| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27406SS | |||
| Azide and BSA Free | NBP2-80834 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96887-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71239 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24762SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38231-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76554-0.025mg | |||
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Western-Blot-NBP2-27406-img0009.jpg "Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0008.jpg)
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Western-Blot-NBP2-27406-img0001.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands, whereas the surrounding fibromuscular stroma cells did not develop any staining.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0002.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0003.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0004.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0006.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0007.jpg)
![Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Western-Blot-NB200-335-img0006.jpg "Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NB200-335-img0008.jpg)
![Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunoprecipitation-NB200-335-img0013.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-2472024125914..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Analysis in human adrenal gland and skin tissues using NBP1-87498 antibody. Corresponding FLOT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-FLOT1 antibody. Corresponding FLOT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0012.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0009.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-NBP1-87498-img0013.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413032..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413122..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Staining of human Stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-247202413216..jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] -Staining of human Skin shows very weak membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87498_rabbit-polyclonal-flotillin-1-antibody-immunohistochemistry-paraffin-24720241338..jpg)
![Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Western-Blot-NB110-96887-img0013.jpg "Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Analysis using Rabbit Anti-ERK1 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0011.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NB110-96887] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-96887-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody [NB110-96887] - Bouin's fixed paraffin embedded backskin sections of transgenic mice. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-Paraffin-NB110-96887-img0003.jpg)
![Immunohistochemistry: ERK1/2 Antibody [NB110-96887] - Immunohistochemistry analysis using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/ERK1-2-Antibody-Immunohistochemistry-NB110-96887-img0014.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0008.jpg "Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Analysis using MAPK3 mAb against human ERK1 (AA: 9-143) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0006.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis using ERK1 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Western-Blot-NBP2-22203-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![ELISA: ERK1 Antibody (1E5) [NBP2-22203] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/ERK1-Antibody-1E5-ELISA-NBP2-22203-img0005.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0007.jpg "Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0006.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Western-Blot-NBP2-45814-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0002.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunohistochemistry-NBP2-45814-img0005.jpg)
![Immunofluorescence: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Immunofluorescence-NBP2-45814-img0001.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0002.jpg "Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.")
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERK1.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71239-img0008.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0007.jpg)
![Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Western-Blot-NBP2-71239-img0001.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0003.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0004.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0005.jpg)
![Immunohistochemistry: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/ERK1-Antibody-OTI4D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71239-img0006.jpg)
![Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Western-Blot-NBP2-67378-img0008.jpg "Western Blot: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562")
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0007.jpg)
![Flow Cytometry: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Flow-Cytometry-NBP2-67378-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunocytochemistry-Immunofluorescence-NBP2-67378-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK1/2 Antibody (SP05-09) [NBP2-67378] - Analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK1-2-Antibody-SP05-09-Immunohistochemistry-Paraffin-NBP2-67378-img0006.jpg)
![Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Western-Blot-NBP2-24762-img0007.jpg "Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.")
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP2-24762] - Human liver tissue using an isotype control (top left) and NBP2-24762 (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Immunohistochemistry-Paraffin-NBP2-24762-img0006.jpg)
![Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Sphingolipid Receptor Edg3/S1P3 in kidney lysate. Lane 1 shows pre-immune sera. Lanes 2 and 3 show NBP2-24762 tested on human kidney (2 ug/ml) and mouse kidney (1 ug/ml) lysate.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Western-Blot-NBP2-24762-img0005.jpg)
![Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252844.jpg "Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western blot analysis of various lysates, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunoprecipitation: Flotillin-1 Antibody [NBP3-38231] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Flotillin-1 antibody. Western blot was performed from the immunoprecipitate using Flotillin-1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-1012202411545540.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121097.jpg)
![Immunohistochemistry: Flotillin-1 Antibody [NBP3-38231] - Immunohistochemistry analysis of paraffin-embedded Rat heart using [KO Validated] Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-101220241252843.jpg)
![Immunocytochemistry/ Immunofluorescence: Flotillin-1 Antibody [NBP3-38231] - Immunofluorescence analysis of NIH/3T3 cells using Flotillin-1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-10122024121062.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Flotillin-1 Antibody [NBP3-38231] - Western Blot analysis of lysates from wild type (WT) and Flotillin-1 knockout (KO) 293T cells, using [KO Validated] Flotillin-1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38231_rabbit-flotillin-1-pab-311202516593618.jpg)
![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Rat Lung Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0006.jpg "Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Rat Lung Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: LIF Antibody [NBP1-76554] - 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0001.jpg)
![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0003.jpg)
![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation with Recombinant Protein. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2ug/mL, 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0004.jpg)
![Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/LIF-Antibody-Western-Blot-NBP1-76554-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: LIF Antibody - BSA Free [NBP1-76554] - Validation of LIF in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76554_rabbit-polyclonal-lif-antibody-2711202420272450.jpg)
![Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NB100-1043-img0001.jpg "Western Blot: Flotillin-1 Antibody [NB100-1043] - Staining of K562 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by Chemiluminescence.")
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0003.jpg)
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NB100-1043] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Immunohistochemistry-Paraffin-NB100-1043-img0002.jpg)