| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-724-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41263-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04585-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-21805-0.1ml | |||
![Western Blot: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS antibody [HL1730] (NBP3-25503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25503_rabbit-hgs-mab-hl1730-azide-and-bsa-free-2120249415972.jpg "Western Blot: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS antibody [HL1730] (NBP3-25503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - HGS antibody [HL1730] detects HGS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HGS stained by HGS antibody [HL1730] (NBP3-25503) diluted at 1:250. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25503_rabbit-hgs-mab-hl1730-azide-and-bsa-free-2120249371551.jpg)
![Immunohistochemistry-Paraffin: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - HGS antibody [HL1730] detects HGS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog pancreas. HGS stained by HGS antibody [HL1730] (NBP3-25503) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25503_rabbit-hgs-mab-hl1730-azide-and-bsa-free-2120249432828.jpg)
![Immunohistochemistry-Paraffin: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - HGS antibody [HL1730] detects HGS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat pancreas. HGS stained by HGS antibody [HL1730] (NBP3-25503) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25503_rabbit-hgs-mab-hl1730-azide-and-bsa-free-212024941474.jpg)
![Western Blot: HGS Antibody (HL1730) - Azide and BSA Free [NBP3-25503] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS antibody [HL1730] (NBP3-25503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25503_rabbit-hgs-mab-hl1730-azide-and-bsa-free-2120249393580.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-212024941427.jpg "Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-261120251839316.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunohistochemical analysis.Sample: EGFRvIII transfected 293T cell FFPE Cell Pellet Block.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunohistochemistry-paraffin-26112025195717.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.Blue: Fluoroshield with DAPI .*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-26112025199124.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518224316.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518463713.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.Blue: Fluoroshield with DAPI .*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-261120251818503.jpg)
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0003.jpg "Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.")
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Staining of A431 cells with RAT ANTI HUMAN EGF RECEPTOR (NB 600-724) visualised with F(ab')2 RABBIT ANTI RAT IgG: FITC secondary.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0001.jpg)
![Western Blot: ARF6 Antibody [NBP2-41263] - Analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/ARF6-Antibody-Western-Blot-NBP2-41263-img0001.jpg "Western Blot: ARF6 Antibody [NBP2-41263] - Analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: ARF6 Antibody - BSA Free [NBP2-41263] - Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41263_rabbit-polyclonal-arf6-antibody-2711202420324836.jpg)
![Immunohistochemistry: ARF6 Antibody - BSA Free [NBP2-41263] - Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41263_rabbit-polyclonal-arf6-antibody-2711202420212343.jpg)
![Western Blot: Septin-3 Antibody [NB100-1023] - NB100-1023 (0.5ug/ml) staining of Human Cerebellum (A), (0.1ug/ml) Mouse (B) and (0.03ug/ml) Rat (C) Brain lysate (35ug protein in RIPA buffer).. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-3-Antibody-Western-Blot-NB100-1023-img0006.jpg "Western Blot: Septin-3 Antibody [NB100-1023] - NB100-1023 (0.5ug/ml) staining of Human Cerebellum (A), (0.1ug/ml) Mouse (B) and (0.03ug/ml) Rat (C) Brain lysate (35ug protein in RIPA buffer).. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-3-Antibody-Immunohistochemistry-Paraffin-NB100-1023-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-3-Antibody-Immunohistochemistry-Paraffin-NB100-1023-img0003.jpg)
![Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-3-Antibody-Immunohistochemistry-Paraffin-NB100-1023-img0004.jpg)
![Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Western-Blot-NBP3-04585-img0005.jpg "Western Blot: KIFC1 Antibody [NBP3-04585] - Analysis of extracts of various cell lines, using KIFC1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: KIFC1 Antibody [NBP3-04585] - Analysis of U-2 OS cells using KIFC1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04585-img0001.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded mouse testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0004.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded rat testis using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0002.jpg)
![Immunohistochemistry-Paraffin: KIFC1 Antibody [NBP3-04585] - Paraffin-embedded human colon carcinoma using KIFC1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/KIFC1-Antibody-Immunohistochemistry-Paraffin-NBP3-04585-img0003.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0004.jpg "Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of Rat lung, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad2 Rabbit mAb (NBP3-15671) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunocytochemistry-Immunofluorescence-NBP3-15671-img0002.jpg)
![Western Blot: Smad2 Antibody (2E3D8) [NBP3-15671] - Western blot analysis of extracts of various cell lines, using Smad2 antibody (NBP3-15671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Western-Blot-NBP3-15671-img0003.jpg)
![Immunoprecipitation: Smad2 Antibody (2E3D8) [NBP3-15671] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Smad2 antibody (NBP3-15671). Western blot was performed from the immunoprecipitate using Smad2 antibody (NBP3-15671) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Smad2-Antibody-2E3D8-Immunoprecipitation-NBP3-15671-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody (2E3D8) [Smad2] - Western blot analysis of lysates from wild type (WT) and Smad2 knockdown (KD) HeLa cells using [KD Validated] Smad2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-52202517304995.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (2E3D8) [Smad2] - Confocal imaging of HeLa cells using [KD Validated] Smad2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15671_rabbit-smad2-mab-2e3d8-522025173455.jpg)
![Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Western-Blot-NBP3-15560-img0006.jpg "Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560] - Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunocytochemistry-Immunofluorescence-NBP3-15560-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Knockout-Validated-NBP3-15560-img0001.jpg)
![Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (NBP3-15560). Western blot was performed from the immunoprecipitate using APP antibody (NBP3-15560) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunoprecipitation-NBP3-15560-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [APP] - Confocal imaging of paraffin-embedded 5XFAD Mouse brain and Mouse brain using [KO Validated] APP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15560_rabbit-app-mab-3a8s2-52202517304935.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunofluorescence-NBP2-21805-img0002.jpg "Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (GC-4) [NBP2-21805] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with Monoclonal Anti-N-Cadherin, Clone: GC-4. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Immunohistochemistry: N-Cadherin Antibody (GC-4) [NBP2-21805] - Frozen acetone fixed chicken cardiac sections stained with Monoclonal Anti-N-Cadherin antibody produced in Mouse clone GC-4: followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-GC-4-Immunohistochemistry-NBP2-21805-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-immunocytochemistry-immunofluorescence-2972024113947..jpg "Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594,was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/1,000 dilution.Lane 1: Raji cell lysate (10 ug/Lane)Lane 2: Mouse lung tissue lysate (20 ug/Lane)Lane 3: Rat lung tissue lysate (20 ug/Lane)Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114129..jpg)
![Western Blot: B7-2/CD86 Antibody (SJ20-00) [NBP2-67417] - Analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/2,000 dilution.Lane 1: Daudi cell lysateLane 2: Raji cell lysateLane 3: Ramos cell lysateLane 4: Jurkat cell lysateLane 5: K-562 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 38 kDaObserved band size: 70 kDaExposure time: 2 minutes 37 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67417_rabbit-monoclonal-b7-2-cd86-antibody-sj20-00-western-blot-2972024114258..jpg)
![Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27197-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15587-img0008.jpg "Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human papillary adenocarcinoma. BIN1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0005.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0006.jpg)
![Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BIN1-Antibody-Western-Blot-NBP2-15587-img0007.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BIN1-Antibody-Immunohistochemistry-Paraffin-NBP2-15587-img0003.jpg)