line represents the cells alone and the green line represents the isotype control.](http://images.novusbio.com/fullsize/CD98-Antibody-1C11-7E3-Flow-Cell-Surface-NBP2-36491-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0023.jpg "Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CD36 Monoclonal antibody (catalog number NBP2-54790) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). A specific band was detected for CD36/SR-B3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD36 Antibody (1283D) [NBP2-54790] - CD36 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human CD36/SR-B3 Monoclonal Antibody for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocyte membranes.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunohistochemistry-Paraffin-NBP2-54790-img0002.jpg)
![Western Blot: CD36 Antibody (1283D) [NBP2-54790] - Total protein from human skin and adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-CD36 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Western-Blot-NBP2-54790-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD36 Antibody (1283D) [NBP2-54790] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CD36 (1283D) at 5.0 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective..](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-1283D-Immunocytochemistry-Immunofluorescence-NBP2-54790-img0003.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-3102024161365.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - LYN phosphorylates DHHC5 & is required for the endocytosis of FAs.a 3T3-L1 adipocytes pretreated with serum-free medium for 4 h & PP2 (15 μM) for 1 h, followed by treatment with BSA or oleate (100 μM) for 5 min. Cells harvested & immunoprecipitated with anti-DHHC5 antibody to detect phosphorylation of Tyr91. b 3T3-L1 adipocytes transduced with indicated shRNA set up & treated with oleate for 5 min. Cells harvested to detect Tyr91 phosphorylation as in (a). c On day 0, HEK293T cells set up at 2.5 × 105 cells per 6-cm dish. On day 2, cells transfected with 0.5 μg of WT, Y91E, or Y91F of DHHC5-Flag/pCDH-puro and/or 0.5 μg LYN/pCDNA3.3. On day 3, cells harvested & immunoprecipitated with anti-Flag M2 beads to detect phosphorylation of DHHC5 (pY). Control & LYN knockdown adipocytes pretreated & treated with BSA or oleate (100 μM) for 1 h, followed by immunostaining (d), surface biotinylation (e), & Acyl-RAC (f) assays. Control & CD36 knockdown 3T3-L1 adipocytes pretreated & treated with oleate (100 μM) for indicated time (g) or 5 min (h). Cells harvested & subjected to immunoprecipitation of LYN to detect phosphorylation of LYN (Y396). i, j WT & Cd36−/− SVFs set up & subjected to FA uptake as in Fig. 3h, i, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 20 cells. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. k WT & Cd36−/− adipocytes set up & subjected to 3H-oleate uptake as in Fig. 3j, except that cells pretreated with PP2 (15 μM). Each value represents mean ± SEM obtained from 3samples. Two-sided Student’s t test was performed between DMSO & PP2 treated cells. These experiments repeated at least twice. Source data provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114783.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416121990.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-31020241612242.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114740.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - FAs trigger internalization of CD36.a, b On day 8 of differentiation, 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated oleate (100 μM) for indicated time. a One set of cells was subjected to immunostaining with anti-CD36 & anti-CAV1 antibodies. LipidTOX was used to label lipid droplets. Images were taken under a Zeiss LSM-780 microscope in a 3D Z-stack mode & reconstructed using Imaris 9.2.0. b The other set of cells was subjected to surface biotinylation assay & blotted with indicated antibodies. c, d On day 4 of differentiation, 3T3-L1 cells were infected with lentivirus encoding scrambled shRNA or shRNAs against CD36 or CAV1. On day 5, cells were selected with 5 μg/ml puromycin. On day 8, cells were pretreated as in (a) & treated with oleate (100 μM) for 4 h, followed by immunostaining with anti-CD36 & anti-CAV1 antibodies (c), or surface biotinylation assay (d). e, f 3T3-L1 adipocytes were pretreated as in (a) & treated with BSA-conjugated FAs with different chain lengths or saturation (100 μM) for 4 h. Cells were subjected to immunostaining with anti-CD36 antibody (e), or surface biotinylation assay (f). After oleate treatment for 4 h, 3T3-L1 adipocytes were switched to serum-free medium for indicated time & harvested for immunostaining (g) & surface biotinylation (h). The scale bars were as indicated in each figure. These experiments were repeated at least three times. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416114741.jpg)
![Western Blot: CD36 Antibody (1283D) - BSA Free [NBP2-54790] - CD36-mediated caveolar endocytosis transports FAs into cells.a–c 3T3-L1 adipocytes were pretreated with serum-free medium for 4 h, & then treated with BSA-conjugated PacFA (50 μM) for 20 min, followed by UV crosslinking on ice for 30 min. a, b Cells were subjected to click chemistry using an N3-Alexa Fluro 488, & immunostained with anti-CD36 antibody. Colocalization of PacFA & CD36 was quantified from 24 cells over three independent experiments & plotted in (b). The value represents mean ± SEM. c Cells were lysed & subjected to click chemistry assay using N3-biotin. PacFA-labeled proteins were captured with streptavidin beads & subjected to western blot using anti-CD36 & anti-FABP4 antibodies. d WT, Cav1−/−, Cd36−/−, & Cav1−/−;Cd36−/− SVFs were isolated & differentiated into adipocytes & treated with 100 μM 3H-oleate (specific activity, 2268 dpm/nmol) for 1 h. Lipid fractions were extracted from the cells & subjected to scintillation counting. The radioactive counting was normalized to protein content. Each value represents mean ± SEM obtained from three samples. Two-sided Student’s t test was performed between WT & each of the knockout cells. These experiments were repeated twice. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32958780), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-54790_rabbit-monoclonal-cd36-antibody-1283d-310202416113520.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0006.jpg "Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [NBP1-60140] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-Frozen-NBP1-60140-img0007.jpg)
![Immunohistochemistry: F4/80 Antibody (BM8) [NBP1-60140] - Expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-NBP1-60140-img0003.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Analysis using the FITC conjugate of NBP1-60140. Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 FITC.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0005.jpg)
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # ![COL4A1 antibody [HL1351] detects COL4A1 protein at vesicle by immunofluorescent analysis.</br>Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:500.</br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25416_rabbit-collagen-iv-alpha-1-mab-hl1351-azide-and-bsa-free-immunocytochemistry-immunofluorescence-2611202518301211.jpg)
![COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.<br>Sample: Paraffin-embedded mouse placenta.<br>COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:100.<br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25416_rabbit-collagen-iv-alpha-1-mab-hl1351-azide-and-bsa-free-immunohistochemistry-paraffin-2611202518413915.jpg)
![COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.<br>Sample: Paraffin-embedded mouse placenta.<br>COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:100.<br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25416_rabbit-collagen-iv-alpha-1-mab-hl1351-azide-and-bsa-free-immunohistochemistry-paraffin-261120251823623.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25416_rabbit-collagen-iv-alpha-1-mab-hl1351-azide-and-bsa-free-western-blot-261120251834119.jpg)
![COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane by immunohistochemical analysis.<br>Sample: Paraffin-embedded human kidney.<br>COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:50.<br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25416_rabbit-collagen-iv-alpha-1-mab-hl1351-azide-and-bsa-free-immunohistochemistry-paraffin-261120251850422.jpg)
![Western Blot: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Western Blot Analysis of A431 cell lysate using S100A2 Antibody (CPTC-S100A2-2).](http://images.novusbio.com/fullsize/S100A2-Antibody-CPTC-S100A2-2-Western-Blot-NBP2-79764-img0006.jpg "Western Blot: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Western Blot Analysis of A431 cell lysate using S100A2 Antibody (CPTC-S100A2-2).")
![Immunocytochemistry/Immunofluorescence: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Immunofluorescence Analysis of A549 cells labeling S100A2 with S100A2 Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)](http://images.novusbio.com/fullsize/S100A2-Antibody-CPTC-S100A2-2-Immunocytochemistry-Immunofluorescence-NBP2-79764-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Formalin-fixed, paraffin-embedded human Placenta stained with S100A2 Antibody (CPTC-S100A2-2).](http://images.novusbio.com/fullsize/S100A2-Antibody-CPTC-S100A2-2-Immunohistochemistry-Paraffin-NBP2-79764-img0004.jpg)
![Flow Cytometry: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Flow Cytometric Analysis of trypsinised HeLa cells. S100A2 Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/S100A2-Antibody-CPTC-S100A2-2-Flow-Cytometry-NBP2-79764-img0001.jpg)
![Immunohistochemistry-Paraffin: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Formalin-fixed, paraffin-embedded human Skin stained with S100A2 Antibody (CPTC-S100A2-2).](http://images.novusbio.com/fullsize/S100A2-Antibody-CPTC-S100A2-2-Immunohistochemistry-Paraffin-NBP2-79764-img0003.jpg)
![Protein Array: S100A2 Antibody (CPTC-S100A2-2) [NBP2-79764] - Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A2 Antibody (CPTC-S100A2-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/S100A2-Antibody-CPTC-S100A2-2-Protein-Array-NBP2-79764-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0005.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Biopsies of deparaffinized Human melanoma tissue. Cells were probed without (control) or with or an antibody recognizing Vimentin](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0003.jpg)
![Western Blot: Vimentin Antibody (J144) [NB100-74564] - Analysis of rat brain cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Western-Blot-NB100-74564-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0006.jpg)
